NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	EGT69_RS18300	Genome accession	NZ_CP078018
Coordinates	460322..461182 (-)	Length	286 a.a.
NCBI ID	WP_004916856.1	Uniprot ID	S7WGQ5
Organism	Acinetobacter junii strain AJ_351
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 455322..466182

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGT69_RS02110 (EGT69_002110)	-	457297..457479 (+)	183	WP_125297385.1	hypothetical protein	-
EGT69_RS02115 (EGT69_002115)	-	457515..457841 (+)	327	WP_004958605.1	pyrimidine/purine nucleoside phosphorylase	-
EGT69_RS02120 (EGT69_002120)	rlmB	458033..458782 (+)	750	WP_004916859.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
EGT69_RS02125 (EGT69_002125)	-	458813..459724 (+)	912	WP_125297384.1	DMT family transporter	-
EGT69_RS02130 (EGT69_002130)	coaE	459721..460320 (-)	600	WP_125297383.1	dephospho-CoA kinase	-
EGT69_RS18300 (EGT69_002135)	pilD	460322..461182 (-)	861	WP_004916856.1	prepilin peptidase	Machinery gene
EGT69_RS02140 (EGT69_002140)	pilC	461182..462408 (-)	1227	WP_004916853.1	type II secretion system F family protein	Machinery gene
EGT69_RS02145 (EGT69_002145)	pilB	462433..464157 (-)	1725	WP_125297382.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EGT69_RS02150 (EGT69_002150)	tpiA	464503..465294 (+)	792	WP_005403576.1	triose-phosphate isomerase	-
EGT69_RS02155 (EGT69_002155)	secG	465307..465636 (+)	330	WP_004916847.1	preprotein translocase subunit SecG	-

Sequence

Protein

Download Length: 286 a.a. Molecular weight: 32013.53 Da Isoelectric Point: 6.8236

                                >NTDB_id=586396 EGT69_RS18300 WP_004916856.1 460322..461182(-) (pilD) [Acinetobacter junii strain AJ_351]

MQSFFFYFLENPTALYLAVGLISLCIGSFLNVVIFRTPKMMEQQWQQECQILLHPEQPIIDQSKLTLSQPVSTCPECQTP

IKWFQNIPLISWLALRGKCGTCQNPISIRYPLIELLTMICSLIVVAVFGATIQMLFGLILTWTLIALTFIDFDTQLLPDR

FTLPLAALGLGINTFAIYTTASAAIWGYLIGFLCLWLVYYLFKIITGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSM

VGAIIGIMLMKMRGENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGG

Nucleotide

Download Length: 861 bp

                                >NTDB_id=586396 EGT69_RS18300 WP_004916856.1 460322..461182(-) (pilD) [Acinetobacter junii strain AJ_351]

ATGCAATCTTTTTTCTTTTACTTCCTTGAAAATCCAACTGCACTTTACCTTGCAGTTGGATTAATCAGTCTGTGCATTGG

CAGTTTTTTAAATGTTGTGATCTTTCGCACTCCAAAAATGATGGAGCAGCAATGGCAGCAAGAATGCCAAATACTTTTGC

ACCCTGAACAGCCGATCATAGATCAGAGCAAGTTAACATTAAGTCAGCCAGTTTCCACCTGTCCTGAGTGTCAAACGCCA

ATTAAATGGTTCCAAAATATTCCTCTTATTAGTTGGTTAGCACTCCGTGGTAAATGTGGCACTTGCCAGAATCCAATTAG

CATTCGCTACCCGTTGATTGAATTACTTACTATGATCTGTTCATTGATCGTTGTGGCAGTATTCGGTGCAACAATTCAGA

TGCTTTTCGGCTTAATTCTAACGTGGACACTTATTGCACTCACTTTTATTGATTTTGATACACAATTACTACCTGATCGC

TTTACGCTACCTTTAGCAGCATTAGGTCTAGGTATTAATACTTTTGCAATCTATACAACGGCTAGTGCTGCGATCTGGGG

CTATTTAATCGGTTTTCTATGTCTATGGCTTGTGTATTACCTATTTAAAATTATCACCGGTAAAGAAGGCATGGGCTATG

GTGATTTTAAATTGCTTGCAGCCCTTGGTGCATGGATGGGTCCGATGATGTTACCCCTCATCGTATTACTCTCTTCTATG

GTAGGCGCAATTATTGGTATTATGCTGATGAAAATGCGCGGTGAAAATCAACCTTTTGCATTTGGTCCTTATATTGCAAT

TGCTGGTTGGATTGCCTTTCTATGGGGTGAGCAAATTATGAAAGTATATCTTGGGGGTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	S7WGQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter nosocomialis M2	81.469	100	0.815
pilD	Acinetobacter baumannii D1279779	81.119	100	0.811
pilD	Vibrio cholerae strain A1552	52.083	100	0.524
pilD	Vibrio campbellii strain DS40M4	51.128	93.007	0.476
pilD	Neisseria gonorrhoeae MS11	46.154	95.455	0.441

Detailed information NTG0586396