Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   M0D10_RS00285 Genome accession   NZ_CP096207
Coordinates   48811..49275 (+) Length   154 a.a.
NCBI ID   WP_023129290.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa TBCF10839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43811..54275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D10_RS00260 (M0D10_00255) yacG 43981..44181 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  M0D10_RS00265 (M0D10_00260) coaE 44178..44789 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  M0D10_RS00270 (M0D10_00265) pilD 44786..45658 (-) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M0D10_RS00275 (M0D10_00270) pilC 45659..46876 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  M0D10_RS00280 (M0D10_00275) pilB 46880..48580 (-) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0D10_RS00285 (M0D10_00280) pilA/pilAI 48811..49275 (+) 465 WP_023129290.1 pilin Machinery gene
  M0D10_RS00290 (M0D10_00285) - 49433..50746 (+) 1314 WP_015648064.1 O-antigen ligase -
  M0D10_RS00300 (M0D10_00295) nadC 50927..51775 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16148.47 Da        Isoelectric Point: 8.9986

>NTDB_id=586095 M0D10_RS00285 WP_023129290.1 48811..49275(+) (pilA/pilAI) [Pseudomonas aeruginosa TBCF10839]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=586095 M0D10_RS00285 WP_023129290.1 48811..49275(+) (pilA/pilAI) [Pseudomonas aeruginosa TBCF10839]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364