Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   I872_RS09720 Genome accession   NC_021175
Coordinates   1974268..1974705 (-) Length   145 a.a.
NCBI ID   WP_015605916.1    Uniprot ID   -
Organism   Streptococcus cristatus AS 1.3089     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1969268..1979705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I872_RS09700 (I872_09710) - 1970233..1971120 (+) 888 WP_041826841.1 RluA family pseudouridine synthase -
  I872_RS09705 (I872_09715) - 1971602..1972798 (-) 1197 Protein_1906 acetate kinase -
  I872_RS09710 (I872_09720) comYH 1972834..1973799 (-) 966 WP_015605914.1 class I SAM-dependent methyltransferase Machinery gene
  I872_RS09715 (I872_09725) comGG 1973880..1974287 (-) 408 WP_015605915.1 competence type IV pilus minor pilin ComGG -
  I872_RS09720 (I872_09730) comGF/cglF 1974268..1974705 (-) 438 WP_015605916.1 competence type IV pilus minor pilin ComGF Machinery gene
  I872_RS09725 (I872_09735) comGE/cglE 1974689..1974958 (-) 270 WP_015605917.1 competence type IV pilus minor pilin ComGE Machinery gene
  I872_RS09730 (I872_09740) comYD 1974948..1975382 (-) 435 WP_041826842.1 competence type IV pilus minor pilin ComGD Machinery gene
  I872_RS09735 (I872_09745) comYC 1975342..1975659 (-) 318 WP_015605919.1 competence type IV pilus major pilin ComGC Machinery gene
  I872_RS09740 (I872_09750) comYB 1975656..1976672 (-) 1017 WP_015605920.1 competence type IV pilus assembly protein ComGB Machinery gene
  I872_RS09745 (I872_09755) comYA 1976620..1977561 (-) 942 WP_015605921.1 competence type IV pilus ATPase ComGA Machinery gene
  I872_RS09750 (I872_09760) - 1977646..1978017 (-) 372 WP_015605922.1 DUF1033 family protein -
  I872_RS09755 - 1978152..1979420 (-) 1269 WP_041826843.1 CapA family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16945.48 Da        Isoelectric Point: 9.8258

>NTDB_id=58482 I872_RS09720 WP_015605916.1 1974268..1974705(-) (comGF/cglF) [Streptococcus cristatus AS 1.3089]
MLKNVKIKAFTLLETLVALLVLSGSMLVFQSLTKLLAFELRQQQENKQQEWLLFVDQLETELSRSQFDRVENDKIYVKQD
GKNIALGKSRSDDFRKTDASGRGYQPMVYGLKAAPITQKGNLVRFRFQFENDLEREYIYRVQDKS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=58482 I872_RS09720 WP_015605916.1 1974268..1974705(-) (comGF/cglF) [Streptococcus cristatus AS 1.3089]
GTGTTAAAAAACGTTAAAATCAAAGCTTTTACACTTTTAGAAACGCTAGTTGCTCTGCTGGTCTTGAGTGGTAGTATGCT
AGTCTTTCAGTCGCTGACCAAGCTTCTGGCTTTTGAACTGCGACAGCAACAGGAAAATAAGCAGCAGGAATGGCTCTTAT
TTGTCGATCAGTTAGAGACAGAGCTATCGCGCAGCCAATTTGACAGGGTCGAAAACGACAAAATCTACGTCAAACAGGAT
GGCAAAAATATAGCTTTAGGAAAATCTAGGAGTGATGATTTCCGCAAGACGGATGCTTCGGGACGTGGCTATCAGCCCAT
GGTTTATGGGCTCAAGGCTGCTCCGATTACTCAAAAGGGGAATCTTGTCCGCTTTCGTTTCCAGTTTGAAAATGATTTAG
AAAGAGAGTACATTTATCGTGTCCAAGATAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(39-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

70

96.552

0.676
  comGF/cglF Streptococcus mitis SK321

67.143

96.552

0.648
  comGF/cglF Streptococcus pneumoniae Rx1

65

96.552

0.628
  comGF/cglF Streptococcus pneumoniae D39

65

96.552

0.628
  comGF/cglF Streptococcus pneumoniae R6

65

96.552

0.628
  comGF/cglF Streptococcus pneumoniae TIGR4

65

96.552

0.628
  comGF Lactococcus lactis subsp. cremoris KW2

43.885

95.862

0.421
  comYF Streptococcus mutans UA140

41.379

100

0.414
  comYF Streptococcus mutans UA159

40.69

100

0.407

Multiple sequence alignment