Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L39_RS03955 Genome accession   NZ_CP077395
Coordinates   838565..840271 (+) Length   568 a.a.
NCBI ID   WP_042077628.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1409     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 833565..845271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L39_RS03930 (I6L39_03930) pdhR 834695..835459 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  I6L39_RS03935 (I6L39_03935) ampD 835738..836307 (-) 570 WP_216991093.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6L39_RS03940 (I6L39_03940) - 836420..836890 (+) 471 WP_042077632.1 TIGR02281 family clan AA aspartic protease -
  I6L39_RS03945 (I6L39_03945) nadC 836894..837769 (+) 876 WP_216991094.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6L39_RS03950 (I6L39_03950) tapA 838079..838561 (+) 483 WP_216991095.1 type IVa pilus major pilin TapA -
  I6L39_RS03955 (I6L39_03955) pilB 838565..840271 (+) 1707 WP_042077628.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6L39_RS03960 (I6L39_03960) pilC 840399..841640 (+) 1242 WP_209794187.1 type II secretion system F family protein Machinery gene
  I6L39_RS03965 (I6L39_03965) pilD 841708..842583 (+) 876 WP_042077625.1 A24 family peptidase Machinery gene
  I6L39_RS03970 (I6L39_03970) coaE 842602..843216 (+) 615 WP_042077623.1 dephospho-CoA kinase -
  I6L39_RS03975 (I6L39_03975) zapD 843254..843976 (+) 723 WP_042077622.1 cell division protein ZapD -
  I6L39_RS03980 (I6L39_03980) yacG 843989..844183 (+) 195 WP_042077620.1 DNA gyrase inhibitor YacG -
  I6L39_RS03985 (I6L39_03985) mutT 844242..844646 (-) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62633.01 Da        Isoelectric Point: 5.8214

>NTDB_id=581136 I6L39_RS03955 WP_042077628.1 838565..840271(+) (pilB) [Aeromonas sp. FDAARGOS 1409]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTNEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=581136 I6L39_RS03955 WP_042077628.1 838565..840271(+) (pilB) [Aeromonas sp. FDAARGOS 1409]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTTGAGTATGGGGTACCTCTGCTGGATCTGGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACGGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAATTGATGGAAAGCGAGCAGGATACCCTCGGCATGGATCACATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGACGAGAACTCTCGTCTCGATGAGAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGACCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAGATCGCCACCCCGCCGGTGAACCTGGCAAACCGTTTCGCGGCGCGC
TTAAAAGTCATGGCGCGGCTCGACATCGCCGAGCGGCGTCTGCCCCAGGATGGCCGGATCAAGCTCAAGATTTCGCGCAA
CAGATCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGTGAGAAGATCGTCATCCGGCTACTAGATTCATCTG
CAGCCAGGCTCAATATCGATCAACTCGGCTTTGACGACCGCCAGAAGGGGCAATACCTGCATGCCCTCTCCAAACCCCAG
GGGATGATACTGGTCACCGGCCCCACGGGGTCGGGCAAGACGGTGTCACTCTATACCGGTCTCAACATCCTCAATACCAA
CGAGGTCAACATCTCTACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTGAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAATTCGGCGGC
CGAGACGCTCACCCGGATGATGAACATGGGTGTCCCCGCCTTCAACATCGCCTCCTCGGTCACCCTCATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCGCCCGAGGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAACTGGCCGCCGGCTTGCGACTGTTCAAGCCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGAGGACGGGT
CGGGATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATCGCGG
CCATCGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCCGGCCTTGAAAAAGCCCGCCTGGGAGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.681

97.183

0.366