Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L45_RS08785 Genome accession   NZ_CP077363
Coordinates   1836270..1837976 (-) Length   568 a.a.
NCBI ID   WP_216983982.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1415     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831270..1842976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L45_RS08755 (I6L45_08755) mutT 1831894..1832298 (+) 405 WP_216983978.1 8-oxo-dGTP diphosphatase MutT -
  I6L45_RS08760 (I6L45_08760) yacG 1832357..1832551 (-) 195 WP_043762810.1 DNA gyrase inhibitor YacG -
  I6L45_RS08765 (I6L45_08765) zapD 1832562..1833284 (-) 723 WP_167576542.1 cell division protein ZapD -
  I6L45_RS08770 (I6L45_08770) coaE 1833322..1833936 (-) 615 WP_216983979.1 dephospho-CoA kinase -
  I6L45_RS08775 (I6L45_08775) pilD 1833955..1834827 (-) 873 WP_216983980.1 A24 family peptidase Machinery gene
  I6L45_RS08780 (I6L45_08780) pilC 1834904..1836145 (-) 1242 WP_216983981.1 type II secretion system F family protein Machinery gene
  I6L45_RS08785 (I6L45_08785) pilB 1836270..1837976 (-) 1707 WP_216983982.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6L45_RS08790 (I6L45_08790) - 1837983..1838411 (-) 429 WP_216983983.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I6L45_RS08795 (I6L45_08795) nadC 1838719..1839582 (-) 864 WP_216983984.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6L45_RS08800 (I6L45_08800) - 1839586..1840056 (-) 471 WP_202016401.1 TIGR02281 family clan AA aspartic protease -
  I6L45_RS08805 (I6L45_08805) ampD 1840186..1840758 (+) 573 WP_216983985.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6L45_RS08810 (I6L45_08810) pdhR 1841035..1841799 (+) 765 WP_043762834.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62593.93 Da        Isoelectric Point: 5.8214

>NTDB_id=580897 I6L45_RS08785 WP_216983982.1 1836270..1837976(-) (pilB) [Aeromonas sp. FDAARGOS 1415]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTTAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRIDEGVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPETVPEAELLELGFS
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=580897 I6L45_RS08785 WP_216983982.1 1836270..1837976(-) (pilB) [Aeromonas sp. FDAARGOS 1415]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGTCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAACGCTA
TCTGAGCCAGGCCAGGGCACAGCGCAAGCCCTTTGTCACCTTCCTCATTGAAAACGACATCCTCGACAGCAAGGCCCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATTTAGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATTGCCATGTCGGA
TCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCTTGCACACCGAGGCCCTGCTGGTCGAGGAGAGCA
AGCTGACCACCGCCATAGGCAAGCTGATGGAGAGCGAGCAGGATACCCTTGGCATGGACCACATCGATGAGGCCGAGATC
TCTGAGCTCGAGGTTTCCGATGAGAGCTCTCGCATCGACGAGGGCGTCAACACTGCCGACGATGATGCTCCCATCGTCAA
GTACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCATCGGACCTGCATTTCGAGCCTTATGAGCAGAAGTACC
GGATCCGGTTTCGCATTGACGGCATACTGCACGAGATCGCCACCCCGCCGGTCAATCTGGCCAACCGCTTCGCGGCCCGC
CTCAAGGTGATGGCCCGCCTCGATATCGCCGAACGGCGCCTGCCCCAGGACGGCCGGATCAAGCTCAAAATTTCGCGCAA
CCGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGGCTGCTGGACTCGTCGG
CCGCGCGCCTCAACATCGATCAGCTCGGCTTCGACGACCGCCAGAAGGGACAATATCTTCACGCCCTCTCCAAGCCACAG
GGCATGATACTGGTTACCGGCCCCACAGGGTCGGGCAAGACAGTGTCGCTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAGGATCCGGTGGAAATCAACCTGCCTGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCCTGCGTCAGGACCCCGACATCGTCATGGTAGGCGAAATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCTACTCTGCACACCAATTCGGCGGC
CGAGACACTCACCCGCATGATGAACATGGGCGTCCCCGCCTTCAACATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GTCTCGCCCGCAAACTGTGCGACCACTGCAAGGCCCCCGAAACAGTACCGGAAGCCGAGCTGCTGGAGCTCGGATTTAGC
CAGCAGCAGCTCGCTGCAGGCTTGCGCCTGTTCAAGCCCGTGGGTTGCAAAGAGTGTTCGGGAGGATACAGAGGACGCGT
CGGCATCTATGAGATCATGCTGATGTCGGACAATATCGCCAAGCTCATCATGCAGGGAGCCAACTCGCTGCAAATCGCGG
CCATCGCGCAGAAGGAAGGGATGCGTACCCTGCGGACCTCGGGCCTGGAAAAAGCCCGTCTTGGGGTCACCAGCCTGGCC
GAAATAAACCGTATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.674

99.824

0.546
  pilB Acinetobacter baylyi ADP1

54.707

99.12

0.542
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

52.715

100

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

50.266

99.12

0.498
  pilB Vibrio campbellii strain DS40M4

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.862

97.183

0.368