Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L33_RS18300 Genome accession   NZ_CP077249
Coordinates   3994712..3995851 (+) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3989712..4000851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L33_RS18270 (I6L33_18270) - 3989894..3990274 (-) 381 WP_005302618.1 diacylglycerol kinase -
  I6L33_RS18275 (I6L33_18275) - 3990431..3991492 (+) 1062 WP_017766699.1 3-deoxy-7-phosphoheptulonate synthase -
  I6L33_RS18280 (I6L33_18280) - 3991572..3992357 (-) 786 WP_216973068.1 ATP-binding cassette domain-containing protein -
  I6L33_RS18285 (I6L33_18285) btuC 3992344..3993351 (-) 1008 WP_017785953.1 vitamin B12 ABC transporter permease BtuC -
  I6L33_RS18290 (I6L33_18290) - 3993451..3994182 (-) 732 WP_025201472.1 ABC transporter substrate-binding protein -
  I6L33_RS18295 (I6L33_18295) - 3994296..3994652 (+) 357 WP_005302630.1 DMT family protein -
  I6L33_RS18300 (I6L33_18300) pilU 3994712..3995851 (+) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  I6L33_RS18305 (I6L33_18305) glgC 3996110..3997381 (+) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  I6L33_RS18310 (I6L33_18310) - 3997517..3997996 (+) 480 WP_216973069.1 WbuC family cupin fold metalloprotein -
  I6L33_RS18315 (I6L33_18315) - 3998120..3998362 (-) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  I6L33_RS18320 (I6L33_18320) feoB 3998359..4000632 (-) 2274 WP_216973070.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=580321 I6L33_RS18300 WP_017766703.1 3994712..3995851(+) (pilU) [Aeromonas sp. FDAARGOS 1403]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=580321 I6L33_RS18300 WP_017766703.1 3994712..3995851(+) (pilU) [Aeromonas sp. FDAARGOS 1403]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCGCGCAACATCAAGACCGAGATCCCTCGCTTCGA
GGACTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTCGCAGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGGCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGACGAGGAGGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAACCCACCAAGGACG
AAAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361