Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SPN034156_RS04965 Genome accession   NC_021006
Coordinates   907120..908136 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae SPN034156     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 902120..913136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN034156_RS13030 - 902197..902328 (-) 132 WP_000768904.1 hypothetical protein -
  SPN034156_RS04920 (SPN034156_08810) - 902329..903519 (-) 1191 WP_000167765.1 acetate kinase -
  SPN034156_RS04925 (SPN034156_08820) comYH 903570..904523 (-) 954 WP_000345099.1 class I SAM-dependent methyltransferase Machinery gene
  SPN034156_RS11320 (SPN034156_08830) - 904584..905178 (-) 595 Protein_911 methyltransferase domain-containing protein -
  SPN034156_RS04940 (SPN034156_08840) comGG/cglG 905315..905728 (-) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPN034156_RS04945 (SPN034156_08850) comGF/cglF 905706..906167 (-) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPN034156_RS04950 (SPN034156_08860) comGE/cglE 906130..906432 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPN034156_RS04955 (SPN034156_08870) comGD/cglD 906395..906799 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPN034156_RS04960 (SPN034156_08880) comGC/cglC 906792..907118 (-) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  SPN034156_RS04965 (SPN034156_08890) comGB/cglB 907120..908136 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPN034156_RS04970 (SPN034156_08900) comGA/cglA/cilD 908084..909025 (-) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  SPN034156_RS04975 (SPN034156_08910) - 909101..909466 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPN034156_RS04980 (SPN034156_08920) - 909617..910675 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SPN034156_RS04985 (SPN034156_08930) nagA 910838..911989 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=58030 SPN034156_RS04965 WP_074017570.1 907120..908136(-) (comGB/cglB) [Streptococcus pneumoniae SPN034156]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=58030 SPN034156_RS04965 WP_074017570.1 907120..908136(-) (comGB/cglB) [Streptococcus pneumoniae SPN034156]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment