Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L27_RS13265 Genome accession   NZ_CP077232
Coordinates   2818654..2820366 (+) Length   570 a.a.
NCBI ID   WP_216967409.1    Uniprot ID   -
Organism   Acinetobacter pittii strain FDAARGOS 1397     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2813654..2825366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L27_RS13230 (I6L27_13235) nusA 2814542..2816026 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  I6L27_RS13235 (I6L27_13240) rimP 2816064..2816588 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  I6L27_RS13255 (I6L27_13260) secG 2817226..2817555 (-) 330 WP_016142373.1 preprotein translocase subunit SecG -
  I6L27_RS13260 (I6L27_13265) tpiA 2817568..2818362 (-) 795 WP_031947213.1 triose-phosphate isomerase -
  I6L27_RS13265 (I6L27_13270) pilB 2818654..2820366 (+) 1713 WP_216967409.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6L27_RS13270 (I6L27_13275) pilC 2820394..2821620 (+) 1227 WP_057104884.1 type II secretion system F family protein Machinery gene
  I6L27_RS13275 (I6L27_13280) pilD 2821620..2822480 (+) 861 WP_125699029.1 prepilin peptidase Machinery gene
  I6L27_RS13280 (I6L27_13285) coaE 2822482..2823078 (+) 597 WP_062849621.1 dephospho-CoA kinase -
  I6L27_RS13285 (I6L27_13290) - 2823075..2823989 (-) 915 WP_062849622.1 DMT family transporter -
  I6L27_RS13290 (I6L27_13295) rlmB 2824025..2824774 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6L27_RS13295 (I6L27_13300) - 2824877..2825203 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63627.06 Da        Isoelectric Point: 5.0932

>NTDB_id=580074 I6L27_RS13265 WP_216967409.1 2818654..2820366(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1397]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=580074 I6L27_RS13265 WP_216967409.1 2818654..2820366(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1397]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAAATT
CCTAAAGATTTGGTTGACCAAAAACTGATTTTAAAACATCGGATCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGTAAACTTATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCACCACCATCACAAGAGGATGAGGAAGAAACACCCCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATAGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATCGATTTTCGCGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTAGATAAACCA
CAAGGCATGCTTTTAATTACAGGGCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAAGTTAACGTCAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGTAATATGGGCGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTAGTGATTGCTC
AACGTTTAGCACGTCGGCTATGCACTCAGTGTAAAATACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTCGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.614

100

0.956
  pilB Acinetobacter baylyi ADP1

78.421

100

0.784
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.386
  pilF Thermus thermophilus HB27

36.833

98.596

0.363