Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L47_RS11460 Genome accession   NZ_CP077221
Coordinates   2536713..2538419 (+) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1417     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531713..2543419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L47_RS11435 (I6L47_11435) pdhR 2532804..2533568 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  I6L47_RS11440 (I6L47_11440) ampD 2533847..2534422 (-) 576 WP_139440264.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6L47_RS11445 (I6L47_11445) - 2534553..2535023 (+) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  I6L47_RS11450 (I6L47_11450) nadC 2535027..2535902 (+) 876 WP_216947458.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6L47_RS11455 (I6L47_11455) tapA 2536230..2536709 (+) 480 WP_216949346.1 type IVa pilus major pilin TapA -
  I6L47_RS11460 (I6L47_11460) pilB 2536713..2538419 (+) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6L47_RS11465 (I6L47_11465) pilC 2538659..2539900 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  I6L47_RS11470 (I6L47_11470) pilD 2539962..2540834 (+) 873 WP_216947459.1 A24 family peptidase Machinery gene
  I6L47_RS11475 (I6L47_11475) coaE 2540848..2541462 (+) 615 WP_216947460.1 dephospho-CoA kinase -
  I6L47_RS11480 (I6L47_11480) zapD 2541500..2542222 (+) 723 WP_216947461.1 cell division protein ZapD -
  I6L47_RS11485 (I6L47_11485) yacG 2542232..2542426 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  I6L47_RS11490 (I6L47_11490) mutT 2542504..2542908 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=579947 I6L47_RS11460 WP_005336134.1 2536713..2538419(+) (pilB) [Aeromonas sp. FDAARGOS 1417]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=579947 I6L47_RS11460 WP_005336134.1 2536713..2538419(+) (pilB) [Aeromonas sp. FDAARGOS 1417]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCTCTGATAAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCTATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACTAATGTCTCGGCATTGGAAGACTTTGGCTTCAGCTTCGGTCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGCCGACTGGACGAGAGTGTCAATACCGCTGATGACGATGCTCCCATTGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAAATCGCCACACCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTAATGGCGCGGCTCGATATTGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTAAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTCATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCGCAG
GGGATGATCCTAGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACTAC
CGAGGTAAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTTAACCAAGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCTTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCAGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGA
GGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCGGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATAGCCAAATTGATCATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCCCAAAAAGAGGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377