Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L49_RS17895 Genome accession   NZ_CP077209
Coordinates   3909295..3911001 (-) Length   568 a.a.
NCBI ID   WP_005356616.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1419     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3904295..3916001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L49_RS17865 (I6L49_17865) mutT 3904805..3905209 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  I6L49_RS17870 (I6L49_17870) yacG 3905287..3905481 (-) 195 WP_216950045.1 DNA gyrase inhibitor YacG -
  I6L49_RS17875 (I6L49_17875) zapD 3905491..3906213 (-) 723 WP_005336141.1 cell division protein ZapD -
  I6L49_RS17880 (I6L49_17880) coaE 3906251..3906865 (-) 615 WP_019444785.1 dephospho-CoA kinase -
  I6L49_RS17885 (I6L49_17885) pilD 3906879..3907751 (-) 873 WP_216950046.1 A24 family peptidase Machinery gene
  I6L49_RS17890 (I6L49_17890) pilC 3907813..3909054 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  I6L49_RS17895 (I6L49_17895) pilB 3909295..3911001 (-) 1707 WP_005356616.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6L49_RS17900 (I6L49_17900) - 3911004..3911447 (-) 444 WP_216950047.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I6L49_RS17905 (I6L49_17905) nadC 3911763..3912626 (-) 864 WP_216950048.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6L49_RS17910 (I6L49_17910) - 3912630..3913100 (-) 471 WP_216950049.1 TIGR02281 family clan AA aspartic protease -
  I6L49_RS17915 (I6L49_17915) ampD 3913231..3913806 (+) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6L49_RS17920 (I6L49_17920) pdhR 3914085..3914849 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62563.90 Da        Isoelectric Point: 5.3192

>NTDB_id=579851 I6L49_RS17895 WP_005356616.1 3909295..3911001(-) (pilB) [Aeromonas sp. FDAARGOS 1419]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=579851 I6L49_RS17895 WP_005356616.1 3909295..3911001(-) (pilB) [Aeromonas sp. FDAARGOS 1419]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCGCTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCATTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAGAGCGTCAATACCACCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCAGACCTGCACTTCGAGCCTTATGAGTTCAAGTATC
GGGTTCGCTTTCGCATAGATGGCATTCTGCATGAAATTGCCACACCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGTTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCGAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCTGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAATCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCACAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGGCTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATTATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATAGCGCAGAAAGAGGGGATGCGTACCCTGCGTACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377