Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6L28_RS01400 Genome accession   NZ_CP077205
Coordinates   299044..300756 (+) Length   570 a.a.
NCBI ID   WP_216946731.1    Uniprot ID   -
Organism   Acinetobacter pittii strain FDAARGOS 1398     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294044..305756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L28_RS01365 (I6L28_01365) nusA 294932..296416 (-) 1485 WP_216946729.1 transcription termination factor NusA -
  I6L28_RS01370 (I6L28_01370) rimP 296454..296978 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  I6L28_RS01390 (I6L28_01390) secG 297616..297945 (-) 330 WP_016142373.1 preprotein translocase subunit SecG -
  I6L28_RS01395 (I6L28_01395) tpiA 297958..298752 (-) 795 WP_216946730.1 triose-phosphate isomerase -
  I6L28_RS01400 (I6L28_01400) pilB 299044..300756 (+) 1713 WP_216946731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6L28_RS01405 (I6L28_01405) pilC 300784..302010 (+) 1227 WP_216946732.1 type II secretion system F family protein Machinery gene
  I6L28_RS18165 (I6L28_01410) pilD 302010..302870 (+) 861 WP_016144165.1 prepilin peptidase Machinery gene
  I6L28_RS01415 (I6L28_01415) coaE 302872..303468 (+) 597 WP_216946733.1 dephospho-CoA kinase -
  I6L28_RS01420 (I6L28_01420) - 303465..304379 (-) 915 WP_216946734.1 DMT family transporter -
  I6L28_RS01425 (I6L28_01425) rlmB 304415..305164 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6L28_RS01430 (I6L28_01430) - 305267..305593 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63671.07 Da        Isoelectric Point: 5.0932

>NTDB_id=579764 I6L28_RS01400 WP_216946731.1 299044..300756(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1398]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLRLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSATLKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=579764 I6L28_RS01400 WP_216946731.1 299044..300756(+) (pilB) [Acinetobacter pittii strain FDAARGOS 1398]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAAATT
CCTAAAGATTTGGTTGACCAAAAACTGATTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCACCACCATCACAAGAGGATGAGGAAGAAACACCCCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATAGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTACAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAGGCTCTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTAGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAGCCC
CAAGGCATGCTTTTAATTACAGGGCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAATGTCAACCCGA
AAGTAGGTCTGACTTTTTCGGCCACACTGAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTCATGTCGACACTGCATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGTAATATGGGCGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTGGTGATTGCTC
AACGTTTAGCACGTCGGCTATGCACTCAGTGTAAAATACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTTCAACCTGTCGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAGGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.088

100

0.951
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

50.943

92.982

0.474
  pilB Vibrio campbellii strain DS40M4

50.383

91.579

0.461
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.993

100

0.388
  pilF Thermus thermophilus HB27

36.833

98.596

0.363