Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU722_RS02965 Genome accession   NZ_CP077084
Coordinates   687340..688665 (+) Length   441 a.a.
NCBI ID   WP_065875727.1    Uniprot ID   A0A8I0CV40
Organism   Pseudomonas tritici strain SWRI145     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 682340..693665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU722_RS02945 (HU722_0002945) - 682430..684892 (-) 2463 WP_065889146.1 penicillin-binding protein 1A -
  HU722_RS02950 (HU722_0002950) pilM 685110..685763 (+) 654 WP_065875730.1 type IV pilus biogenesis protein PilM -
  HU722_RS02955 (HU722_0002955) - 685760..686314 (+) 555 WP_065875729.1 PilN domain-containing protein -
  HU722_RS02960 (HU722_0002960) - 686311..687270 (+) 960 WP_065875728.1 pilus assembly protein PilP -
  HU722_RS02965 (HU722_0002965) pilQ 687340..688665 (+) 1326 WP_065875727.1 type IV pilus secretin PilQ Machinery gene
  HU722_RS02970 (HU722_0002970) aroK 688670..689188 (+) 519 WP_010213757.1 shikimate kinase AroK -
  HU722_RS02975 (HU722_0002975) aroB 689314..690414 (+) 1101 WP_065875726.1 3-dehydroquinate synthase -
  HU722_RS02980 (HU722_0002980) - 690425..691981 (+) 1557 WP_049710402.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47838.65 Da        Isoelectric Point: 4.7681

>NTDB_id=578906 HU722_RS02965 WP_065875727.1 687340..688665(+) (pilQ) [Pseudomonas tritici strain SWRI145]
MATAVPNRVDLIQLPPPGSATSSAYTGDKLNLNFQSIELRTALQQIADVAGLNLVASDDVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLNRGNWGAGGIPKPQSEGAEPPE
NPPSSPFVDLGSLTGTSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=578906 HU722_RS02965 WP_065875727.1 687340..688665(+) (pilQ) [Pseudomonas tritici strain SWRI145]
ATGGCTACTGCTGTGCCCAATCGTGTGGATCTGATCCAGTTGCCGCCTCCCGGCAGCGCCACGTCAAGCGCCTACACAGG
CGACAAGCTGAACCTTAACTTCCAGAGCATCGAGTTGCGTACCGCGTTGCAGCAAATTGCCGATGTGGCAGGCCTCAACC
TGGTAGCGAGCGACGACGTGCAGGGCTCGATCACGCTGCGCCTCAAGGATGTGCCCTGGGACCAGGCGCTGGACCTGGTA
TTGCAAGCCAAGGGATTGGATAAGCGGGTAAAGGCCGGCGTATTGCTGGTGGCGCCTGCCGAGGAGTTGGCCGCGCGTGA
GCTGCTGACCCTTGAGTCGCGCAAGCAAATGGCCGAACTGGCGCCGTTGCGCCGTGAGCTGTTGCAAGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGCTGTTCCAATCGGTCACGGGCCTCGAGGGCGTCACCGATGAGCGAGGCTCGGTGGCGGTC
GATGATCGCACCAACAACATCATTGCCTACCAGACCGGGGAGCGGCTCGAGGAGCTGCGGCGGATCGTGGCTCAGCTGGA
TATTCCGGTGCGCCAGGTCATGATCGAGGCGCGGATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGCGCGCGCT
GGGGTGGCCGGCTGAATCGCGGAAACTGGGGCGCGGGGGGTATCCCCAAGCCTCAGTCTGAGGGCGCGGAACCACCAGAG
AACCCACCCAGCTCACCCTTTGTGGATTTGGGTTCGCTGACCGGCACCTCGGGCCTGGGCATCGCCTTTATCACCGATAA
CTTGCTGCTGGACCTGGAACTCACCGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAACCCAAAGTGGTCACCT
CCGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATTCCTTATCAGGAATCCAGTTCCAGTGGCGCCACTTCGGTG
TCGTTCAAGGAAGCGTCGCTGTCGCTGGAGGTCACCCCGCAAATCACCCCCGACGACCGCGTGATCATGGAGGTCAAGGT
CACCAAGGATGAGCCCGATTACCTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTGT
TGGTGAAGGATGGCGAGACCATTGTTATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.814

100

0.71
  comQ Acinetobacter baylyi ADP1

42.105

100

0.435
  pilQ Vibrio cholerae strain A1552

43.852

97.732

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.852

97.732

0.429
  pilQ Acinetobacter baumannii D1279779

40.517

100

0.426
  pilQ Acinetobacter baumannii strain A118

40.517

100

0.426
  pilQ Vibrio campbellii strain DS40M4

43.735

95.918

0.419
  pilQ Legionella pneumophila strain Lp02

43.468

95.465

0.415
  pilQ Legionella pneumophila strain ERS1305867

42.993

95.465

0.41