Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU759_RS02975 Genome accession   NZ_CP077082
Coordinates   677851..679179 (+) Length   442 a.a.
NCBI ID   WP_017138568.1    Uniprot ID   A0A7Y7V960
Organism   Pseudomonas sp. OE 28.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672851..684179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU759_RS02955 (HU759_002955) - 672897..675362 (-) 2466 WP_176991728.1 penicillin-binding protein 1A -
  HU759_RS02960 (HU759_002960) pilM 675579..676235 (+) 657 WP_099548046.1 type IV pilus assembly protein PilM -
  HU759_RS02965 (HU759_002965) - 676232..676783 (+) 552 WP_170039053.1 PilN domain-containing protein -
  HU759_RS02970 (HU759_002970) - 676780..677781 (+) 1002 WP_186618465.1 pilus assembly protein PilP -
  HU759_RS02975 (HU759_002975) pilQ 677851..679179 (+) 1329 WP_017138568.1 type IV pilus secretin PilQ family protein Machinery gene
  HU759_RS02980 (HU759_002980) aroK 679184..679702 (+) 519 WP_017138567.1 shikimate kinase AroK -
  HU759_RS02985 (HU759_002985) aroB 679826..680926 (+) 1101 WP_186618467.1 3-dehydroquinate synthase -
  HU759_RS02990 (HU759_002990) - 680937..682505 (+) 1569 WP_017138565.1 AAA family ATPase -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48170.14 Da        Isoelectric Point: 4.7681

>NTDB_id=578857 HU759_RS02975 WP_017138568.1 677851..679179(+) (pilQ) [Pseudomonas sp. OE 28.3]
MAFAVPNRVDLIQLPPPGSVVPSGAYNGDTLTLNFQNIELRAVLQQIADVAGLNLVASDEVQGSITLRLKDVPWDQALDL
VLQTKGLDKRVKAGVLLVAPAEELAARELLALESRKQMEELAPLRRELLQVNYAKAADLAKLFQSVTGVDGFTDERGSVA
VDDRTNNIIAYQTREQIDELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGISKPLAEGAEPP
ENPPSSPFVDMGSLAGTSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSRGATS
VSFKEASLSLEVTPQITPDGWVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVRVKDGETIVIGGVFSNTQSKVVDKVP
FLGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=578857 HU759_RS02975 WP_017138568.1 677851..679179(+) (pilQ) [Pseudomonas sp. OE 28.3]
ATGGCATTTGCTGTGCCCAATCGCGTCGATCTGATCCAGCTGCCGCCTCCTGGCAGCGTGGTTCCCAGTGGCGCCTACAA
CGGCGACACGCTGACCCTCAACTTCCAGAACATCGAATTGCGCGCCGTGTTGCAGCAAATTGCGGATGTGGCCGGGCTTA
ATCTGGTGGCCAGTGATGAAGTGCAAGGCTCGATCACTTTGCGGCTCAAGGATGTGCCCTGGGACCAGGCGCTGGATCTG
GTGCTGCAAACCAAAGGCTTGGATAAGCGCGTGAAGGCCGGCGTTTTGCTGGTGGCACCCGCCGAAGAATTGGCGGCCCG
CGAGCTGCTGGCGCTGGAGTCGCGCAAGCAGATGGAAGAGCTGGCGCCCTTGCGGCGCGAGCTGTTGCAGGTCAATTACG
CCAAGGCGGCGGACCTGGCCAAGTTGTTCCAGTCGGTGACAGGCGTTGACGGCTTTACCGATGAGCGCGGCTCGGTGGCG
GTGGATGATCGCACCAATAACATCATCGCCTACCAGACCCGCGAGCAAATTGACGAGCTGCGGCGCATCGTGGCGCAGCT
GGATGTACCGGTACGCCAAGTGATGATCGAGGCGCGGATTGTCGAGGCTAATGTCGATTACGACAAAAGCCTGGGCGTGC
GCTGGGGCGGGCGGCTCAATCGCGGCAACTGGGGCGCGGGCGGTATCAGCAAGCCGTTGGCCGAGGGTGCGGAGCCACCG
GAGAATCCGCCCAGCTCGCCGTTTGTCGATATGGGCTCGCTGGCCGGTACGTCTGGCTTGGGCATCGCTTTTATTACCGA
CAACCTGTTGCTCGACCTGGAACTGACGGCCATGGAAAAAACCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCA
CTTCCGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATTCCTTACCAGGAGTCCAGCTCGCGTGGCGCCACCTCA
GTGTCGTTCAAGGAGGCCTCGCTGTCGTTGGAGGTGACCCCGCAAATCACCCCGGATGGCTGGGTGATTATGGAGGTCAA
GGTTACCAAGGACGAGCCGGACTACCTGAACAAGCTCAACGACGTACCGCCGATCAAGAAAAACGAAGTCAACGCCAAGG
TGCGGGTCAAGGATGGCGAGACCATCGTCATTGGCGGGGTTTTCTCCAATACGCAAAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTACCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(277-433)

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7V960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.252

98.869

0.695
  pilQ Acinetobacter baumannii strain A118

40.891

100

0.457
  pilQ Acinetobacter baumannii D1279779

40.891

100

0.457
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.289

97.059

0.43
  pilQ Vibrio cholerae strain A1552

44.289

97.059

0.43
  comQ Acinetobacter baylyi ADP1

42.094

100

0.428
  pilQ Vibrio campbellii strain DS40M4

44.444

95.701

0.425
  pilQ Legionella pneumophila strain Lp02

43.571

95.023

0.414
  pilQ Legionella pneumophila strain ERS1305867

43.571

95.023

0.414