Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KMZ14_RS01280 Genome accession   NZ_CP076603
Coordinates   268263..269972 (-) Length   569 a.a.
NCBI ID   WP_273798378.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain MCDA01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 263263..274972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ14_RS01250 (KMZ14_01250) - 263375..263691 (+) 317 Protein_237 pyrimidine/purine nucleoside phosphorylase -
  KMZ14_RS01255 (KMZ14_01255) rlmB 263844..264593 (+) 750 WP_004810085.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KMZ14_RS01260 (KMZ14_01260) - 264600..265523 (+) 924 WP_081405839.1 DMT family transporter -
  KMZ14_RS01265 (KMZ14_01265) coaE 265544..266140 (-) 597 WP_273798376.1 dephospho-CoA kinase -
  KMZ14_RS01270 (KMZ14_01270) pilD 266142..267002 (-) 861 WP_273798377.1 prepilin peptidase Machinery gene
  KMZ14_RS01275 (KMZ14_01275) pilC 267002..268231 (-) 1230 WP_004810078.1 type II secretion system F family protein Machinery gene
  KMZ14_RS01280 (KMZ14_01280) pilB 268263..269972 (-) 1710 WP_273798378.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KMZ14_RS01285 (KMZ14_01285) tpiA 270255..271046 (+) 792 WP_163164692.1 triose-phosphate isomerase -
  KMZ14_RS01290 (KMZ14_01290) secG 271060..271389 (+) 330 WP_273798379.1 preprotein translocase subunit SecG -
  KMZ14_RS01320 (KMZ14_01320) rimP 272450..272974 (+) 525 WP_004810072.1 ribosome maturation factor RimP -
  KMZ14_RS01325 (KMZ14_01325) nusA 273010..274494 (+) 1485 WP_004810069.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63495.65 Da        Isoelectric Point: 4.8758

>NTDB_id=576579 KMZ14_RS01280 WP_273798378.1 268263..269972(-) (pilB) [Acinetobacter schindleri strain MCDA01]
MTAIQGSPRFTGFVRRLVDEGVISAEDMQGALSSAKKDKVDIVPYLIEHHRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNSNILFIATSNPTNIEAIDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDIDSSDPNKDDENDDKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKPADIPRQSLLEMGFS
DDDLQNPEFNIFEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=576579 KMZ14_RS01280 WP_273798378.1 268263..269972(-) (pilB) [Acinetobacter schindleri strain MCDA01]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTGTTCGTCGACTTGTCGATGAAGGGGTTATATCTGCAGAAGA
TATGCAGGGTGCTTTGAGCAGTGCCAAAAAAGATAAAGTTGATATTGTTCCCTATCTGATTGAACATCACCGCCTCTCGC
CAACCAAAATCGCAGAAACGATTTCTCTTGAATTCGGTGAACCGCTATTTGATATCAGTGCTTATGATCCTGCCCTGATC
GTACGCGAAGATTTTGATGAAAAGATTATTACCAAACACCGCGTGCTGCCGATCTTTAAAAATAGCAATATTCTGTTTAT
CGCGACCAGTAACCCGACCAATATCGAAGCAATTGATGCTGTTCGTTTTGCGACCAAACTCAATATTGAAACCATCATTG
TTGAACATAATAAACTCGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGACTTTGATGATGATTTTGAT
CTAGATGTTGATATCGATAGCTCTGACCCGAATAAAGATGATGAGAATGACGATAAAGGTGATGAAGCACCCATTGTTAA
ATATATTAATAAGTTACTGATTGACGCAATCCGTATGGGTGCATCTGATTTACACTTTGAACCTTATGAAAAAATGTACC
GTGTGCGTTACCGTGTCGACGGGGTCTTGCGTCAAATCGCCACGCCACCTTTACAGCTTGCAACACGTTTGGCTTCACGT
CTTAAAGTTATGTCACAAATGGATATTTCCGAGAAGCGTGTGCCTCAAGATGGCCGGATCAAGTTAAAACTCTCTAAAAA
TAAGGCCATCGATTTCCGTGTCAACTCACTCCCCACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATCCATCGA
GTGCCATGCTTGGAATTGATGCTTTGGGGTATGAGCCTGATCAAAAAGCCTTATTTATGGAGGCACTAGATAAGCCTCAA
GGTATGCTCTTGATCACCGGTCCGACAGGCTCTGGTAAAACTGTATCTCTCTATACCGGTTTAAATATCCTAAATCGTGA
AGACACGAATATTTCAACTGCTGAAGATCCAGTCGAAATTAACCTGCAAGGGATCAACCAGGTTAACGTCAATAATAAAG
TCGGCCTAACCTTCTCTGCTGCCTTGAAATCATTCCTGCGTCAGGACCCTGATATTGTCATGGTCGGTGAGATTCGTGAC
CTAGAAACTGCAGAGATTGCGATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACACTACACACCAACAGTGCACC
AGAAACCCTGACCCGTTTACGTAATATGGGTGTGCCATCATTTAATATTGCCACCTCGGTGAATTTGGTGATTGCTCAGC
GTTTGGCGCGTCGCCTTTGTCCCCATTGCAAAAAACCGGCAGATATTCCACGTCAGAGCCTGCTTGAAATGGGCTTTAGC
GATGATGATTTACAGAATCCGGAATTTAATATTTTTGAACCTGTCGGATGTAACGAATGTCGTGAAGGGTATAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCAGAGATTTCACGTATTATTATGGAAGATGGCAATGCAATTGAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.316

100

0.765
  pilB Legionella pneumophila strain ERS1305867

51.852

94.903

0.492
  pilF Neisseria gonorrhoeae MS11

49.376

98.594

0.487
  pilB Vibrio cholerae strain A1552

48.325

99.649

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.192

97.891

0.482
  pilB Vibrio campbellii strain DS40M4

47.935

97.891

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

34.528

100

0.373
  pilF Thermus thermophilus HB27

37.083

100

0.371