Detailed information NTG0057629

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: TOL_RS03350
• Genome accession: NC_020888
• Coordinates: 728610..729839 (-)
• Length: 409 a.a.
• NCBI ID: WP_015485865.1
• Uniprot ID: M5DNV2
• Organism: Thalassolituus oleivorans MIL-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 723610..734839

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TOL_RS03330 (TOL_0686)	-	725611..726792 (+)	1182	WP_015485861.1	response regulator	-
TOL_RS03335 (TOL_0687)	yacG	726848..727042 (-)	195	WP_015485862.1	DNA gyrase inhibitor YacG	-
TOL_RS03340 (TOL_0688)	coaE	727079..727678 (-)	600	WP_015485863.1	dephospho-CoA kinase	-
TOL_RS03345 (TOL_0689)	-	727718..728605 (-)	888	WP_015485864.1	prepilin peptidase	-
TOL_RS03350 (TOL_0690)	pilC	728610..729839 (-)	1230	WP_015485865.1	type II secretion system F family protein	Machinery gene
TOL_RS03355 (TOL_0691)	pilB	729884..731581 (-)	1698	WP_015485866.1	type IV-A pilus assembly ATPase PilB	Machinery gene
TOL_RS19385	-	731819..732313 (-)	495	WP_015485867.1	pilin	-
TOL_RS03365 (TOL_0693)	nadC	732593..733444 (-)	852	WP_015485868.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 409 a.a.
• Molecular weight: 44744.40 Da
• Isoelectric Point: 8.5748

>NTDB_id=57629 TOL_RS03350 WP_015485865.1 728610..729839(-) (pilC) [Thalassolituus oleivorans MIL-1]
MAKPIKTTTFQWEGTNRRGQTVKGELSGQNPAMVKAQLRKQGVEPKKIKRLSQPLFGLGGGVTKKRIKPADITFFTRQMA
TMVKAGVPLVQSFDIVADGVDNISLREVIYAIRDSVSAGNDFASALKQYPQYFDELTCNLIESGEQAGALESMLDKVAIY
KEKTEELKAKIKKALMYPMITLLVAFGVTAILLIKVVPTFDEMFKSFGAELPAPTQFIVMLSDWMQANYLYVFGGLIFLV
VAFMQGMKKSQSFRDNVETTLLKAPVFGDLIMKASVARYARVLSTTFAAGVPLVEALDSVAGAVGNVVYRNAVYNIRDEV
ASGQQMHFAMRATKVFPNMVVQMTSIGEESGALDSMLDKAASYYEAEVDNAVDNMTSLLEPAIMVFLGVVIGGMIVAMYL
PIFAMGDAI

Nucleotide

• Length: 1230 bp

>NTDB_id=57629 TOL_RS03350 WP_015485865.1 728610..729839(-) (pilC) [Thalassolituus oleivorans MIL-1]
ATGGCTAAACCAATAAAAACAACGACTTTCCAATGGGAAGGCACGAATCGCCGCGGACAAACCGTCAAGGGTGAGCTGAG
CGGTCAAAACCCAGCAATGGTAAAAGCACAGCTGCGCAAGCAAGGTGTTGAACCGAAAAAAATTAAACGCCTATCCCAAC
CTTTATTTGGTTTAGGCGGCGGAGTAACAAAGAAACGTATCAAACCTGCCGACATCACCTTTTTCACACGCCAAATGGCC
ACCATGGTGAAAGCGGGTGTACCTTTAGTACAGTCGTTCGATATTGTTGCCGACGGCGTGGATAATATTTCATTAAGAGA
AGTTATCTATGCCATTCGTGATTCGGTATCTGCCGGTAATGACTTTGCCTCGGCGTTAAAACAATATCCGCAGTATTTTG
ATGAGCTAACCTGCAACCTGATCGAATCGGGCGAGCAGGCGGGTGCGCTAGAGTCCATGCTGGATAAAGTCGCTATTTAC
AAAGAAAAAACCGAAGAACTGAAAGCTAAAATCAAAAAAGCGCTTATGTACCCTATGATCACCTTGTTGGTGGCATTTGG
GGTAACCGCTATCCTGTTAATCAAAGTCGTACCGACCTTTGATGAGATGTTTAAAAGCTTTGGCGCTGAATTACCCGCAC
CAACCCAGTTTATTGTGATGCTTTCCGATTGGATGCAAGCAAATTACCTATATGTCTTTGGTGGGCTAATTTTCCTAGTC
GTTGCTTTTATGCAAGGGATGAAGAAATCACAGTCGTTTCGAGATAATGTAGAAACAACATTACTCAAGGCTCCTGTTTT
CGGCGACTTGATAATGAAAGCATCGGTTGCACGGTATGCACGAGTACTGTCAACCACCTTCGCGGCGGGTGTTCCCTTGG
TTGAAGCCTTAGATTCCGTTGCAGGTGCCGTTGGCAACGTTGTATACCGCAATGCCGTATACAATATCCGTGATGAGGTC
GCCTCTGGTCAGCAAATGCATTTCGCTATGCGCGCGACTAAGGTATTCCCGAACATGGTGGTACAAATGACCAGCATTGG
TGAAGAGTCGGGTGCCCTTGATTCTATGCTAGATAAAGCAGCCAGTTACTATGAAGCGGAAGTTGATAACGCCGTAGACA
ATATGACGTCTTTATTAGAGCCAGCCATTATGGTGTTCTTAGGTGTTGTTATCGGCGGCATGATTGTCGCTATGTACTTG
CCGATTTTTGCCATGGGCGATGCTATTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (280-401)

• Type II secretion system (T2SS), protein F (T2SSF) (75-198)

3D structure

• AlphaFold DB: M5DNV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	58.477	99.511	0.582
pilC	Acinetobacter baumannii D1279779	55.611	98.044	0.545
pilC	Acinetobacter baylyi ADP1	54.115	98.044	0.531
pilC	Legionella pneumophila strain ERS1305867	51.372	98.044	0.504
pilG	Neisseria gonorrhoeae MS11	43.951	99.022	0.435
pilG	Neisseria meningitidis 44/76-A	43.704	99.022	0.433
pilC	Vibrio cholerae strain A1552	41.542	98.289	0.408
pilC	Vibrio campbellii strain DS40M4	39.698	97.311	0.386