Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TOL_RS03355 Genome accession   NC_020888
Coordinates   729884..731581 (-) Length   565 a.a.
NCBI ID   WP_015485866.1    Uniprot ID   M5E062
Organism   Thalassolituus oleivorans MIL-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 724884..736581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL_RS03330 (TOL_0686) - 725611..726792 (+) 1182 WP_015485861.1 response regulator -
  TOL_RS03335 (TOL_0687) yacG 726848..727042 (-) 195 WP_015485862.1 DNA gyrase inhibitor YacG -
  TOL_RS03340 (TOL_0688) coaE 727079..727678 (-) 600 WP_015485863.1 dephospho-CoA kinase -
  TOL_RS03345 (TOL_0689) - 727718..728605 (-) 888 WP_015485864.1 prepilin peptidase -
  TOL_RS03350 (TOL_0690) pilC 728610..729839 (-) 1230 WP_015485865.1 type II secretion system F family protein Machinery gene
  TOL_RS03355 (TOL_0691) pilB 729884..731581 (-) 1698 WP_015485866.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TOL_RS19385 - 731819..732313 (-) 495 WP_015485867.1 pilin -
  TOL_RS03365 (TOL_0693) nadC 732593..733444 (-) 852 WP_015485868.1 carboxylating nicotinate-nucleotide diphosphorylase -
  TOL_RS03370 (TOL_0694) - 733619..734962 (-) 1344 WP_015485869.1 alanine/glycine:cation symporter family protein -
  TOL_RS03375 (TOL_0695) ampD 735100..735639 (+) 540 WP_015485870.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  TOL_RS03380 (TOL_0696) ampE 735700..736575 (+) 876 WP_015485871.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61712.81 Da        Isoelectric Point: 5.0220

>NTDB_id=57630 TOL_RS03355 WP_015485866.1 729884..731581(-) (pilB) [Thalassolituus oleivorans MIL-1]
MALLTGLPRRLVEQQLLNQAQAEEILASCKKEKQSFVTLAVAKKLVKAKELAVLASEEFGLPLLDLAAFDGELMPKELVS
SELIDKHRVLPLFVRGNRLFVAQSDPSQMIAIDEIKFNTGMTVEAILVEEDKLSVAVDRFLSANDNTLGDLDSALDDIDL
DDPNARDKEDEQNSNAGGDDAPIVRFVNKILLDAIKSGASDVHFEPYEKRYRVRYRTDGILLEVSSPPVNLAGKIAARLK
VMSQMDISERRLPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEPEQKDLYLSALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDLE
TAQIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCVHCKVPAEIPEHTLKEVGFTDE
LLADAEIFKPVGCEHCNHGYKGRVGIYEVVRVTDSISRIIMEGGNSIEISDAARKEGFADLRTSALKKVAQGVTSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=57630 TOL_RS03355 WP_015485866.1 729884..731581(-) (pilB) [Thalassolituus oleivorans MIL-1]
ATGGCGTTATTGACCGGCCTCCCTCGTCGTCTCGTTGAGCAACAACTTCTCAACCAAGCGCAAGCCGAAGAAATACTCGC
CAGTTGTAAAAAAGAAAAACAAAGTTTTGTCACACTGGCCGTTGCCAAAAAGTTAGTAAAAGCGAAAGAGCTTGCGGTGT
TAGCGTCCGAAGAATTCGGCTTACCGCTATTAGACCTAGCAGCATTTGACGGCGAATTGATGCCAAAAGAACTGGTCAGC
TCGGAACTCATCGACAAGCACCGTGTGCTACCACTATTTGTTCGCGGTAACCGATTATTTGTAGCGCAAAGCGATCCATC
GCAAATGATCGCTATCGACGAAATCAAGTTCAATACTGGCATGACGGTAGAAGCGATACTCGTTGAAGAAGATAAACTCA
GCGTTGCCGTTGATCGTTTTCTATCCGCCAACGACAATACCTTGGGCGATCTCGATAGCGCGCTCGACGACATTGATTTA
GATGACCCGAACGCCCGAGACAAAGAGGATGAACAAAATAGCAACGCCGGAGGCGATGACGCTCCTATCGTTCGCTTTGT
AAATAAAATTTTATTAGACGCAATTAAATCCGGAGCATCCGATGTTCACTTTGAGCCCTACGAAAAACGCTACCGTGTAC
GGTATCGTACCGATGGTATTCTATTAGAGGTCTCCAGCCCTCCAGTGAATTTGGCTGGTAAAATTGCGGCACGCCTAAAA
GTTATGTCGCAAATGGATATTTCTGAGCGTCGTCTTCCTCAAGATGGTCGTATCAAGATGAAGCTTTCAAAAACGAAAGC
CATCGACTTCCGCGTCAATACTTTACCCACGTTATGGGGCGAAAAAATCGTACTACGTATCCTTGATCCGTCTTCAGCTA
AACTAGGGATTGATGCTTTAGGCTATGAGCCAGAGCAAAAAGATTTGTACTTAAGTGCCTTGGCGAAACCACAAGGTATG
ATCTTAGTGACCGGACCTACCGGTTCTGGCAAGACGGTTTCGCTCTATACAGGCCTGAATATTTTAAACGTGGAAGGCAC
CAATATTTCCACGGCAGAAGATCCCGTTGAAATTAACTTGGAAGGTATCAACCAAGTTAACGTGAATGCTCGTGTCGGTC
TCGACTTTGCTGCCGCGCTGCGATCCTTCTTACGCCAAGATCCTGATGTGGTCATGGTGGGTGAGATTCGCGATTTGGAA
ACTGCTCAAATTGCGATTAAAGCCGCACAAACAGGTCACTTAGTGCTGTCTACTCTACACACCAATAGTGCAGCCGAAAC
ACTGACACGTTTGGCCAATATGGGGGTTCCGTCGTTTAATATTGCCACTTCAGTCAGTTTAATTATTGCTCAGCGCTTAG
CTCGCCGCCTATGCGTGCACTGTAAAGTCCCCGCAGAAATCCCAGAGCACACACTGAAAGAAGTGGGCTTTACCGATGAA
TTACTTGCAGACGCCGAAATATTTAAACCAGTGGGTTGTGAGCACTGCAACCATGGCTACAAAGGCCGCGTCGGTATATA
TGAAGTCGTTCGCGTTACCGATTCGATCTCCCGCATCATCATGGAAGGTGGTAACTCCATTGAAATTTCGGATGCTGCTC
GAAAAGAAGGATTTGCCGATTTGCGTACTTCGGCGCTTAAGAAAGTCGCTCAAGGCGTCACTAGCCTAGAAGAAGCAAAC
CGTGTGACAACCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5E062

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.655

100

0.627
  pilB Acinetobacter baylyi ADP1

62.081

100

0.623
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.552
  pilB Vibrio parahaemolyticus RIMD 2210633

51.779

99.469

0.515
  pilB Vibrio cholerae strain A1552

50.794

100

0.51
  pilB Vibrio campbellii strain DS40M4

50.353

100

0.504
  pilF Neisseria gonorrhoeae MS11

50.177

99.823

0.501
  pilF Thermus thermophilus HB27

36.46

100

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

40.837

88.85

0.363

Multiple sequence alignment