Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KMZ15_RS01005 Genome accession   NZ_CP076444
Coordinates   275337..276608 (-) Length   423 a.a.
NCBI ID   WP_223694621.1    Uniprot ID   -
Organism   Mycoavidus sp. HKI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 270337..281608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ15_RS00975 (KMZ15_00965) - 270606..271043 (+) 438 WP_223694619.1 NUDIX domain-containing protein -
  KMZ15_RS00980 (KMZ15_00970) - 271079..271267 (-) 189 WP_223693234.1 DNA gyrase inhibitor YacG -
  KMZ15_RS00985 (KMZ15_00975) zapD 271366..272124 (-) 759 WP_223693236.1 cell division protein ZapD -
  KMZ15_RS00990 (KMZ15_00980) coaE 272303..272926 (-) 624 WP_223693238.1 dephospho-CoA kinase -
  KMZ15_RS00995 (KMZ15_00985) - 273053..274048 (-) 996 WP_223693241.1 A24 family peptidase -
  KMZ15_RS01000 (KMZ15_00990) - 274102..275328 (-) 1227 WP_223693243.1 type II secretion system F family protein -
  KMZ15_RS01005 (KMZ15_00995) pilF 275337..276608 (-) 1272 WP_223694621.1 GspE/PulE family protein Machinery gene
  KMZ15_RS01010 (KMZ15_01000) - 276836..278131 (-) 1296 WP_223693245.1 HlyC/CorC family transporter -
  KMZ15_RS01015 (KMZ15_01005) - 278185..279114 (-) 930 WP_223693247.1 polyprenyl synthetase family protein -
  KMZ15_RS01020 (KMZ15_01010) - 279266..280075 (-) 810 WP_223693249.1 cytochrome c oxidase assembly protein -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45803.80 Da        Isoelectric Point: 6.8111

>NTDB_id=575292 KMZ15_RS01005 WP_223694621.1 275337..276608(-) (pilF) [Mycoavidus sp. HKI]
MNTLADSATAVPTPNALSSTGPKEDDNAPAVRLLRELLLDAATHGASDVHIEPSEHDWRIRLRIDGVLHETMRPPPYLRD
PFICCVKVLARLDIAERRVPQDGRLKLYVAADKFEEFRVNTLPTLWGEKLVLRRLDTLPATLTLTDLGFDLKQQATLAAA
IHAPHGMVLVTGPTGSGKTLSLYCFLQRLNRESINICSVEDPAEIQLAGVNQVSVRDKAGLTFAVALRAFLRQDPDVIMV
GEIRDTETASVALQAAQTGHLVLSTLHTNDAPATLARLLDMGIAPFNLACAMRLIIAQRLVRKLCLACRAPAFTDQTTAE
AMLRSAGFDETTLAEDWQPYRAVGCAACHGIGYRGRIGIHQVMPVTAAIRGLIMARSSTDAIAQQALRDGVKSLREAGLA
QVRAGTTSLEEILAVTDASPATF

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=575292 KMZ15_RS01005 WP_223694621.1 275337..276608(-) (pilF) [Mycoavidus sp. HKI]
ATGAATACGCTTGCGGATTCAGCAACTGCAGTACCGACTCCGAACGCCTTATCCTCTACTGGACCCAAAGAGGACGATAA
TGCTCCAGCTGTGCGACTATTGCGTGAACTTTTACTTGATGCTGCCACCCATGGCGCATCTGACGTGCATATTGAGCCTA
GCGAGCATGATTGGCGCATTCGGCTGCGCATCGATGGGGTACTGCATGAAACCATGCGCCCGCCGCCCTATCTAAGAGAT
CCGTTCATCTGTTGCGTGAAAGTGCTCGCTCGCCTCGATATTGCCGAGCGGCGCGTACCTCAAGACGGACGCCTCAAACT
ATACGTGGCGGCAGACAAATTTGAAGAATTCCGAGTGAATACACTACCCACCTTGTGGGGCGAAAAACTCGTTTTGCGGC
GACTTGACACCCTCCCAGCCACGCTTACTCTGACTGACCTCGGTTTTGACCTCAAACAACAAGCCACTCTGGCCGCAGCT
ATTCACGCCCCGCACGGCATGGTATTGGTTACAGGCCCCACGGGGAGCGGCAAAACTTTATCGTTATATTGCTTTTTACA
GCGCTTAAATCGAGAATCAATCAATATCTGCTCGGTCGAAGACCCAGCTGAAATACAATTAGCAGGCGTTAATCAGGTCA
GTGTACGCGACAAAGCGGGCCTCACCTTTGCCGTCGCACTGCGCGCCTTTTTACGACAAGATCCTGATGTGATTATGGTT
GGAGAAATTCGTGACACTGAAACCGCCAGTGTTGCACTACAAGCCGCACAAACGGGTCACCTTGTCTTATCCACCCTGCA
TACCAATGATGCTCCCGCTACACTAGCGCGTTTACTTGATATGGGCATAGCGCCTTTTAACTTGGCTTGCGCCATGCGCT
TAATTATCGCGCAAAGACTGGTCAGAAAACTCTGCTTAGCCTGTCGCGCCCCAGCTTTCACCGATCAGACAACTGCTGAG
GCGATGCTGCGTAGCGCGGGCTTTGACGAAACAACACTAGCAGAAGATTGGCAACCCTATCGAGCCGTCGGCTGCGCTGC
GTGTCATGGCATTGGTTATCGCGGCCGCATTGGCATTCATCAAGTGATGCCGGTTACCGCTGCGATACGCGGGCTCATCA
TGGCACGAAGCAGCACCGATGCAATCGCTCAACAAGCGCTGCGTGACGGCGTAAAAAGCTTACGAGAGGCGGGCTTAGCG
CAAGTACGCGCCGGCACAACCAGCCTCGAAGAAATTCTAGCCGTCACAGATGCCAGTCCGGCTACTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.525

93.617

0.492
  pilB Acinetobacter baumannii D1279779

50.246

95.981

0.482
  pilB Acinetobacter baylyi ADP1

51.282

92.199

0.473
  pilB Vibrio cholerae strain A1552

48.522

95.981

0.466
  pilB Legionella pneumophila strain ERS1305867

49.873

92.908

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

47.704

92.671

0.442
  pilB Vibrio campbellii strain DS40M4

47.449

92.671

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

42.131

97.636

0.411
  pilF Thermus thermophilus HB27

43.544

93.381

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

92.671

0.397
  pilB Haemophilus influenzae Rd KW20

40.602

94.326

0.383
  pilB Haemophilus influenzae 86-028NP

40.1

94.326

0.378
  pilB Glaesserella parasuis strain SC1401

45.455

80.615

0.366