Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KFS84_RS07510 Genome accession   NZ_CP076254
Coordinates   1694265..1695395 (+) Length   376 a.a.
NCBI ID   WP_009599961.1    Uniprot ID   A0A0K2ZME5
Organism   Xanthomonas translucens pv. graminis strain LMG 726     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1689265..1700395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS84_RS07485 (KFS84_07465) - 1689324..1689779 (-) 456 WP_009599965.1 DUF4426 domain-containing protein -
  KFS84_RS07490 (KFS84_07470) proC 1689794..1690636 (-) 843 WP_009599964.1 pyrroline-5-carboxylate reductase -
  KFS84_RS07495 (KFS84_07475) - 1690766..1691812 (-) 1047 WP_009599963.1 hypothetical protein -
  KFS84_RS07500 (KFS84_07480) - 1692304..1692954 (-) 651 WP_039956286.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFS84_RS07505 (KFS84_07485) pilT 1693079..1694116 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  KFS84_RS07510 (KFS84_07490) pilU 1694265..1695395 (+) 1131 WP_009599961.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFS84_RS07515 (KFS84_07495) - 1695651..1696190 (-) 540 WP_009599959.1 DNA-3-methyladenine glycosylase I -
  KFS84_RS07520 (KFS84_07500) - 1696206..1698016 (-) 1811 Protein_1483 DUF4153 domain-containing protein -
  KFS84_RS07525 (KFS84_07505) - 1698124..1698690 (+) 567 WP_009599956.1 YqgE/AlgH family protein -
  KFS84_RS07530 (KFS84_07510) ruvX 1698683..1699180 (+) 498 WP_053113829.1 Holliday junction resolvase RuvX -
  KFS84_RS07535 (KFS84_07515) - 1699200..1700147 (+) 948 WP_009599900.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41958.30 Da        Isoelectric Point: 6.8811

>NTDB_id=574080 KFS84_RS07510 WP_009599961.1 1694265..1695395(+) (pilU) [Xanthomonas translucens pv. graminis strain LMG 726]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=574080 KFS84_RS07510 WP_009599961.1 1694265..1695395(+) (pilU) [Xanthomonas translucens pv. graminis strain LMG 726]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGTATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCACCTGGTGTTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACTTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367