Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI797_RS18295 Genome accession   NZ_CP075564
Coordinates   3960816..3962522 (+) Length   568 a.a.
NCBI ID   WP_226170492.1    Uniprot ID   -
Organism   Aeromonas media strain TR3_1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3955816..3967522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI797_RS18270 (KI797_18210) pdhR 3956960..3957724 (-) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KI797_RS18275 (KI797_18215) ampD 3958001..3958573 (-) 573 WP_182787467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KI797_RS18280 (KI797_18220) - 3958719..3959180 (+) 462 WP_371316622.1 TIGR02281 family clan AA aspartic protease -
  KI797_RS18285 (KI797_18225) nadC 3959184..3960047 (+) 864 WP_226170489.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KI797_RS18290 (KI797_18230) - 3960360..3960812 (+) 453 WP_371316620.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KI797_RS18295 (KI797_18235) pilB 3960816..3962522 (+) 1707 WP_226170492.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  KI797_RS18300 (KI797_18240) pilC 3962652..3963893 (+) 1242 WP_043133310.1 type II secretion system F family protein Machinery gene
  KI797_RS18305 (KI797_18245) pilD 3963974..3964846 (+) 873 WP_042649027.1 A24 family peptidase Machinery gene
  KI797_RS18310 (KI797_18250) coaE 3964865..3965479 (+) 615 WP_226170495.1 dephospho-CoA kinase -
  KI797_RS18315 (KI797_18255) zapD 3965517..3966239 (+) 723 WP_226170498.1 cell division protein ZapD -
  KI797_RS18320 (KI797_18260) yacG 3966249..3966443 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  KI797_RS18325 (KI797_18265) mutT 3966519..3966923 (-) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62681.14 Da        Isoelectric Point: 5.8224

>NTDB_id=569688 KI797_RS18295 WP_226170492.1 3960816..3962522(+) (pilB) [Aeromonas media strain TR3_1]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYEIKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=569688 KI797_RS18295 WP_226170492.1 3960816..3962522(+) (pilB) [Aeromonas media strain TR3_1]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTCG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACACCATGTGCTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGATTCAGCTTCGGCCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAGCTCGCGGCTCGACGACAGCGTCAACACTGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGATCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGGCGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTCGATCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAACCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACGGTGTCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCAGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCGGCCCAGACCGGACACCTGGTACTGTCAACTCTGCACACCAACTCTGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.837

99.296

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37