Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KIP84_RS16930 Genome accession   NZ_CP075323
Coordinates   3529472..3531184 (+) Length   570 a.a.
NCBI ID   WP_174724348.1    Uniprot ID   -
Organism   Acinetobacter sp. BHS4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3524472..3536184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIP84_RS16895 (KIP84_16900) nusA 3525360..3526844 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  KIP84_RS16900 (KIP84_16905) rimP 3526882..3527406 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  KIP84_RS16920 (KIP84_16925) secG 3528044..3528373 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  KIP84_RS16925 (KIP84_16930) tpiA 3528386..3529180 (-) 795 WP_174723111.1 triose-phosphate isomerase -
  KIP84_RS16930 (KIP84_16935) pilB 3529472..3531184 (+) 1713 WP_174724348.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KIP84_RS16935 (KIP84_16940) pilC 3531211..3532437 (+) 1227 WP_125738580.1 type II secretion system F family protein Machinery gene
  KIP84_RS16940 (KIP84_16945) pilD 3532437..3533297 (+) 861 WP_002114758.1 A24 family peptidase Machinery gene
  KIP84_RS16945 (KIP84_16950) coaE 3533299..3533895 (+) 597 WP_032070755.1 dephospho-CoA kinase -
  KIP84_RS16950 (KIP84_16955) - 3533892..3534806 (-) 915 WP_174724350.1 DMT family transporter -
  KIP84_RS16955 (KIP84_16960) rlmB 3534842..3535591 (-) 750 WP_213688957.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KIP84_RS16960 (KIP84_16965) - 3535694..3536020 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63492.84 Da        Isoelectric Point: 4.9690

>NTDB_id=568784 KIP84_RS16930 WP_174724348.1 3529472..3531184(+) (pilB) [Acinetobacter sp. BHS4]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTNPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=568784 KIP84_RS16930 WP_174724348.1 3529472..3531184(+) (pilB) [Acinetobacter sp. BHS4]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCCCCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGTGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTTAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCCGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
CCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCAATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTATGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.81

92.105

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

36.655

98.596

0.361