Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI614_RS03245 Genome accession   NZ_CP075187
Coordinates   682474..684189 (-) Length   571 a.a.
NCBI ID   WP_226407848.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain 98     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677474..689189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI614_RS03220 (KI614_03220) aspS 677628..679427 (-) 1800 WP_226407840.1 aspartate--tRNA ligase -
  KI614_RS03225 (KI614_03225) - 679445..680059 (-) 615 WP_203469305.1 DUF502 domain-containing protein -
  KI614_RS03230 (KI614_03230) - 680059..680313 (-) 255 WP_226407842.1 FmdB family zinc ribbon protein -
  KI614_RS03235 (KI614_03235) pilD 680384..681232 (-) 849 WP_226407844.1 prepilin peptidase Machinery gene
  KI614_RS03240 (KI614_03240) pilC 681232..682458 (-) 1227 WP_226407846.1 type II secretion system F family protein Machinery gene
  KI614_RS03245 (KI614_03245) pilB 682474..684189 (-) 1716 WP_226407848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI614_RS03250 (KI614_03250) - 684268..685539 (-) 1272 WP_226407851.1 HlyC/CorC family transporter -
  KI614_RS03255 (KI614_03255) - 685543..686382 (-) 840 WP_226407853.1 inner membrane protein YpjD -
  KI614_RS03260 (KI614_03260) ffh 686412..687770 (+) 1359 WP_203468691.1 signal recognition particle protein -
  KI614_RS03265 (KI614_03265) - 687771..688382 (-) 612 WP_226407856.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62086.65 Da        Isoelectric Point: 5.7925

>NTDB_id=568481 KI614_RS03245 WP_226407848.1 682474..684189(-) (pilB) [Dechloromonas denitrificans strain 98]
MAATPQTQPLSGLARALVQAGRLKEAEAEQLLAQANTEKHSLIEQIIASQKASAIEIARFVADTFGYPLLDLAAFDEAHI
PETAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVTKYSESASETLKNLASD
DINLDFLDEDTSAKADEAAGQEIDDAPVVKFIQKMLLDAINEGASDIHFEPYEKYYRIRFRVDGILREIATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMRLVLSKNRAIDFRVSTLPTLQGEKIVLRILDPTSATLGIEALGYEPEQKAALLDAVN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNISTAEDPAEINLAGINQVNVDDKAGLTFPVALKAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSILLITAQRLARRLCTCKKPMTVPEQAMLDAG
FKPEELDGSWTLYGPGGCDRCKGSGYKGRVGIYQVMPVSEAMQRLIMSGATALDLALLAKNEGVKDLRQSGLLKVKQGVT
SLDEVLTTTNL

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=568481 KI614_RS03245 WP_226407848.1 682474..684189(-) (pilB) [Dechloromonas denitrificans strain 98]
ATGGCTGCAACTCCTCAAACACAACCCCTCAGTGGTCTGGCCCGCGCGCTGGTACAAGCCGGACGACTCAAGGAGGCGGA
GGCAGAGCAACTGCTGGCCCAGGCGAATACTGAAAAACATTCGCTGATCGAACAAATCATTGCCAGCCAGAAGGCATCCG
CCATTGAGATCGCACGATTTGTCGCTGACACATTCGGCTACCCGCTACTTGATCTTGCCGCCTTCGACGAAGCACACATT
CCGGAAACAGCAATTGATCGCAAATTAATTGCGACCCACAAAGTCATTCCGCTCAACAAACGCGGCAATCGTCTTTCTGT
GGCCATTGCCGACCCGACCAATTTACGTGCGCTGGACGAAATCCGCTTCCAGACCGGCCTGGCCGTCGACCCCATTGTTG
TCGAACATCCCAAACTTGCACCGCTTGTCACGAAATACTCGGAGTCTGCGTCAGAAACCTTGAAAAATCTGGCCAGCGAC
GACATCAACCTCGATTTCCTCGACGAAGACACCAGCGCAAAAGCCGATGAGGCTGCAGGGCAAGAAATCGACGACGCCCC
CGTTGTCAAATTCATCCAGAAAATGCTGCTTGATGCGATCAATGAGGGGGCGTCGGATATTCATTTCGAGCCCTATGAAA
AGTACTACCGGATTCGTTTCCGGGTCGACGGCATACTCCGCGAAATCGCCACCCCCCCGTTGGCCATCAAGGAAAAGATC
GCTTCACGCATCAAGGTCATTTCGCGTTTGAACATTGCAGAAAAGCGCGTCCCGCAAGACGGTCGCATGCGCTTGGTCCT
GTCAAAAAATCGGGCCATCGACTTTCGGGTCAGCACGCTGCCCACCCTTCAGGGCGAGAAAATCGTCTTGCGTATTCTCG
ACCCAACGTCTGCCACGCTTGGTATTGAAGCACTGGGTTACGAGCCGGAGCAGAAAGCCGCTCTGCTTGATGCGGTCAAC
CGGCCATACGGCATGGTTTTGGTCACAGGTCCTACCGGCTCCGGCAAGACAGTCTCGCTATACACCTGCCTAAACATCCT
CAACAAGGATGGCGTCAACATTTCCACGGCCGAGGACCCAGCTGAAATCAATTTGGCGGGTATCAATCAGGTCAATGTGG
ACGACAAAGCCGGACTCACCTTTCCTGTCGCCCTGAAAGCCTTTCTCCGCCAGGATCCGGACGTCATCATGGTGGGTGAA
ATTCGGGATCTCGAGACCGCTGAAATCGCCATCAAGGCCGCTCAGACAGGGCATATGGTGCTCTCGACGCTACACACCAA
CGATGCACCACAAACACTGACGCGACTGATGAATATGGGTGTTCCCACATTCAACATCGCGTCGAGCATCTTGTTGATTA
CCGCACAGCGCTTGGCACGCAGGCTTTGCACCTGCAAGAAACCAATGACCGTCCCCGAGCAGGCAATGCTCGATGCAGGC
TTTAAGCCAGAAGAACTGGATGGTTCATGGACGCTTTATGGGCCGGGTGGCTGTGATCGCTGCAAAGGCAGCGGCTATAA
AGGCCGGGTCGGCATTTATCAGGTCATGCCGGTCTCGGAGGCCATGCAGCGCTTGATCATGAGCGGTGCCACGGCGCTGG
ATCTGGCCCTCCTGGCTAAAAATGAGGGGGTCAAGGATTTGCGTCAGTCCGGACTGCTCAAGGTCAAGCAAGGAGTTACG
TCGCTTGATGAAGTATTGACCACGACCAATTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.72

100

0.548
  pilB Acinetobacter baumannii D1279779

53.497

100

0.536
  pilB Legionella pneumophila strain ERS1305867

52.696

100

0.531
  pilF Neisseria gonorrhoeae MS11

51.318

99.65

0.511
  pilB Vibrio cholerae strain A1552

48.763

99.124

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

48.41

99.124

0.48
  pilB Vibrio campbellii strain DS40M4

48.663

98.249

0.478
  pilF Thermus thermophilus HB27

38.169

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.475

98.774

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

41.118

87.741

0.361