Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LILO_RS05835 Genome accession   NC_020450
Coordinates   1204290..1205138 (+) Length   282 a.a.
NCBI ID   WP_015426410.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis IO-1     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1189549..1255793 1204290..1205138 within 0


Gene organization within MGE regions


Location: 1189549..1255793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LILO_RS05775 (lilo_1107) - 1190925..1192466 (+) 1542 WP_015426398.1 2-isopropylmalate synthase -
  LILO_RS05780 (lilo_1108) leuB 1192478..1193515 (+) 1038 WP_042230337.1 3-isopropylmalate dehydrogenase -
  LILO_RS05785 (lilo_1109) leuC 1193712..1195094 (+) 1383 WP_015426400.1 3-isopropylmalate dehydratase large subunit -
  LILO_RS05790 (lilo_1110) leuD 1195114..1195689 (+) 576 WP_015426401.1 3-isopropylmalate dehydratase small subunit -
  LILO_RS05795 (lilo_1111) - 1195703..1196482 (+) 780 WP_015426402.1 ABC transporter ATP-binding protein -
  LILO_RS05800 (lilo_1112) ilvD 1196604..1198316 (+) 1713 WP_015426403.1 dihydroxy-acid dehydratase -
  LILO_RS05805 (lilo_1113) - 1198327..1200054 (+) 1728 WP_015426404.1 acetolactate synthase large subunit -
  LILO_RS05810 (lilo_1114) ilvN 1200047..1200523 (+) 477 WP_015426405.1 acetolactate synthase small subunit -
  LILO_RS05815 (lilo_1115) ilvC 1200569..1201591 (+) 1023 WP_015426406.1 ketol-acid reductoisomerase -
  LILO_RS05820 (lilo_1116) ilvA 1201647..1202897 (+) 1251 WP_015426407.1 threonine ammonia-lyase IlvA -
  LILO_RS05825 (lilo_1117) budA 1203013..1203723 (+) 711 WP_015426408.1 acetolactate decarboxylase -
  LILO_RS05830 (lilo_1118) - 1203764..1204144 (+) 381 WP_015426409.1 RidA family protein -
  LILO_RS05835 (lilo_1119) dprA 1204290..1205138 (+) 849 WP_015426410.1 DNA-processing protein DprA Machinery gene
  LILO_RS05840 (lilo_1120) topA 1205400..1207532 (+) 2133 WP_015426411.1 type I DNA topoisomerase -
  LILO_RS05845 (lilo_1121) trmFO 1207604..1208947 (+) 1344 WP_003131150.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  LILO_RS05850 (lilo_1122) xerS 1208992..1210062 (-) 1071 WP_015426412.1 tyrosine recombinase XerS -
  LILO_RS05855 (lilo_1123) - 1210547..1210834 (-) 288 WP_015426413.1 hypothetical protein -
  LILO_RS05860 (lilo_1124) xerS 1210918..1211973 (-) 1056 WP_015426414.1 tyrosine recombinase XerS -
  LILO_RS11960 - 1212514..1212801 (+) 288 WP_148284411.1 hypothetical protein -
  LILO_RS05865 (lilo_1125) - 1212918..1213412 (+) 495 WP_015426415.1 hypothetical protein -
  LILO_RS05870 (lilo_1126) recA 1213604..1214629 (-) 1026 WP_042230342.1 recombinase RecA -
  LILO_RS05875 - 1214644..1215036 (-) 393 WP_042230345.1 hypothetical protein -
  LILO_RS05880 (lilo_1127) - 1215033..1216484 (-) 1452 WP_015426417.1 Y-family DNA polymerase -
  LILO_RS05885 (lilo_1128) - 1216680..1217477 (+) 798 WP_015426418.1 hypothetical protein -
  LILO_RS05890 (lilo_1129) - 1217692..1218144 (-) 453 WP_015426419.1 ASCH domain-containing protein -
  LILO_RS05905 (lilo_1132) - 1220040..1220897 (-) 858 WP_015426422.1 hypothetical protein -
  LILO_RS05910 - 1220894..1221376 (-) 483 WP_052312053.1 Mov34/MPN/PAD-1 family protein -
  LILO_RS05915 (lilo_1134) - 1221366..1223042 (-) 1677 WP_042230350.1 ThiF family adenylyltransferase -
  LILO_RS05920 (lilo_1136) - 1223055..1224077 (-) 1023 WP_015426426.1 cyclic GMP-AMP synthase DncV-like nucleotidyltransferase -
  LILO_RS05925 - 1224245..1224445 (-) 201 WP_042230352.1 hypothetical protein -
  LILO_RS11740 (lilo_1138) - 1224823..1225971 (+) 1149 WP_148284413.1 restriction endonuclease subunit S -
  LILO_RS05935 (lilo_1139) - 1226058..1226987 (+) 930 WP_042230355.1 site-specific integrase -
  LILO_RS05940 (lilo_1140) - 1227021..1228271 (-) 1251 WP_042230357.1 restriction endonuclease subunit S -
  LILO_RS05945 (lilo_1141) - 1228273..1229856 (-) 1584 WP_015426431.1 type I restriction-modification system subunit M -
  LILO_RS05950 (lilo_1142) - 1229856..1232927 (-) 3072 WP_015426432.1 type I restriction endonuclease subunit R -
  LILO_RS12410 - 1233243..1233326 (-) 84 WP_414843018.1 putative holin-like toxin -
  LILO_RS05955 (lilo_1143) - 1233472..1235604 (-) 2133 WP_052312054.1 Eco57I restriction-modification methylase domain-containing protein -
  LILO_RS05960 (lilo_1144) - 1235733..1237007 (-) 1275 WP_042230729.1 hypothetical protein -
  LILO_RS12415 - 1237875..1237946 (-) 72 Protein_1160 IS6 family transposase -
  LILO_RS05965 (lilo_1145) - 1238052..1239572 (-) 1521 WP_015426435.1 relaxase/mobilization nuclease domain-containing protein -
  LILO_RS05970 - 1239569..1239955 (-) 387 WP_042230360.1 plasmid mobilization protein -
  LILO_RS05975 (lilo_1147) - 1240248..1241294 (-) 1047 WP_015426437.1 hypothetical protein -
  LILO_RS11745 (lilo_1148) - 1241320..1243287 (-) 1968 WP_052312055.1 ArdC-like ssDNA-binding domain-containing protein -
  LILO_RS05985 - 1243442..1244557 (-) 1116 WP_042230362.1 toprim domain-containing protein -
  LILO_RS05990 (lilo_1149) - 1244560..1245753 (-) 1194 WP_015426439.1 hypothetical protein -
  LILO_RS05995 (lilo_1150) - 1245866..1247239 (-) 1374 WP_015426440.1 hypothetical protein -
  LILO_RS06000 - 1247242..1247517 (-) 276 WP_042230365.1 hypothetical protein -
  LILO_RS06005 (lilo_1151) - 1247510..1248112 (-) 603 WP_015426441.1 hypothetical protein -
  LILO_RS06010 (lilo_1152) - 1248125..1250734 (-) 2610 WP_015426442.1 phage tail tip lysozyme -
  LILO_RS06015 (lilo_1153) - 1250721..1253138 (-) 2418 WP_015426443.1 VirB4-like conjugal transfer ATPase, CD1110 family -
  LILO_RS06020 - 1253116..1253454 (-) 339 WP_042230368.1 PrgI family protein -
  LILO_RS06025 (lilo_1155) - 1253457..1254308 (-) 852 WP_015426445.1 hypothetical protein -
  LILO_RS06030 - 1254455..1254829 (-) 375 WP_042230370.1 hypothetical protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32159.28 Da        Isoelectric Point: 9.5086

>NTDB_id=56468 LILO_RS05835 WP_015426410.1 1204290..1205138(+) (dprA) [Lactococcus lactis subsp. lactis IO-1]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDIIKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=56468 LILO_RS05835 WP_015426410.1 1204290..1205138(+) (dprA) [Lactococcus lactis subsp. lactis IO-1]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACATAATTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCTGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTAATTCTATCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGCAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATTCAACAAGGAGCAAA
ATTAATCTACCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.525

100

0.865
  dprA Streptococcus mutans UA159

60.072

98.582

0.592
  dprA/cilB/dalA Streptococcus mitis SK321

54.643

99.291

0.543
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.929

99.291

0.535
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

38.519

95.745

0.369
  dprA Bacillus subtilis subsp. subtilis str. 168

44.206

82.624

0.365
  dprA Latilactobacillus sakei subsp. sakei 23K

40

90.426

0.362

Multiple sequence alignment