Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KEC58_RS01925 Genome accession   NZ_CP073684
Coordinates   385970..387661 (-) Length   563 a.a.
NCBI ID   WP_284632385.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd1605     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380970..392661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEC58_RS01895 (KEC58_01895) mutT 381356..381778 (-) 423 WP_068968360.1 8-oxo-dGTP diphosphatase MutT -
  KEC58_RS01900 (KEC58_01900) yacG 381990..382199 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  KEC58_RS01905 (KEC58_01905) zapD 382208..382945 (-) 738 WP_005297594.1 cell division protein ZapD -
  KEC58_RS01910 (KEC58_01910) coaE 383013..383615 (-) 603 WP_151182736.1 dephospho-CoA kinase -
  KEC58_RS01915 (KEC58_01915) pilD 383617..384495 (-) 879 WP_106338474.1 A24 family peptidase Machinery gene
  KEC58_RS01920 (KEC58_01920) pilC 384678..385904 (-) 1227 WP_238087697.1 type II secretion system F family protein Machinery gene
  KEC58_RS01925 (KEC58_01925) pilB 385970..387661 (-) 1692 WP_284632385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KEC58_RS01930 (KEC58_01930) pilA 387668..388120 (-) 453 WP_238087699.1 pilin Machinery gene
  KEC58_RS01935 (KEC58_01935) ampD 388507..389085 (+) 579 WP_069531889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KEC58_RS01940 (KEC58_01940) pdhR 389445..390218 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62561.20 Da        Isoelectric Point: 5.3547

>NTDB_id=559948 KEC58_RS01925 WP_284632385.1 385970..387661(-) (pilB) [Photobacterium damselae strain Pdd1605]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDHTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETCYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNRGYLGRIGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=559948 KEC58_RS01925 WP_284632385.1 385970..387661(-) (pilB) [Photobacterium damselae strain Pdd1605]
ATGCACACCACTCTCGCCTCGATTCTGCACCAAGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGCATCTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATTTTTGGTCTCACCCTCGTTTCGGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGCCATCGCGCACTGCCCCTTGCTCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAACAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGTCGTAATATTGAACCGCTACTGCTAGAAAACAAACAGCTCGAATCAG
CTATCCGTCGTATTTATGGTCGCGAGTTGGGCGAAACCACTAATATGGCCAGTCGGGACGTCAGCGATGATGATCTAAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCACACCACAGATCTAGGCCAAGAAAACGAACCTGTTACCCGCTATAT
TAATCAAGTGCTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCTTATGAGACCTGTTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTACGAGTGTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATGACAGGGCCTACGGGTAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCTGAAGATCCGGTAGAGATCAACTTACCTGGTATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCCGTTAAAGCTGCGCAAACTGGGCACTTAGTGCTATCGACCTTGCATACCAATTCTGCCGCCGAAAC
CATTACCCGTTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACTCTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATTGACGGCTTTATGCAAGACAAACTGCGTATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAGAGGCTATTTAGGTCGCATCGGTATCTATGAAGT
GATGCCATTTAGTCGCCGTCTGGCTCAAGCTCTGGTTGATGGCGCGACTACCTTGGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.812

100

0.618
  pilB Vibrio parahaemolyticus RIMD 2210633

61.101

100

0.611
  pilB Vibrio campbellii strain DS40M4

60.568

100

0.606
  pilB Legionella pneumophila strain ERS1305867

49.816

96.625

0.481
  pilB Acinetobacter baylyi ADP1

49.088

97.336

0.478
  pilB Acinetobacter baumannii D1279779

49.624

94.494

0.469
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.499

92.185

0.364