Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   KEF49_RS08140 Genome accession   NZ_CP073635
Coordinates   1621899..1623149 (+) Length   416 a.a.
NCBI ID   WP_115997260.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   unknown (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1607334..1647323 1621899..1623149 within 0


Gene organization within MGE regions


Location: 1607334..1647323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS08080 (KEF49_08080) - 1607334..1609694 (+) 2361 WP_065981476.1 DNA translocase FtsK -
  KEF49_RS08085 (KEF49_08085) - 1609819..1610544 (+) 726 WP_212101398.1 GntR family transcriptional regulator -
  KEF49_RS08090 (KEF49_08090) - 1610656..1611885 (+) 1230 WP_065981478.1 MFS transporter -
  KEF49_RS08095 (KEF49_08095) - 1611989..1612444 (+) 456 WP_115997256.1 MarR family transcriptional regulator -
  KEF49_RS08100 (KEF49_08100) - 1612444..1615563 (+) 3120 WP_212101400.1 MMPL family transporter -
  KEF49_RS08105 (KEF49_08105) - 1615716..1616996 (+) 1281 WP_065981481.1 pitrilysin family protein -
  KEF49_RS08110 (KEF49_08110) - 1616993..1618279 (+) 1287 WP_065981482.1 pitrilysin family protein -
  KEF49_RS08115 (KEF49_08115) - 1618338..1619066 (+) 729 WP_212101670.1 SDR family oxidoreductase -
  KEF49_RS08120 (KEF49_08120) - 1619142..1619399 (+) 258 WP_003154150.1 DUF3243 domain-containing protein -
  KEF49_RS08125 (KEF49_08125) - 1619528..1620319 (+) 792 WP_065521268.1 YmfK family protein -
  KEF49_RS08130 (KEF49_08130) - 1620338..1621225 (+) 888 WP_065981483.1 RodZ domain-containing protein -
  KEF49_RS08135 (KEF49_08135) pgsA 1621300..1621881 (+) 582 WP_016936065.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  KEF49_RS08140 (KEF49_08140) cinA 1621899..1623149 (+) 1251 WP_115997260.1 competence/damage-inducible protein A Machinery gene
  KEF49_RS08145 (KEF49_08145) recA 1623321..1624364 (+) 1044 WP_013352318.1 recombinase RecA Machinery gene
  KEF49_RS08150 (KEF49_08150) - 1624530..1625711 (+) 1182 WP_212101403.1 serine hydrolase domain-containing protein -
  KEF49_RS08155 (KEF49_08155) rny 1626002..1627561 (+) 1560 WP_003154140.1 ribonuclease Y -
  KEF49_RS08160 (KEF49_08160) ymdB 1627623..1628417 (+) 795 WP_013352320.1 2',3'-cyclic-nucleotide 2'-phosphodiesterase -
  KEF49_RS08165 (KEF49_08165) spoVS 1628617..1628877 (+) 261 WP_003154135.1 stage V sporulation protein SpoVS -
  KEF49_RS08170 (KEF49_08170) tdh 1629134..1630180 (+) 1047 WP_115997261.1 L-threonine 3-dehydrogenase -
  KEF49_RS08175 (KEF49_08175) - 1630195..1631370 (+) 1176 WP_065981487.1 glycine C-acetyltransferase -
  KEF49_RS08180 (KEF49_08180) miaB 1631513..1633042 (+) 1530 WP_065981488.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  KEF49_RS08185 (KEF49_08185) - 1633045..1633476 (+) 432 WP_065981489.1 RicAFT regulatory complex protein RicA family protein -
  KEF49_RS08190 (KEF49_08190) cotE 1633729..1634274 (+) 546 WP_065981490.1 outer spore coat protein CotE -
  KEF49_RS08195 (KEF49_08195) mutS 1634390..1636975 (+) 2586 WP_115997807.1 DNA mismatch repair protein MutS -
  KEF49_RS08200 (KEF49_08200) mutL 1636991..1638868 (+) 1878 WP_115997262.1 DNA mismatch repair endonuclease MutL -
  KEF49_RS08205 (KEF49_08205) - 1638920..1640035 (-) 1116 WP_212101404.1 site-specific integrase -
  KEF49_RS08210 (KEF49_08210) - 1640151..1640976 (+) 826 Protein_1578 DNA adenine methylase -
  KEF49_RS08215 (KEF49_08215) - 1641092..1641610 (-) 519 WP_065981493.1 hypothetical protein -
  KEF49_RS08220 (KEF49_08220) - 1641748..1642182 (+) 435 WP_065981494.1 hypothetical protein -
  KEF49_RS08225 (KEF49_08225) - 1642327..1642704 (+) 378 WP_065981495.1 hypothetical protein -
  KEF49_RS08230 (KEF49_08230) - 1642736..1643323 (+) 588 WP_044051884.1 VanZ family protein -
  KEF49_RS08235 (KEF49_08235) - 1643635..1644021 (+) 387 WP_071346778.1 VOC family protein -
  KEF49_RS08240 (KEF49_08240) - 1644192..1644368 (+) 177 WP_193551599.1 hypothetical protein -
  KEF49_RS08245 (KEF49_08245) - 1644387..1644860 (+) 474 WP_065981497.1 ArpU family phage packaging/lysis transcriptional regulator -
  KEF49_RS08250 (KEF49_08250) - 1645322..1645606 (-) 285 WP_065981498.1 hypothetical protein -
  KEF49_RS19850 (KEF49_08255) - 1645612..1647323 (-) 1712 Protein_1587 T7SS effector LXG polymorphic toxin -

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 44975.22 Da        Isoelectric Point: 5.0599

>NTDB_id=559586 KEF49_RS08140 WP_115997260.1 1621899..1623149(+) (cinA) [Bacillus amyloliquefaciens strain Ba13]
MEIAKKAEIIAVGSELLLGQIANTNAQFISKELAEIGVNVFYHTAVGDNPERLKQVIRIAEERSDLIIFSGGLGPTKDDL
TKETIANTLGRPLVLNDEAFRSIEEYFAKTKRTMSPNNRKQALVIEGSDVLANHFGMAPGMLAEHGSRLYMLLPGPPSEL
RPMFENEAKPLLLKKLGTNEKIVSTVLRFFGIGESQLEADLEDIIDAQTNPTIAPLAADGEVTLRLTAKHADEKETERLL
KETEAAILERAGEFFYGYDDTSLVKELSKACRQNGITISSAESFTGGLFSEWVTDLSGASQLFAGGVVCYSDSVKQNVLG
VKAETLAESGAVSKECAKELAAGVRKLTGSDIGISFTGVAGPDPQEGHAPGRVFIGISAEGKEEVHEFNFAGSRTGIRKR
AAKYGCHLILKMLDQK

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=559586 KEF49_RS08140 WP_115997260.1 1621899..1623149(+) (cinA) [Bacillus amyloliquefaciens strain Ba13]
ATGGAAATAGCAAAAAAAGCGGAAATCATTGCAGTCGGCTCAGAGCTTCTGCTCGGCCAGATCGCCAATACGAATGCGCA
GTTTATCAGTAAAGAGCTGGCGGAAATCGGCGTAAATGTGTTTTATCATACCGCTGTCGGAGACAATCCGGAGCGGCTTA
AGCAAGTCATCCGCATTGCGGAAGAGCGCTCAGACCTGATTATTTTTTCTGGCGGCCTCGGACCGACCAAGGACGATTTA
ACAAAAGAAACCATTGCAAACACTTTGGGGCGCCCGCTCGTTCTGAATGACGAAGCGTTCCGGTCGATTGAAGAGTATTT
CGCGAAAACAAAACGGACAATGTCGCCGAATAACCGCAAACAGGCGCTCGTCATTGAAGGCTCAGACGTTTTGGCGAATC
ACTTCGGCATGGCGCCGGGAATGCTTGCAGAGCACGGCTCAAGACTTTATATGCTGCTCCCTGGGCCGCCGAGCGAGCTG
CGTCCGATGTTTGAAAATGAAGCGAAGCCGCTGCTTCTTAAAAAGCTCGGTACAAATGAGAAGATCGTTTCAACCGTGCT
GCGGTTTTTCGGAATCGGTGAATCGCAGCTTGAAGCTGATTTAGAAGATATCATTGATGCGCAGACGAACCCGACGATTG
CGCCGCTTGCTGCCGACGGCGAGGTCACGCTGCGTTTAACGGCGAAACATGCCGACGAAAAAGAAACAGAGCGGCTTTTA
AAAGAAACGGAAGCCGCCATTTTGGAACGTGCCGGTGAATTTTTCTACGGATATGATGATACGTCGCTGGTGAAGGAGCT
TTCAAAAGCATGCAGACAAAACGGAATCACGATTTCCTCTGCCGAAAGCTTTACCGGAGGCCTGTTTTCAGAGTGGGTGA
CTGATTTAAGCGGTGCGTCTCAACTATTTGCGGGGGGCGTTGTCTGCTATTCCGACAGCGTGAAACAGAATGTGCTCGGC
GTGAAAGCTGAAACGCTCGCAGAAAGCGGAGCGGTCAGCAAAGAGTGCGCGAAAGAGCTTGCCGCCGGTGTAAGAAAGCT
GACCGGAAGCGATATCGGCATCAGCTTTACCGGAGTTGCGGGCCCCGATCCGCAGGAAGGGCATGCCCCGGGCCGCGTCT
TTATCGGTATTTCCGCGGAAGGGAAAGAAGAGGTGCACGAATTTAACTTTGCCGGCTCAAGAACCGGAATCAGAAAGCGC
GCCGCTAAATACGGCTGCCATCTTATTTTGAAAATGCTGGATCAAAAATAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(261-413)

Damage-inducible protein CinA KH domain (CinA_KH)

(184-257)

Probable molybdopterin binding domain (MoCF_biosynth)

(9-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Bacillus subtilis subsp. subtilis str. 168

90.144

100

0.901
  cinA Streptococcus mitis NCTC 12261

48.086

100

0.483
  cinA Streptococcus mitis SK321

47.608

100

0.478
  cinA Streptococcus pneumoniae TIGR4

47.129

100

0.474
  cinA Streptococcus pneumoniae Rx1

47.129

100

0.474
  cinA Streptococcus pneumoniae R6

47.129

100

0.474
  cinA Streptococcus pneumoniae D39

46.89

100

0.471
  cinA Streptococcus mutans UA159

46.731

99.279

0.464
  cinA Streptococcus suis isolate S10

40.153

93.99

0.377