Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KCV10_RS05650 Genome accession   NZ_CP073243
Coordinates   1233991..1235703 (+) Length   570 a.a.
NCBI ID   WP_151682978.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain S63-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228991..1240703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCV10_RS05615 nusA 1229881..1231365 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  KCV10_RS05620 rimP 1231403..1231927 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  KCV10_RS05640 secG 1232565..1232894 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  KCV10_RS05645 tpiA 1232907..1233701 (-) 795 WP_049064232.1 triose-phosphate isomerase -
  KCV10_RS05650 pilB 1233991..1235703 (+) 1713 WP_151682978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCV10_RS05655 pilC 1235731..1236957 (+) 1227 WP_019456773.1 type II secretion system F family protein Machinery gene
  KCV10_RS18675 pilD 1236957..1237817 (+) 861 WP_151760695.1 prepilin peptidase Machinery gene
  KCV10_RS05665 coaE 1237819..1238415 (+) 597 WP_151682980.1 dephospho-CoA kinase -
  KCV10_RS05670 - 1238412..1239326 (-) 915 WP_151682981.1 DMT family transporter -
  KCV10_RS05675 rlmB 1239362..1240111 (-) 750 WP_151682982.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KCV10_RS05680 - 1240214..1240540 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63443.66 Da        Isoelectric Point: 5.0230

>NTDB_id=558437 KCV10_RS05650 WP_151682978.1 1233991..1235703(+) (pilB) [Acinetobacter seifertii strain S63-3]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEAHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPTDVPKQSLLEMGF
TEQDLAHPDFCVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLQKVIQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=558437 KCV10_RS05650 WP_151682978.1 1233991..1235703(+) (pilB) [Acinetobacter seifertii strain S63-3]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGCGCATCTCTCTC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAAACCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAGGATGAAGACGAAACACCTCAAGGTGATGAAGCACCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGAGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCTAATCCGCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCCCTAGGATACGAAGAAGAACAAAAAGCTTTGTTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCGA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTTGGTGAGATTCGT
GATTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGTCTTTGCTCTCAGTGTAAAATACCTACAGATGTTCCAAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGACTTAGCACATCCAGATTTTTGCGTTTTTCAACCGGTAGGTTGTTCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTAGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTCTAGAAA
TCGCGGCCGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTGCAAAAGGTAATACAAGGGGTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.491

100

0.965
  pilB Acinetobacter baylyi ADP1

79.298

100

0.793
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.224

93.158

0.477
  pilB Vibrio cholerae strain A1552

47.88

99.298

0.475
  pilB Vibrio campbellii strain DS40M4

50.669

91.754

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.655

98.596

0.361