Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KCV09_RS10185 Genome accession   NZ_CP073241
Coordinates   2212712..2214427 (+) Length   571 a.a.
NCBI ID   WP_119689887.1    Uniprot ID   -
Organism   Acinetobacter soli strain M3-1-68     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2207712..2219427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCV09_RS10150 nusA 2208191..2209675 (-) 1485 WP_004943981.1 transcription termination factor NusA -
  KCV09_RS10155 rimP 2209711..2210235 (-) 525 WP_004932478.1 ribosome maturation factor RimP -
  KCV09_RS10175 secG 2211312..2211641 (-) 330 WP_004932477.1 preprotein translocase subunit SecG -
  KCV09_RS10180 tpiA 2211651..2212448 (-) 798 WP_004932474.1 triose-phosphate isomerase -
  KCV09_RS10185 pilB 2212712..2214427 (+) 1716 WP_119689887.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCV09_RS10190 pilC 2214451..2215677 (+) 1227 WP_004943990.1 type II secretion system F family protein Machinery gene
  KCV09_RS10195 pilD 2215677..2216537 (+) 861 WP_151814992.1 A24 family peptidase Machinery gene
  KCV09_RS10200 coaE 2216540..2217139 (+) 600 WP_151814991.1 dephospho-CoA kinase -
  KCV09_RS10205 - 2217115..2218044 (-) 930 WP_025095298.1 DMT family transporter -
  KCV09_RS10210 rlmB 2218082..2218828 (-) 747 WP_004943999.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KCV09_RS10215 - 2218930..2219256 (-) 327 WP_004932453.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 64192.82 Da        Isoelectric Point: 5.1748

>NTDB_id=558397 KCV09_RS10185 WP_119689887.1 2212712..2214427(+) (pilB) [Acinetobacter soli strain M3-1-68]
MSLVTASPKFSGFIRRLVEEGHIDAHDMQMAQESAKKNKQDIVPYLIDHYAITPITIAETISVEFGEPLFDLDVFDPALI
LKDRIDEKLIRKYRVMPLVHRGHVLYVATCNPTNLEAMDAIRFNSKLKVEPIIVEHDKLERLLNEYFPEDSRFNFDTEDF
DLDLEVNSPTSDDDDDDEISKDEAPVVKYINKLLIDAIRMNASDLHFEPYEKMYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFTEALNRP
QGMLLITGPTGSGKTVSMYTGLNILNTEHANISTAEDPVEINLEGINQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRSMGVATFNIATSVNLVIAQRLARRLCNQCKRRIDVPRASLLEMGF
TETDLDAPDFQIYEPVGCKECREGYKGRVGIYEVMKITPEISRIIMQDGNALEIAAAAEQLGFSNLRRSGLKKVMQGVTS
LQEVNRVTNEE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=558397 KCV09_RS10185 WP_119689887.1 2212712..2214427(+) (pilB) [Acinetobacter soli strain M3-1-68]
ATGTCATTAGTTACCGCATCACCTAAATTTTCAGGATTCATTCGGCGCCTTGTCGAAGAAGGTCATATTGATGCACACGA
CATGCAAATGGCTCAAGAGAGCGCAAAAAAAAACAAGCAAGATATTGTTCCTTATTTGATCGACCATTATGCCATTACCC
CGATCACCATTGCTGAAACCATCTCGGTCGAGTTTGGCGAACCGCTATTTGATCTGGATGTTTTTGATCCTGCGCTGATT
CTCAAAGATCGTATCGATGAAAAGCTGATTCGCAAGTATCGTGTAATGCCGTTGGTTCATCGTGGGCATGTGCTCTATGT
CGCGACCTGCAATCCGACCAATCTTGAAGCGATGGATGCCATCCGTTTTAACAGCAAACTCAAAGTCGAACCGATTATTG
TAGAACACGATAAGCTTGAGCGACTGTTAAACGAATATTTCCCCGAAGATAGCCGTTTTAACTTTGACACCGAAGATTTT
GATCTGGATCTGGAGGTGAATTCCCCCACTTCAGATGACGACGACGATGACGAAATCAGTAAAGATGAAGCCCCGGTGGT
CAAATACATCAATAAATTACTGATTGATGCTATTCGTATGAATGCATCAGACTTACATTTTGAACCTTATGAAAAAATGT
ATCGGGTCCGTTATCGGGTCGATGGTGTACTTCGTTTAATTGCAACACCACCCTTACAACTGGCTACCCGACTGGCCTCG
CGTTTGAAAGTGATGTCTCAAATGGATATTTCGGAAAAGCGTGTTCCGCAAGATGGTCGTATTAAACTGAAACTGTCGAA
AACCAAAACCATCGATTTTCGGGTCAACTCCCTACCGACCCTATTTGGTGAAAAGATTGTACTGCGGATTCTGGATCCTG
CGAGCGCGATGCTGGGTATCGATGCACTGGGCTACGAGCCTGAGCAAAAAGCATTGTTTACCGAAGCGCTGAATCGACCA
CAAGGCATGCTGCTGATTACCGGCCCTACAGGCTCGGGGAAAACCGTATCGATGTATACCGGCTTAAATATTCTCAATAC
CGAACATGCCAATATTTCTACCGCCGAAGATCCAGTCGAGATTAATCTTGAAGGTATTAATCAGGTCAATGTAAATCCCA
AAGTCGGCCTGACCTTTGCTGCGGCATTACGCTCTTTTTTACGTCAAGACCCTGATATCATTATGGTGGGGGAAATTCGT
GATTTAGAAACCGCAGAGATTGCCATTAAAGCGGCTCAAACCGGTCACTTGGTGATGTCGACACTGCATACCAATAACGC
TGCCGAAACACTTACACGCCTGAGAAGCATGGGGGTGGCGACCTTTAATATCGCCACCTCGGTCAATCTGGTGATTGCGC
AGCGCCTTGCCAGACGCCTGTGTAATCAGTGTAAACGGCGTATCGACGTGCCACGTGCCAGTTTACTCGAAATGGGATTT
ACCGAAACCGATTTAGATGCTCCAGACTTTCAGATCTATGAACCGGTGGGGTGTAAAGAATGTCGGGAGGGCTACAAAGG
ACGTGTGGGCATTTATGAAGTCATGAAAATTACACCAGAAATCTCAAGAATTATTATGCAAGACGGCAATGCACTTGAAA
TTGCAGCAGCCGCTGAACAGCTTGGATTTAGCAACTTACGCCGTTCTGGCTTAAAAAAAGTGATGCAGGGGGTCACATCG
TTACAGGAAGTCAATCGTGTGACGAATGAAGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

86.667

99.825

0.865
  pilB Acinetobacter baumannii D1279779

77.544

99.825

0.774
  pilB Legionella pneumophila strain ERS1305867

51.304

100

0.517
  pilF Neisseria gonorrhoeae MS11

49.644

98.424

0.489
  pilB Vibrio cholerae strain A1552

51.644

90.543

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.577

91.068

0.461
  pilB Vibrio campbellii strain DS40M4

50.097

90.543

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

37.77

97.373

0.368