Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   M7W_RS11380 Genome accession   NC_020207
Coordinates   2339915..2340790 (-) Length   291 a.a.
NCBI ID   WP_010738639.1    Uniprot ID   -
Organism   Enterococcus faecium ATCC 8459 = NRRL B-2354     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2334915..2345790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M7W_RS11345 (M7W_2471) - 2335918..2336925 (-) 1008 WP_002286175.1 class I SAM-dependent methyltransferase -
  M7W_RS11350 (M7W_2472) comGG 2337097..2337456 (-) 360 WP_002293655.1 competence type IV pilus minor pilin ComGG -
  M7W_RS11355 (M7W_2473) comGF 2337428..2337853 (-) 426 WP_002325315.1 competence type IV pilus minor pilin ComGF -
  M7W_RS11360 (M7W_2474) - 2337816..2338163 (-) 348 WP_002286177.1 hypothetical protein -
  M7W_RS11365 (M7W_2475) comGD 2338135..2338605 (-) 471 WP_002317395.1 competence type IV pilus minor pilin ComGD -
  M7W_RS11370 (M7W_2476) comGC 2338580..2338858 (-) 279 WP_002286179.1 competence type IV pilus major pilin ComGC -
  M7W_RS11375 (M7W_2477) comGB 2338855..2339904 (-) 1050 WP_002286180.1 competence type IV pilus assembly protein ComGB -
  M7W_RS11380 (M7W_2478) comYA 2339915..2340790 (-) 876 WP_010738639.1 competence type IV pilus ATPase ComGA Machinery gene
  M7W_RS11385 (M7W_2479) ade 2340922..2342676 (+) 1755 WP_002286185.1 adenine deaminase -
  M7W_RS11390 (M7W_2480) trmB 2342774..2343436 (-) 663 WP_002286189.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  M7W_RS11395 (M7W_2481) - 2343506..2344288 (-) 783 WP_002286194.1 phosphotransferase family protein -
  M7W_RS11400 (M7W_2482) - 2344492..2345703 (-) 1212 WP_002286197.1 ABC transporter permease -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 33610.75 Da        Isoelectric Point: 9.0680

>NTDB_id=55777 M7W_RS11380 WP_010738639.1 2339915..2340790(-) (comYA) [Enterococcus faecium ATCC 8459 = NRRL B-2354]
MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFWKKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTY
SLEKQEVRLRLSSVGDYLQRESLVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAAVRAALTGHRVFATVHARNLEGT
LVRIHELTKKKEELSECLAGVIYQELLEDANHHPSVLWSYQFMEEESVKKS

Nucleotide


Download         Length: 876 bp        

>NTDB_id=55777 M7W_RS11380 WP_010738639.1 2339915..2340790(-) (comYA) [Enterococcus faecium ATCC 8459 = NRRL B-2354]
ATGGAAATAAAACGATTATCGGATCAAATGATAAGTGAAAGTTTTGCCAAACATGCCCAAGACGTATATATATTGCCAGC
TTCAGAAGTATTTTACATCTATTTTCGCTATGGAAAAAAACGAACATTCTGGAAGAAAGTCTCTTCGGCAGATGGACAGC
AACTGATCTCAAGATTTAAGTATTTGGGACAGATGGATATCGGAGAAAAAAGAAAAGCTCAGTTAGGTGCATTTACTTAT
TCTTTAGAAAAACAGGAAGTTCGTCTGCGGTTGTCTTCTGTAGGAGATTACCTTCAACGAGAGAGTTTAGTGATTCGGCT
ATTATACGGTACTTTTGAAACACCCCATCGTTGCTTTAGTTCTCGAGATTTTGCAACTATTTTACAGAAAACCGTCAGAC
GTGGATTATACCTTTTCAGCGGACCAGTCGGATCAGGCAAGACTTCTCTAATGTATCGGTTGGCTTTGGAAGAAAAATTG
CAAGTGATTACTATCGAAGATCCTGTAGAGATAGAAGAACCGTTTTTTCTGCAGCTACAGATCAATCCGAAAATAGCACA
GACTTATGATACACTTTTGAAACTCGCATTACGGCATCGTCCAGACTTGCTAATCATTGGCGAGATAAGAGATGAATCAA
CTGCACATGCTGCGGTAAGAGCAGCACTAACAGGGCACCGGGTTTTTGCAACCGTACATGCACGCAATCTTGAAGGTACA
TTGGTCAGAATCCATGAACTGACAAAGAAAAAAGAAGAGCTATCTGAATGTTTAGCTGGTGTCATTTATCAAGAACTTTT
GGAAGATGCCAATCACCATCCTTCTGTTTTATGGAGTTATCAATTTATGGAGGAAGAATCAGTAAAAAAATCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

47.635

100

0.485
  comYA Streptococcus mutans UA159

47.635

100

0.485
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.481

92.784

0.478
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

44.068

100

0.447
  comYA Streptococcus gordonii str. Challis substr. CH1

44.178

100

0.443
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

43.39

100

0.44
  comGA/cglA/cilD Streptococcus pneumoniae D39

43.39

100

0.44
  comGA/cglA/cilD Streptococcus pneumoniae R6

43.39

100

0.44
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

43.39

100

0.44
  comGA Lactococcus lactis subsp. cremoris KW2

45.185

92.784

0.419
  comGA Latilactobacillus sakei subsp. sakei 23K

42.126

87.285

0.368

Multiple sequence alignment