Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1P12_RS21025 Genome accession   NZ_CP091026
Coordinates   4193788..4195173 (+) Length   461 a.a.
NCBI ID   WP_096110959.1    Uniprot ID   -
Organism   Escherichia coli strain STEC383     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4188788..4200173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1P12_RS21000 aroP 4189336..4190709 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  L1P12_RS21005 ampE 4190752..4191606 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  L1P12_RS21010 ampD 4191603..4192154 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1P12_RS21015 nadC 4192242..4193135 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1P12_RS21020 pilA 4193338..4193778 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L1P12_RS21025 pilB 4193788..4195173 (+) 1386 WP_096110959.1 type II secretion system protein GspE Machinery gene
  L1P12_RS21030 hofC 4195163..4196365 (+) 1203 WP_000157262.1 protein transport protein HofC -
  L1P12_RS21035 guaC 4196400..4197443 (-) 1044 WP_001217338.1 GMP reductase -
  L1P12_RS21040 - 4197599..4197643 (-) 45 WP_120795372.1 protein YacM -
  L1P12_RS21045 coaE 4197668..4198288 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  L1P12_RS21050 zapD 4198288..4199031 (+) 744 WP_001194722.1 cell division protein ZapD -
  L1P12_RS21055 yacG 4199041..4199238 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  L1P12_RS21060 mutT 4199454..4199843 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50663.31 Da        Isoelectric Point: 6.4981

>NTDB_id=557735 L1P12_RS21025 WP_096110959.1 4193788..4195173(+) (pilB) [Escherichia coli strain STEC383]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGELIHIPDTVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=557735 L1P12_RS21025 WP_096110959.1 4193788..4195173(+) (pilB) [Escherichia coli strain STEC383]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CAGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGACGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGTGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCTCATCCATATTCCAGACACTGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.443

100

0.445
  pilB Legionella pneumophila strain ERS1305867

50.773

84.165

0.427
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Acinetobacter baylyi ADP1

39.44

100

0.397
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.753

83.514

0.39
  pilB Haemophilus influenzae 86-028NP

45.736

83.948

0.384
  pilB Haemophilus influenzae Rd KW20

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.846

84.599

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

41.542

87.202

0.362