Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L1P12_RS21020 Genome accession   NZ_CP091026
Coordinates   4193338..4193778 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain STEC383     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4188338..4198778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1P12_RS21000 aroP 4189336..4190709 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  L1P12_RS21005 ampE 4190752..4191606 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  L1P12_RS21010 ampD 4191603..4192154 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1P12_RS21015 nadC 4192242..4193135 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1P12_RS21020 pilA 4193338..4193778 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L1P12_RS21025 pilB 4193788..4195173 (+) 1386 WP_096110959.1 type II secretion system protein GspE Machinery gene
  L1P12_RS21030 hofC 4195163..4196365 (+) 1203 WP_000157262.1 protein transport protein HofC -
  L1P12_RS21035 guaC 4196400..4197443 (-) 1044 WP_001217338.1 GMP reductase -
  L1P12_RS21040 - 4197599..4197643 (-) 45 WP_120795372.1 protein YacM -
  L1P12_RS21045 coaE 4197668..4198288 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=557734 L1P12_RS21020 WP_000360904.1 4193338..4193778(+) (pilA) [Escherichia coli strain STEC383]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=557734 L1P12_RS21020 WP_000360904.1 4193338..4193778(+) (pilA) [Escherichia coli strain STEC383]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363