Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J7649_RS09130 Genome accession   NZ_CP072549
Coordinates   1917691..1919400 (+) Length   569 a.a.
NCBI ID   WP_219307524.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain H7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1912691..1924400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7649_RS09085 (J7649_09085) nusA 1913277..1914761 (-) 1485 WP_004281105.1 transcription termination factor NusA -
  J7649_RS09090 (J7649_09090) rimP 1914796..1915320 (-) 525 WP_004281103.1 ribosome maturation factor RimP -
  J7649_RS09120 (J7649_09120) secG 1916279..1916608 (-) 330 WP_219307520.1 preprotein translocase subunit SecG -
  J7649_RS09125 (J7649_09125) tpiA 1916622..1917413 (-) 792 WP_219307522.1 triose-phosphate isomerase -
  J7649_RS09130 (J7649_09130) pilB 1917691..1919400 (+) 1710 WP_219307524.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J7649_RS09135 (J7649_09135) pilC 1919432..1920661 (+) 1230 WP_219307525.1 type II secretion system F family protein Machinery gene
  J7649_RS16910 (J7649_09140) pilD 1920661..1921521 (+) 861 WP_219307528.1 prepilin peptidase Machinery gene
  J7649_RS09145 (J7649_09145) coaE 1921523..1922122 (+) 600 WP_219307530.1 dephospho-CoA kinase -
  J7649_RS09150 (J7649_09150) - 1922144..1923067 (-) 924 WP_004644926.1 DMT family transporter -
  J7649_RS09155 (J7649_09155) rlmB 1923074..1923823 (-) 750 WP_005101440.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J7649_RS09160 (J7649_09160) - 1923960..1924283 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63512.70 Da        Isoelectric Point: 4.8339

>NTDB_id=554127 J7649_RS09130 WP_219307524.1 1917691..1919400(+) (pilB) [Acinetobacter lwoffii strain H7]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKVDIVAELINQQQLSPTLIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=554127 J7649_RS09130 WP_219307524.1 1917691..1919400(+) (pilB) [Acinetobacter lwoffii strain H7]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCGTTGGCGCATGCCAAGCAGGAAAAAGTCGATATTGTGGCTGAGTTAATTAATCAGCAGCAGCTTTCTC
CGACCCTCATTGCTGAAACGATTTCAGTTGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCCCAAATT
TTGCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATCCTGCCGATTTTTAAAAATTCCAGTATTTTATATGT
TGCAACTAGCAACCCGACCAACATCGAAGCAATTGATGCAGTCCGTTTTTCGACTAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGAGTTTGATGAAGACTTTGAT
CTGGATGTTGATGTTGAATCTGCTGATCCAAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTGCTGATTGATGCGATCCGTATGGGCGCTTCAGATCTACACTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTCGATGGTGTTCTACGTCAGATTGCAACCCCGCCCTTACAGCTGGCAAATCGTTTGTCTTCACGT
CTTAAAGTTATGTCGCAAATGGACATTTCTGAAAAACGGGTCCCGCAAGATGGCCGAATCAAGCTAAAATTATCCAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACTCTGTTTGGCGAGAAGCTGGTACTGCGTATTCTGGATCCCTCCA
GCGCGATGCTCGGTATTGATGCACTGGGTTATGAACCGGAACAAAAAGAACTGTTTATGCAAGCACTGGACAAACCACAA
GGGATGCTGCTGATTACCGGCCCGACGGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTGCAAGGGATTAATCAGGTCAATGTCAACAATAAAG
TTGGTCTGACCTTCTCTGCCGCCCTGAAGTCCTTCTTACGTCAAGATCCGGATATCGTGATGGTCGGTGAGATTCGGGAT
CTGGAAACTGCAGAGATTGCGATTAAAGCGGCCCAGACCGGCCATATGGTGATGTCGACCTTACATACCAATAGTGCGCC
TGAGACTTTGACCCGTTTACGCAATATGGGGGTGCCTTCTTTCAATATTGCCACTTCAGTCAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGCCTGTGTTCACAGTGCAAAAAACCTGCCGATATTCCGAAACAAAGCTTATTGGAAATGGGCTTTACC
GAAACTGATCTGCAGCAACCAGAATTCCAAATCTATGAACCAGTAGGTTGCAATGAATGTCGTGAAGGCTATAAAGGTCG
TGTGGGTATTTATGAAGTCATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CCGATGCTTCCGCACGTGCAGGTTTTAATAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTAACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.702

100

0.808
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

49.016

98.243

0.482
  pilF Neisseria gonorrhoeae MS11

48.485

98.594

0.478
  pilB Vibrio campbellii strain DS40M4

48.387

98.067

0.475
  pilB Vibrio cholerae strain A1552

50.965

91.037

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

35.563

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.102

100

0.374
  pilF Thermus thermophilus HB27

37.345

99.297

0.371