Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J5X96_RS02970 Genome accession   NZ_CP072548
Coordinates   572591..573040 (-) Length   149 a.a.
NCBI ID   WP_209364314.1    Uniprot ID   -
Organism   Aggregatibacter sp. 2125159857     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 567591..578040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X96_RS02940 (J5X96_02940) - 568099..568365 (-) 267 WP_209364309.1 GNAT family N-acetyltransferase -
  J5X96_RS02945 (J5X96_02945) yacG 568365..568577 (-) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  J5X96_RS02950 (J5X96_02950) coaE 568570..569190 (-) 621 WP_209364310.1 dephospho-CoA kinase -
  J5X96_RS02955 (J5X96_02955) - 569243..569935 (-) 693 WP_209364311.1 A24 family peptidase -
  J5X96_RS02960 (J5X96_02960) pilC 569932..571170 (-) 1239 WP_209364312.1 type II secretion system F family protein Machinery gene
  J5X96_RS02965 (J5X96_02965) pilB 571163..572572 (-) 1410 WP_209364313.1 GspE/PulE family protein Machinery gene
  J5X96_RS02970 (J5X96_02970) pilA 572591..573040 (-) 450 WP_209364314.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5X96_RS02975 (J5X96_02975) ampD 573164..573718 (+) 555 WP_209364315.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J5X96_RS02980 (J5X96_02980) - 574320..574865 (+) 546 WP_033002478.1 carboxymuconolactone decarboxylase family protein -
  J5X96_RS02985 (J5X96_02985) rsuA 575040..575744 (+) 705 WP_209364316.1 16S rRNA pseudouridine(516) synthase RsuA -
  J5X96_RS02990 (J5X96_02990) - 575746..576942 (+) 1197 WP_033002479.1 Bcr/CflA family multidrug efflux MFS transporter -
  J5X96_RS02995 (J5X96_02995) ccmA 577135..577770 (+) 636 WP_209364755.1 cytochrome c biogenesis heme-transporting ATPase CcmA -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15543.85 Da        Isoelectric Point: 8.8121

>NTDB_id=554093 J5X96_RS02970 WP_209364314.1 572591..573040(-) (pilA) [Aggregatibacter sp. 2125159857]
MKSQSAFFSVKSMKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAVSELLQASAPLRAEVELCIYNTGKTEGCSGGQ
NGIQPDITNATQKYVKSTAVKNGAITVVGKSTLDNVTYTLTASGGTSNGVTWTASCGTNADIFPAGFCS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=554093 J5X96_RS02970 WP_209364314.1 572591..573040(-) (pilA) [Aggregatibacter sp. 2125159857]
ATGAAAAGTCAGTCCGCATTTTTCTCCGTGAAATCCATGAAAAAAGGGTTTACATTAATTGAATTAATGATCGTCATTGC
GATCGTGGCAATTTTAGCCACTATCGCCATTCCATCTTATCAAAACTACACCAAAAAAGCGGCAGTTTCCGAACTGTTGC
AAGCCTCTGCGCCACTTCGTGCTGAAGTGGAATTGTGCATTTATAACACCGGCAAAACAGAAGGCTGTAGTGGCGGACAA
AATGGCATTCAACCCGACATCACAAATGCCACACAAAAATATGTCAAATCCACGGCGGTTAAAAACGGTGCCATCACAGT
GGTCGGAAAAAGCACATTAGACAATGTTACTTATACCCTAACGGCATCCGGTGGCACCTCAAATGGCGTTACATGGACGG
CCTCTTGCGGCACTAACGCCGACATCTTCCCGGCAGGTTTTTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

67.626

93.289

0.631
  pilA Haemophilus influenzae Rd KW20

63.83

94.631

0.604
  pilA Glaesserella parasuis strain SC1401

53.147

95.973

0.51
  pilA2 Legionella pneumophila str. Paris

42.537

89.933

0.383
  pilA2 Legionella pneumophila strain ERS1305867

41.791

89.933

0.376
  pilA Vibrio campbellii strain DS40M4

37.671

97.987

0.369