Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J5X96_RS02965 Genome accession   NZ_CP072548
Coordinates   571163..572572 (-) Length   469 a.a.
NCBI ID   WP_209364313.1    Uniprot ID   -
Organism   Aggregatibacter sp. 2125159857     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 566163..577572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X96_RS02935 (J5X96_02935) - 566358..567731 (-) 1374 WP_209364308.1 sodium:alanine symporter family protein -
  J5X96_RS02940 (J5X96_02940) - 568099..568365 (-) 267 WP_209364309.1 GNAT family N-acetyltransferase -
  J5X96_RS02945 (J5X96_02945) yacG 568365..568577 (-) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  J5X96_RS02950 (J5X96_02950) coaE 568570..569190 (-) 621 WP_209364310.1 dephospho-CoA kinase -
  J5X96_RS02955 (J5X96_02955) - 569243..569935 (-) 693 WP_209364311.1 A24 family peptidase -
  J5X96_RS02960 (J5X96_02960) pilC 569932..571170 (-) 1239 WP_209364312.1 type II secretion system F family protein Machinery gene
  J5X96_RS02965 (J5X96_02965) pilB 571163..572572 (-) 1410 WP_209364313.1 GspE/PulE family protein Machinery gene
  J5X96_RS02970 (J5X96_02970) pilA 572591..573040 (-) 450 WP_209364314.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5X96_RS02975 (J5X96_02975) ampD 573164..573718 (+) 555 WP_209364315.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J5X96_RS02980 (J5X96_02980) - 574320..574865 (+) 546 WP_033002478.1 carboxymuconolactone decarboxylase family protein -
  J5X96_RS02985 (J5X96_02985) rsuA 575040..575744 (+) 705 WP_209364316.1 16S rRNA pseudouridine(516) synthase RsuA -
  J5X96_RS02990 (J5X96_02990) - 575746..576942 (+) 1197 WP_033002479.1 Bcr/CflA family multidrug efflux MFS transporter -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52892.34 Da        Isoelectric Point: 6.1354

>NTDB_id=554092 J5X96_RS02965 WP_209364313.1 571163..572572(-) (pilB) [Aggregatibacter sp. 2125159857]
MQHLVESSYTIRSQQGDLFTITPELWQQNQQQQSVLLRYLALPLKEEPQKLWLGLDSLANLAACEAFSFLTGKNIEPVLI
EPAVLKKALQDLVPHQEKLDENQPLFYSVTPQEQQEQFSDEPTIQLLNQIFENATTKKASDIHLEPQADFLQVRFRIDGV
LQVQNTIAQALANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLATHFGEKAVLRLQQNRPVQLAFSEL
GMTEQQQHTFRHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPEKHIMTAEDPIEIQLPGIIQTQVNPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQQHEIDSSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYQGRIGVYQFLQPDVSQTSGYQLDFQNLYASALEKVKSQVTDLAEVQRVLGQPNA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=554092 J5X96_RS02965 WP_209364313.1 571163..572572(-) (pilB) [Aggregatibacter sp. 2125159857]
ATGCAGCATTTGGTGGAATCTTCCTATACGATCCGTAGCCAACAAGGCGATCTCTTTACGATTACGCCCGAGTTATGGCA
ACAAAACCAACAGCAACAAAGCGTTCTTTTGCGTTATTTGGCGCTACCATTAAAAGAAGAACCACAAAAATTATGGCTAG
GGTTGGATTCCTTAGCCAACCTTGCCGCTTGTGAAGCGTTTTCCTTTTTAACCGGGAAAAATATTGAGCCGGTACTCATT
GAACCTGCCGTGTTAAAAAAGGCCTTGCAGGATTTAGTGCCACATCAAGAAAAACTCGATGAAAATCAACCGCTCTTTTA
TTCCGTCACGCCTCAGGAGCAACAAGAACAATTCTCCGATGAGCCAACGATTCAGTTGCTTAACCAAATTTTTGAAAACG
CCACCACCAAAAAAGCCTCCGATATTCATTTAGAACCGCAAGCGGATTTTTTACAAGTCCGTTTTCGTATTGACGGCGTA
TTGCAAGTGCAAAATACCATTGCGCAGGCGCTCGCCAACCGACTAATTTCCCGTTTGAAATTACTGGCGAAATTGGACAT
CAGCGAAACCCGCCTGCCGCAAGACGGACGGTTTCAGTTTAAAACGACGTTTTCCGATATTTTGGATTTTCGGCTCTCCA
CACTCGCGACGCATTTTGGTGAAAAAGCCGTCTTGCGTTTACAACAAAACCGCCCCGTACAGCTGGCTTTTAGTGAACTG
GGCATGACGGAACAACAGCAACACACCTTTCGTCATGCGCTCAGCCAACCGCAAGGGCTGATTTTAGTCACTGGTCCGAC
AGGAAGTGGCAAAAGTATTTCGTTATATACGGCATTACAATGGCTCAATACGCCCGAGAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAATTGCCGGGCATTATCCAAACCCAAGTCAATCCTCAAATCGGCTTAGATTTCAGTCGCTTGTTG
CGTACCTTTTTGCGTCAAGATCCCGACATTATTATGTTGGGTGAAATTCGTGACGAAGAAAGTGCCGCCATGGCTTTACG
GGCGGCGCAAACCGGCCATTTGGTATTATCGACGCTACATACCAATGATGCCCCTTCAGCTATCTCACGTTTACAACAAC
TTGGCATTCAACAACACGAAATCGATAGCAGTTTATTATTGGTGATCGCCCAACGCTTGGTGCGTAAACGATGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGTCATCAAGGCTACCAAGGTCGAATCGGTGTTTATCAATTCCTTCAGCC
TGATGTATCACAAACCTCAGGTTATCAATTGGATTTTCAGAATTTATATGCCAGCGCATTAGAAAAAGTGAAGTCGCAAG
TGACGGATTTAGCCGAAGTGCAACGGGTATTGGGGCAACCAAATGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

73.203

97.868

0.716
  pilB Haemophilus influenzae Rd KW20

72.767

97.868

0.712
  pilB Glaesserella parasuis strain SC1401

58.405

98.934

0.578
  pilB Acinetobacter baumannii D1279779

40.825

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

41.164

100

0.422
  pilB Vibrio campbellii strain DS40M4

40.541

100

0.416
  pilB Vibrio cholerae strain A1552

40.456

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.059

100

0.407
  pilB Acinetobacter baylyi ADP1

38

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

90.618

0.362