Detailed information NTG0554050

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: J7D37_RS16260
• Genome accession: NZ_CP072526
• Coordinates: 3408503..3409561 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain DETAB-E227
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3403503..3414561

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7D37_RS16235 (J7D37_16235)	aroK	3403817..3404386 (-)	570	WP_000643712.1	shikimate kinase AroK	-
J7D37_RS16240 (J7D37_16240)	pilQ	3404398..3406524 (-)	2127	WP_038347685.1	type IV pilus secretin PilQ	Machinery gene
J7D37_RS16245 (J7D37_16245)	pilP	3406587..3407114 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
J7D37_RS16250 (J7D37_16250)	pilO	3407125..3407865 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
J7D37_RS16255 (J7D37_16255)	pilN	3407862..3408503 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
J7D37_RS16260 (J7D37_16260)	pilM	3408503..3409561 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
J7D37_RS16265 (J7D37_16265)	ponA	3409723..3412278 (+)	2556	WP_038347682.1	penicillin-binding protein PBP1a	-
J7D37_RS16270 (J7D37_16270)	-	3412296..3413120 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
J7D37_RS16275 (J7D37_16275)	-	3413123..3413455 (-)	333	WP_038347679.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=554050 J7D37_RS16260 WP_002014066.1 3408503..3409561(-) (pilM) [Acinetobacter baumannii strain DETAB-E227]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=554050 J7D37_RS16260 WP_002014066.1 3408503..3409561(-) (pilM) [Acinetobacter baumannii strain DETAB-E227]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GGAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGCTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423