Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   J7445_RS00205 Genome accession   NZ_CP072524
Coordinates   44408..45649 (+) Length   413 a.a.
NCBI ID   WP_060974403.1    Uniprot ID   A0A1V0HD61
Organism   Neisseria sicca strain NS20201025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 39408..50649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS00190 (J7445_00190) - 40737..41834 (+) 1098 WP_070654842.1 YadA-like family protein -
  J7445_RS00195 (J7445_00195) bamE 41902..42369 (+) 468 WP_049228456.1 outer membrane protein assembly factor BamE -
  J7445_RS00200 (J7445_00200) pilF 42657..44336 (+) 1680 WP_049228453.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J7445_RS00205 (J7445_00205) pilG 44408..45649 (+) 1242 WP_060974403.1 type II secretion system F family protein Machinery gene
  J7445_RS00210 (J7445_00210) pilD 45651..46514 (+) 864 WP_070654844.1 A24 family peptidase Machinery gene
  J7445_RS00215 (J7445_00215) coaE 46516..47145 (+) 630 WP_019270097.1 dephospho-CoA kinase -
  J7445_RS00220 (J7445_00220) - 47302..47583 (+) 282 Protein_43 hypothetical protein -
  J7445_RS00225 (J7445_00225) yacG 47622..47816 (+) 195 WP_049228449.1 DNA gyrase inhibitor YacG -
  J7445_RS00230 (J7445_00230) ribF 47919..48848 (-) 930 WP_070654846.1 bifunctional riboflavin kinase/FAD synthetase -
  J7445_RS00235 (J7445_00235) - 48953..49345 (-) 393 WP_070654848.1 hypothetical protein -
  J7445_RS00240 (J7445_00240) ispH 49501..50469 (-) 969 WP_003756592.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45807.73 Da        Isoelectric Point: 9.9286

>NTDB_id=553890 J7445_RS00205 WP_060974403.1 44408..45649(+) (pilG) [Neisseria sicca strain NS20201025]
MAQAEQKRGLFAQKNKGKRFTFEGKNTNTDQLVRGEVVAKDEEEARKKLQRRGIRPLRISKVKATKKRRITQEDITVFTR
QLATMMKAGLPLMQAFEIVARGHSNPSMTEMLMQVRADVEQGSALGKSFAKYPKYFDRFYCNLIAAGETGGVLESLLDKL
AVYKEKTQAIKKKVKTALTYPISIVVVAVVLIFVMMRWVLPAFKEVYSNMGAELPGMTQTVMSISDFFVEYGWIMIIAAI
GIGFGVYKLHQKSPEFQKRVDAMVLRMPIFGSIIRKATIARWARTTSTLFAAGVPLVEVLDSVSGAAGNILYEEATQDIR
AKVTQGLSLTSSMQSTDMFPNMVIQMAAIGEESGSLDDMLNKAAEFYEEEVDNSVSQLSSLMEPIIMVVLGSIIGTILVA
MYLPLFNLGNVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=553890 J7445_RS00205 WP_060974403.1 44408..45649(+) (pilG) [Neisseria sicca strain NS20201025]
ATGGCTCAAGCAGAGCAAAAAAGAGGCTTATTCGCTCAAAAAAATAAAGGCAAACGTTTTACGTTTGAAGGTAAAAATAC
CAATACGGATCAATTAGTCCGAGGCGAAGTAGTCGCCAAAGACGAAGAAGAAGCGCGTAAAAAGCTCCAACGCCGCGGGA
TACGCCCTTTACGTATCAGTAAAGTAAAGGCAACCAAAAAGCGTCGTATTACTCAGGAAGACATTACAGTTTTCACCCGT
CAACTCGCCACCATGATGAAAGCAGGTCTGCCTTTAATGCAGGCTTTTGAAATTGTCGCACGCGGACACTCCAACCCATC
CATGACTGAAATGTTGATGCAAGTCCGTGCAGATGTGGAACAAGGTAGTGCATTAGGTAAATCATTTGCCAAATACCCAA
AATATTTCGACCGTTTTTATTGCAATCTGATTGCGGCTGGCGAGACCGGTGGTGTTTTAGAAAGCCTGTTGGATAAACTT
GCTGTATATAAAGAGAAAACACAAGCCATTAAGAAAAAAGTAAAAACTGCATTGACGTACCCAATTTCTATTGTGGTTGT
TGCGGTTGTACTGATTTTCGTAATGATGCGCTGGGTTTTGCCTGCATTTAAAGAGGTTTACTCCAATATGGGAGCAGAAT
TACCAGGTATGACACAAACAGTCATGAGTATTTCCGACTTTTTTGTGGAATATGGCTGGATCATGATTATTGCAGCAATT
GGCATTGGATTTGGTGTATATAAATTGCATCAGAAATCTCCTGAATTTCAAAAACGTGTAGATGCCATGGTTTTACGTAT
GCCTATTTTTGGCTCTATCATTCGTAAAGCAACTATTGCACGTTGGGCTCGTACTACTTCTACCTTATTTGCTGCCGGTG
TCCCTTTGGTTGAGGTTTTAGATTCTGTATCAGGTGCTGCCGGCAATATACTCTATGAAGAAGCAACGCAAGACATCCGT
GCAAAAGTTACTCAAGGCCTCTCTTTGACTTCAAGTATGCAAAGTACGGATATGTTCCCGAATATGGTTATTCAAATGGC
TGCTATTGGTGAAGAGTCAGGTTCGTTAGATGATATGTTAAATAAAGCTGCAGAGTTTTATGAGGAAGAAGTTGATAACT
CTGTATCACAACTATCTTCTTTGATGGAGCCGATTATCATGGTGGTTTTGGGCTCCATTATCGGTACAATCCTCGTGGCG
ATGTATCTGCCGCTGTTTAACCTCGGTAACGTAGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0HD61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

71.921

98.305

0.707
  pilG Neisseria meningitidis 44/76-A

71.429

98.305

0.702
  pilC Legionella pneumophila strain ERS1305867

46.173

98.063

0.453
  pilC Pseudomonas stutzeri DSM 10701

43.704

98.063

0.429
  pilC Acinetobacter baylyi ADP1

44.361

96.61

0.429
  pilC Acinetobacter baumannii D1279779

44.472

96.368

0.429
  pilC Vibrio cholerae strain A1552

38

96.852

0.368