Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J7445_RS00200 Genome accession   NZ_CP072524
Coordinates   42657..44336 (+) Length   559 a.a.
NCBI ID   WP_049228453.1    Uniprot ID   A0A2I1XCD3
Organism   Neisseria sicca strain NS20201025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 37657..49336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS00180 (J7445_00180) - 38278..38502 (+) 225 WP_209283081.1 glycine zipper 2TM domain-containing protein -
  J7445_RS00185 (J7445_00185) waaA 38650..39912 (-) 1263 WP_070654840.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  J7445_RS00190 (J7445_00190) - 40737..41834 (+) 1098 WP_070654842.1 YadA-like family protein -
  J7445_RS00195 (J7445_00195) bamE 41902..42369 (+) 468 WP_049228456.1 outer membrane protein assembly factor BamE -
  J7445_RS00200 (J7445_00200) pilF 42657..44336 (+) 1680 WP_049228453.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J7445_RS00205 (J7445_00205) pilG 44408..45649 (+) 1242 WP_060974403.1 type II secretion system F family protein Machinery gene
  J7445_RS00210 (J7445_00210) pilD 45651..46514 (+) 864 WP_070654844.1 A24 family peptidase Machinery gene
  J7445_RS00215 (J7445_00215) coaE 46516..47145 (+) 630 WP_019270097.1 dephospho-CoA kinase -
  J7445_RS00220 (J7445_00220) - 47302..47583 (+) 282 Protein_43 hypothetical protein -
  J7445_RS00225 (J7445_00225) yacG 47622..47816 (+) 195 WP_049228449.1 DNA gyrase inhibitor YacG -
  J7445_RS00230 (J7445_00230) ribF 47919..48848 (-) 930 WP_070654846.1 bifunctional riboflavin kinase/FAD synthetase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62379.94 Da        Isoelectric Point: 6.3585

>NTDB_id=553889 J7445_RS00200 WP_049228453.1 42657..44336(+) (pilF) [Neisseria sicca strain NS20201025]
MSIGLLRVLVQSQTINSTQAEHYNNILKTGQEILPMLFADKIISPRSLGELVARVFSYPLLDLHYYPRNNIVMDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQSFQKIAFASGVTVDLVVVPDDQLSSLLEWLGQRSTTILKEISEEQEAAQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKREVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKAMQGLTSLEEVIAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=553889 J7445_RS00200 WP_049228453.1 42657..44336(+) (pilF) [Neisseria sicca strain NS20201025]
ATGAGTATCGGATTATTACGAGTTTTAGTTCAAAGTCAGACAATCAACAGTACCCAGGCAGAGCATTACAACAATATATT
GAAAACGGGGCAGGAAATCTTGCCTATGCTTTTTGCGGATAAGATTATTAGCCCAAGGTCTTTGGGTGAGTTGGTTGCGC
GAGTTTTTAGTTATCCACTCTTGGATTTACATTATTATCCACGTAACAATATTGTTATGGATGTTTTGACCGAAGAACAG
ATGGTTCAGAATCGCTGTGTTCCGATTTTTCGTCGCGGTCGAAAGGTATATTTAGCGGTTTCAGATCCTACTCAAATCCA
AAGTTTTCAAAAAATAGCTTTTGCTTCAGGAGTGACAGTTGATTTGGTTGTCGTACCTGACGATCAATTAAGTTCCCTTC
TAGAATGGTTAGGACAACGTTCTACTACCATCTTAAAAGAAATTAGTGAAGAACAAGAAGCTGCTCAACCCTCACAAGCT
CTCTACATTGACAATGAAGAAGCTGAAGACGGTCCTATCCCTCGTTTTATTCACAAAACTTTATCGGATGCACTAAACGC
AGGCGCATCTGATATACATTTTGAATTTTACGAACAAATGGCTCGTGTTCGTTTTCGCGTAGACGGTCAGCTAAGAGAAG
TGGTTCAACCGCCTGTTGCAGTACGCGGACAGCTGGCTTCACGTATTAAAGTAATGGCGCGTTTGGATATTTCTGAAAAA
CGTGTGCCACAAGATGGTCGCATTCAGATTGCTTTCCATAAACATGGTCGCCCCATTGATTTCCGCGTGAGTACGCTACC
TACCCTTTTTGGTGAAAAAGTGGTAATGCGTATTCTGAATTCTGATGCGGCAACTTTAAATATTGACCAGTTGGGATTCG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATTCATCGCCCTTATGGCATGGTATTGGTAACAGGTCCGACAGGTTCA
GGTAAAACCGTATCGCTTTATACCTGTCTGAATATTTTAAATACAGAGGACGTCAATATTTCCACCGCAGAAGACCCTGC
AGAGATTAATTTACCAGGCATCAACCAAGTCAACGTCAATGATAAACAAGGTTTGACTTTTGCTGCAGCATTAAAATCTT
TCTTGCGTCAAGACCCTGACATCATCATGGTAGGTGAGATTCGTGATTTGGAAACTGCGGATATTGCCATCAAAGCGGCA
CAAACCGGACATATGGTGTTTTCAACGTTGCATACCAATAACGCGCCTGCGACCCTATCCCGTATGTTGAACATGGGGGT
TGCTCCATTCAACATTGCCAGTTCTGTCAGCTTAATTATGGCACAACGACTATTGCGTCGTCTGTGTTCGAGCTGTAAGC
GTGAAGTTGAGCGCCCCCCTGTTCCCGCATTGAAAAAAGCAGGATTTACAGATGAAGATTTAGCAAAGGATTGGAAACTC
TACCGTCCTGTCGGCTGCGATAGTTGCCGTGGTAAAGGCTTCAAAGGCCGTGTCGGCGTCTACGAAGTGATGCCGATTAC
CGAGGAAATGCAACGTGTCATTATGAATAATGGTACGGAAGTTGATATTATGAACATGGCCTATAAAGAAGGCATGGTCG
ATTTACGCCGCGCAGGTTTGTTAAAAGCTATGCAAGGTTTGACTTCATTAGAAGAAGTTATTGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I1XCD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

80.322

100

0.803
  pilB Acinetobacter baylyi ADP1

51.06

100

0.517
  pilB Acinetobacter baumannii D1279779

51.259

99.463

0.51
  pilB Legionella pneumophila strain ERS1305867

48.214

100

0.483
  pilB Vibrio cholerae strain A1552

45.907

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

44.858

100

0.453
  pilB Vibrio campbellii strain DS40M4

43.262

100

0.436
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.575

92.665

0.367