Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LRP95_RS23870 Genome accession   NZ_CP089745
Coordinates   5143307..5143774 (-) Length   155 a.a.
NCBI ID   WP_232683417.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Pa608     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5138307..5148774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRP95_RS23855 nadC 5140807..5141655 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LRP95_RS23865 - 5141836..5143233 (-) 1398 WP_014603302.1 O-antigen ligase family protein -
  LRP95_RS23870 pilA/pilAI 5143307..5143774 (-) 468 WP_232683417.1 pilin Machinery gene
  LRP95_RS23875 pilB 5144005..5145705 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LRP95_RS23880 pilC 5145709..5146926 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  LRP95_RS23885 pilD 5146927..5147799 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LRP95_RS23890 coaE 5147796..5148407 (+) 612 WP_014603303.1 dephospho-CoA kinase -
  LRP95_RS23895 yacG 5148404..5148604 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15943.34 Da        Isoelectric Point: 9.4382

>NTDB_id=552349 LRP95_RS23870 WP_232683417.1 5143307..5143774(-) (pilA/pilAI) [Pseudomonas aeruginosa strain Pa608]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQVYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=552349 LRP95_RS23870 WP_232683417.1 5143307..5143774(-) (pilA/pilAI) [Pseudomonas aeruginosa strain Pa608]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGTCTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.062

100

0.471
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.439
  pilA Acinetobacter baumannii strain A118

43.312

100

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

36.842

100

0.406
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.262

90.968

0.394
  pilA Vibrio cholerae strain A1552

43.262

90.968

0.394
  pilA Vibrio cholerae C6706

43.262

90.968

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.333

96.774

0.381