Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J5N61_RS16860 Genome accession   NZ_CP071973
Coordinates   3524883..3526058 (+) Length   391 a.a.
NCBI ID   WP_005403993.1    Uniprot ID   D0SRI2
Organism   Acinetobacter junii strain INC8271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3519883..3531058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5N61_RS16835 (J5N61_16835) - 3520546..3521019 (-) 474 WP_004914520.1 hypothetical protein -
  J5N61_RS16840 (J5N61_16840) guaD 3521205..3522530 (+) 1326 WP_151834399.1 guanine deaminase -
  J5N61_RS16845 (J5N61_16845) hpt 3522747..3523274 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  J5N61_RS16850 (J5N61_16850) - 3523503..3523754 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  J5N61_RS16855 (J5N61_16855) - 3523804..3524502 (-) 699 WP_005403992.1 DsbC family protein -
  J5N61_RS16860 (J5N61_16860) pilU 3524883..3526058 (+) 1176 WP_005403993.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J5N61_RS16865 (J5N61_16865) mnmE 3526114..3527469 (-) 1356 WP_039909157.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  J5N61_RS16870 (J5N61_16870) yidC 3527560..3529317 (-) 1758 WP_004914555.1 membrane protein insertase YidC -
  J5N61_RS16875 (J5N61_16875) yidD 3529323..3529643 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  J5N61_RS16880 (J5N61_16880) rnpA 3529653..3530048 (-) 396 WP_004950005.1 ribonuclease P protein component -
  J5N61_RS16885 (J5N61_16885) rpmH 3530077..3530211 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44349.87 Da        Isoelectric Point: 6.2400

>NTDB_id=549567 J5N61_RS16860 WP_005403993.1 3524883..3526058(+) (pilU) [Acinetobacter junii strain INC8271]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEYAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=549567 J5N61_RS16860 WP_005403993.1 3524883..3526058(+) (pilU) [Acinetobacter junii strain INC8271]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCCCTTA
CGGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTTTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCACGGTTTCGGGTCAATGTATTTAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTTAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GTGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGGAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAATATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTACGAACTCAAGATGGTCAAGGACGTCGAGCTGCCATTGAAA
TCCTCCTCAATACGCCATTGATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGTGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTGTATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAATTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0SRI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.923

93.606

0.542
  pilU Acinetobacter baylyi ADP1

55.908

88.747

0.496
  pilU Vibrio cholerae strain A1552

52.89

88.491

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366