Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   J4Q30_RS10510 Genome accession   NZ_CP071917
Coordinates   1990668..1991609 (-) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 19A-19339     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1948507..1999921 1990668..1991609 within 0


Gene organization within MGE regions


Location: 1948507..1999921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4Q30_RS10205 (J4Q30_10200) - 1948507..1949463 (-) 957 WP_233923013.1 N-acetylmuramoyl-L-alanine amidase family protein -
  J4Q30_RS10210 (J4Q30_10205) - 1949466..1949801 (-) 336 WP_050200954.1 phage holin -
  J4Q30_RS10215 (J4Q30_10210) - 1949805..1950221 (-) 417 WP_001165344.1 phage holin family protein -
  J4Q30_RS10220 (J4Q30_10215) - 1950231..1950581 (-) 351 WP_050200952.1 hypothetical protein -
  J4Q30_RS10225 (J4Q30_10220) - 1950584..1950787 (-) 204 WP_001091109.1 hypothetical protein -
  J4Q30_RS11785 - 1950768..1950884 (-) 117 WP_001063633.1 hypothetical protein -
  J4Q30_RS10230 (J4Q30_10225) - 1950881..1960636 (-) 9756 WP_408669604.1 tail fiber domain-containing protein -
  J4Q30_RS10235 (J4Q30_10235) - 1960677..1961027 (-) 351 WP_000068025.1 DUF6711 family protein -
  J4Q30_RS10240 (J4Q30_10240) - 1961036..1964689 (-) 3654 WP_233922964.1 hypothetical protein -
  J4Q30_RS10245 (J4Q30_10245) - 1964676..1965026 (-) 351 WP_000478016.1 hypothetical protein -
  J4Q30_RS10250 (J4Q30_10250) - 1965065..1965445 (-) 381 WP_001185632.1 DUF6096 family protein -
  J4Q30_RS10255 (J4Q30_10255) - 1965450..1965863 (-) 414 WP_000880676.1 phage tail tube protein -
  J4Q30_RS10260 (J4Q30_10260) - 1965866..1966234 (-) 369 WP_000608235.1 hypothetical protein -
  J4Q30_RS10265 (J4Q30_10265) - 1966231..1966746 (-) 516 WP_044812726.1 HK97-gp10 family putative phage morphogenesis protein -
  J4Q30_RS10270 (J4Q30_10270) - 1966721..1967059 (-) 339 WP_000478943.1 hypothetical protein -
  J4Q30_RS10275 (J4Q30_10275) - 1967040..1967351 (-) 312 WP_000021221.1 phage head-tail connector protein -
  J4Q30_RS10280 (J4Q30_10280) - 1967353..1967541 (-) 189 WP_000669348.1 hypothetical protein -
  J4Q30_RS10285 (J4Q30_10285) - 1967531..1967713 (-) 183 WP_000054934.1 Rho termination factor N-terminal domain-containing protein -
  J4Q30_RS10290 (J4Q30_10290) - 1967725..1968570 (-) 846 WP_000123890.1 N4-gp56 family major capsid protein -
  J4Q30_RS10295 (J4Q30_10295) - 1968577..1969161 (-) 585 WP_001288026.1 DUF4355 domain-containing protein -
  J4Q30_RS10300 (J4Q30_10300) - 1969378..1969629 (-) 252 WP_000890163.1 DUF6275 family protein -
  J4Q30_RS10305 (J4Q30_10305) - 1969631..1969876 (-) 246 WP_050221455.1 hypothetical protein -
  J4Q30_RS10310 (J4Q30_10310) - 1969928..1970155 (-) 228 WP_050110656.1 hypothetical protein -
  J4Q30_RS10315 (J4Q30_10315) - 1970143..1971546 (-) 1404 WP_225791470.1 minor capsid protein -
  J4Q30_RS10320 (J4Q30_10320) - 1971455..1972924 (-) 1470 WP_078730733.1 phage portal protein -
  J4Q30_RS10325 (J4Q30_10325) - 1972936..1974234 (-) 1299 WP_000084426.1 PBSX family phage terminase large subunit -
  J4Q30_RS10330 (J4Q30_10330) - 1974212..1974652 (-) 441 WP_001859583.1 terminase small subunit -
  J4Q30_RS10340 (J4Q30_10340) - 1975126..1975548 (-) 423 WP_001030244.1 DUF1492 domain-containing protein -
  J4Q30_RS10345 (J4Q30_10345) - 1975618..1975983 (-) 366 WP_000802874.1 hypothetical protein -
  J4Q30_RS10350 (J4Q30_10350) - 1975980..1976300 (-) 321 WP_320408135.1 3-dehydroquinate synthase -
  J4Q30_RS10355 (J4Q30_10355) - 1976556..1976735 (-) 180 WP_001042650.1 hypothetical protein -
  J4Q30_RS10360 (J4Q30_10360) - 1976728..1977222 (-) 495 WP_233922966.1 YopX family protein -
  J4Q30_RS10365 (J4Q30_10365) - 1977219..1977737 (-) 519 WP_233922967.1 DUF1642 domain-containing protein -
  J4Q30_RS10370 (J4Q30_10370) - 1977739..1978056 (-) 318 WP_174222359.1 hypothetical protein -
  J4Q30_RS10375 (J4Q30_10375) - 1978081..1978263 (-) 183 WP_000796349.1 hypothetical protein -
  J4Q30_RS10380 (J4Q30_10380) - 1978279..1978710 (-) 432 WP_000779143.1 RusA family crossover junction endodeoxyribonuclease -
  J4Q30_RS10385 (J4Q30_10385) - 1978707..1979036 (-) 330 WP_050210841.1 hypothetical protein -
  J4Q30_RS10390 (J4Q30_10390) - 1979050..1979259 (-) 210 WP_000455269.1 hypothetical protein -
  J4Q30_RS10395 (J4Q30_10395) - 1979261..1979956 (-) 696 WP_050099130.1 site-specific DNA-methyltransferase -
  J4Q30_RS10400 (J4Q30_10400) ssbA 1979969..1980385 (-) 417 WP_050201984.1 single-stranded DNA-binding protein Machinery gene
  J4Q30_RS10405 (J4Q30_10405) - 1980375..1980518 (-) 144 WP_153277088.1 hypothetical protein -
  J4Q30_RS10410 (J4Q30_10410) - 1980521..1981585 (-) 1065 WP_233922968.1 DUF1351 domain-containing protein -
  J4Q30_RS10415 (J4Q30_10415) bet 1981595..1982347 (-) 753 WP_050263779.1 phage recombination protein Bet -
  J4Q30_RS10420 (J4Q30_10420) - 1982365..1982550 (-) 186 WP_000746960.1 hypothetical protein -
  J4Q30_RS10425 (J4Q30_10425) - 1982719..1982862 (-) 144 WP_001862963.1 hypothetical protein -
  J4Q30_RS10430 (J4Q30_10430) - 1982849..1983109 (-) 261 WP_000471463.1 hypothetical protein -
  J4Q30_RS10435 (J4Q30_10435) - 1983122..1983376 (-) 255 WP_000275521.1 hypothetical protein -
  J4Q30_RS10440 (J4Q30_10440) - 1983377..1983598 (-) 222 WP_001864263.1 hypothetical protein -
  J4Q30_RS10445 (J4Q30_10445) - 1983598..1983759 (-) 162 WP_000823399.1 BOW99_gp33 family protein -
  J4Q30_RS10450 (J4Q30_10450) - 1983824..1983967 (-) 144 WP_001862958.1 hypothetical protein -
  J4Q30_RS10455 (J4Q30_10455) - 1984084..1984308 (+) 225 WP_000517704.1 DUF2188 domain-containing protein -
  J4Q30_RS10460 (J4Q30_10460) - 1984305..1984487 (-) 183 WP_001247797.1 hypothetical protein -
  J4Q30_RS10465 (J4Q30_10465) - 1984669..1984947 (-) 279 WP_000261154.1 HTH domain-containing protein -
  J4Q30_RS10470 (J4Q30_10470) - 1985114..1985350 (-) 237 WP_001157069.1 hypothetical protein -
  J4Q30_RS11625 - 1985424..1985549 (+) 126 WP_257885839.1 hypothetical protein -
  J4Q30_RS10475 (J4Q30_10480) - 1985542..1985841 (-) 300 WP_191855094.1 hypothetical protein -
  J4Q30_RS10480 (J4Q30_10485) - 1985916..1986191 (-) 276 WP_050202804.1 hypothetical protein -
  J4Q30_RS10485 (J4Q30_10490) - 1986352..1987104 (+) 753 WP_233922969.1 XRE family transcriptional regulator -
  J4Q30_RS10490 (J4Q30_10495) - 1987106..1987870 (+) 765 WP_219576745.1 hypothetical protein -
  J4Q30_RS10495 (J4Q30_10500) - 1988177..1989622 (+) 1446 WP_219576746.1 recombinase family protein -
  J4Q30_RS10505 (J4Q30_10510) comGB/cglB 1989704..1990720 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  J4Q30_RS10510 (J4Q30_10515) comGA/cglA/cilD 1990668..1991609 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  J4Q30_RS10515 (J4Q30_10520) - 1991685..1992050 (-) 366 WP_000286415.1 DUF1033 family protein -
  J4Q30_RS10520 (J4Q30_10525) - 1992201..1993259 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  J4Q30_RS10525 (J4Q30_10530) nagA 1993422..1994573 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  J4Q30_RS10530 (J4Q30_10535) - 1994726..1996543 (-) 1818 WP_001220865.1 acyltransferase family protein -
  J4Q30_RS10535 (J4Q30_10540) tgt 1996644..1997786 (-) 1143 WP_001285241.1 tRNA guanosine(34) transglycosylase Tgt -
  J4Q30_RS10540 (J4Q30_10545) - 1997916..1998773 (+) 858 WP_001108863.1 DUF975 family protein -
  J4Q30_RS10545 (J4Q30_10550) pcp 1998801..1999445 (-) 645 WP_000866916.1 pyroglutamyl-peptidase I -
  J4Q30_RS10550 (J4Q30_10555) - 1999520..1999921 (-) 402 WP_000022864.1 DUF1304 domain-containing protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=549064 J4Q30_RS10510 WP_000249567.1 1990668..1991609(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 19A-19339]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=549064 J4Q30_RS10510 WP_000249567.1 1990668..1991609(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 19A-19339]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGGCTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCTAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTTTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361