Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J4N39_RS12300 Genome accession   NZ_CP071848
Coordinates   2612476..2612889 (+) Length   137 a.a.
NCBI ID   WP_252019839.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43136     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2607476..2617889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N39_RS12285 (J4N39_12295) pdhR 2609606..2610373 (-) 768 WP_252019833.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  J4N39_RS12290 (J4N39_12300) ampD 2610667..2611212 (-) 546 WP_252019835.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J4N39_RS12295 (J4N39_12305) nadC 2611373..2612260 (+) 888 WP_252019837.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J4N39_RS12300 (J4N39_12310) pilA 2612476..2612889 (+) 414 WP_252019839.1 pilin Machinery gene
  J4N39_RS12305 (J4N39_12315) pilB 2612893..2614569 (+) 1677 WP_252019841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4N39_RS12310 (J4N39_12320) pilC 2614577..2615800 (+) 1224 WP_252019849.1 type II secretion system F family protein Machinery gene
  J4N39_RS12315 (J4N39_12325) pilD 2615831..2616706 (+) 876 WP_252019851.1 A24 family peptidase Machinery gene
  J4N39_RS12320 (J4N39_12330) coaE 2616709..2617314 (+) 606 WP_252019857.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14166.09 Da        Isoelectric Point: 6.2022

>NTDB_id=548299 J4N39_RS12300 WP_252019839.1 2612476..2612889(+) (pilA) [Vibrio sp. SCSIO 43136]
MKKQQGFTLIELMIVVAVIGVLAMVAVPQYQKYVGKSEAASALASITGLRTNVETYVIDNGAFPANSAAVAIPTITGATI
SFQSISGAGGTIQYLFSSSGNSANVNGKKVNLIRTGDGNWSCSSDINDTDIEPKGCS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=548299 J4N39_RS12300 WP_252019839.1 2612476..2612889(+) (pilA) [Vibrio sp. SCSIO 43136]
ATGAAAAAACAACAGGGCTTTACACTGATTGAATTGATGATTGTTGTGGCGGTGATTGGCGTATTGGCGATGGTAGCTGT
ACCGCAATATCAAAAATACGTTGGCAAATCAGAAGCAGCTAGTGCTTTAGCTTCTATTACCGGCCTAAGAACAAATGTTG
AAACTTATGTAATTGATAACGGAGCATTTCCAGCAAACTCTGCCGCTGTAGCGATTCCAACCATTACAGGGGCAACAATT
TCATTTCAAAGCATCAGCGGCGCTGGGGGCACAATTCAATATCTGTTCAGCAGCTCTGGCAATAGTGCAAATGTTAACGG
TAAAAAAGTTAACCTAATTAGAACTGGTGATGGGAACTGGAGCTGTAGCTCTGATATCAATGACACTGACATTGAGCCTA
AAGGTTGTAGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.575

100

0.496
  pilA Vibrio cholerae strain A1552

46.575

100

0.496
  pilA Vibrio cholerae C6706

46.575

100

0.496
  pilA Pseudomonas aeruginosa PAK

42.667

100

0.467
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.754

100

0.431
  pilA Acinetobacter baumannii strain A118

40

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

98.54

0.394