Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J2887_RS14205 Genome accession   NZ_CP071785
Coordinates   3121568..3123286 (+) Length   572 a.a.
NCBI ID   WP_227171759.1    Uniprot ID   -
Organism   Marinobacter sp. CA1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3116568..3128286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2887_RS14180 (J2887_14145) - 3116912..3117796 (-) 885 WP_227171757.1 glycosyltransferase family 2 protein -
  J2887_RS14185 (J2887_14150) - 3117833..3118687 (-) 855 WP_227176133.1 glycosyltransferase family 2 protein -
  J2887_RS14190 (J2887_14155) - 3118733..3120658 (-) 1926 WP_227171758.1 hypothetical protein -
  J2887_RS14195 (J2887_14160) - 3120743..3121231 (-) 489 WP_324197216.1 pilin -
  J2887_RS14205 (J2887_14165) pilB 3121568..3123286 (+) 1719 WP_227171759.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J2887_RS14210 (J2887_14170) pilC 3123289..3124506 (+) 1218 WP_227171760.1 type II secretion system F family protein Machinery gene
  J2887_RS14215 (J2887_14175) pilD 3124513..3125382 (+) 870 WP_227171761.1 prepilin peptidase Machinery gene
  J2887_RS14220 (J2887_14180) coaE 3125379..3125996 (+) 618 WP_227171762.1 dephospho-CoA kinase -
  J2887_RS14225 (J2887_14185) - 3125974..3127128 (+) 1155 WP_227171763.1 hypothetical protein -
  J2887_RS14230 (J2887_14190) tsaA 3127125..3127961 (+) 837 WP_227171764.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62942.79 Da        Isoelectric Point: 4.6625

>NTDB_id=547758 J2887_RS14205 WP_227171759.1 3121568..3123286(+) (pilB) [Marinobacter sp. CA1]
MATSTRNITLTGLARRFVDDELIDEEMAKEAYLQASENKIPLISYLVQNDLADSQGLAFSAAMEFGVSVLDLEAFAPEMM
PDKLVDEKLIRKHSALPLYRRGNRLFIAVSDPTNIQALDEIKFNTGLSTDPILVDDQKLRNAIERFLESQDTTMGDLEDS
DLEAIDTDGGESDDEEGATSTSEVDDAPIVKYVNKLLLDAIRTGASDIHFEPYEKVYRVRYRTDGILKEVSKPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEGQKQLYLDAL
EQPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDIIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSNCKEPADVPKEALLA
EGFTEAQIETGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDKLANMIMEEASSIAIAKQAHEEGFANLRQSALEKVIQGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=547758 J2887_RS14205 WP_227171759.1 3121568..3123286(+) (pilB) [Marinobacter sp. CA1]
ATGGCTACTAGCACTCGCAACATCACCCTCACAGGCCTCGCCCGTCGCTTCGTAGACGACGAATTGATCGATGAGGAAAT
GGCCAAAGAGGCCTATCTGCAGGCCTCCGAGAACAAGATCCCGCTAATTTCCTACCTGGTGCAAAACGATCTCGCTGACA
GCCAAGGGCTTGCGTTTTCGGCAGCCATGGAGTTTGGGGTATCCGTCCTGGACCTAGAGGCATTCGCGCCAGAGATGATG
CCCGACAAGCTAGTTGACGAAAAGCTCATTAGAAAGCACAGCGCCCTCCCCTTGTACCGACGGGGTAATCGGCTCTTCAT
TGCGGTTTCTGATCCCACCAACATCCAGGCACTTGATGAAATCAAGTTCAACACGGGCCTCAGTACTGATCCCATTCTGG
TAGATGACCAGAAGCTCCGCAATGCCATTGAGCGCTTCCTTGAGTCTCAAGACACCACAATGGGAGACTTGGAGGATTCG
GACCTAGAGGCAATAGACACCGATGGCGGAGAGAGTGACGATGAGGAAGGAGCTACCTCAACGTCCGAGGTTGACGATGC
ACCTATTGTCAAATACGTAAACAAGCTCCTGCTTGATGCCATTCGCACCGGCGCCTCTGATATCCACTTTGAACCCTATG
AAAAGGTATACCGAGTGCGGTATCGCACCGATGGCATTCTGAAAGAAGTTTCAAAGCCTTCGATCAAGCTGGCGCCGAAG
ATATCTGCACGGGTGAAGATTATGGCCCAGTTGGATATTTCGGAACGCAGGGTTCCTCAGGATGGCCGCATCAAGATGAA
GCTATCCAAAACGAAGGCCATCGATTTCCGAGTCAACACCCTGCCAACACTCTGGGGCGAGAAAATCGTGCTTCGGATTC
TTGACCCCAGCCAGGCCAAACTCGGTATCGACGTGCTTGGTTACGAAGAGGGTCAAAAGCAGCTGTACCTGGATGCTCTC
GAGCAACCTCAGGGAATGATTCTTGTCACGGGCCCCACAGGGTCCGGCAAAACGGTTTCTCTGTACACCGGCCTCAACAT
TCTCAACACAGCCGAGCGCAACATTTCGACAGCGGAAGATCCCGCCGAGATCAATCTTGAAGGCATCAATCAGGTGAACG
TGAACACCAAGGTTGGCCTTGGTTTTGCCGAAGCGCTCCGAGCATTCCTCCGACAAGACCCGGACATCATCATGGTTGGG
GAGATCCGAGATCTCGAAACCGCCAATATCGCCATTAAAGCGGCTCAAACCGGTCACCTGGTACTTTCTACTCTCCACAC
CAACAGTGCCGCAGAAACGCTTACCCGGATGATGAACATGGGGGTTCCTTCGTTCAACATTGCGACATCCGTCAGCCTGA
TTATTGCACAGCGACTAGGTCGCCGGCTCTGTAGCAATTGCAAAGAACCCGCAGATGTCCCCAAGGAAGCACTTCTAGCG
GAGGGGTTCACAGAGGCACAAATTGAAACAGGCTTCACGTTGTACCGCCCTAAAGGCTGTGATAAATGCAATGGAGGCTA
TAAGGGCCGCGTCGGTATCTACGAGGTGGTCAAGATCACAGACAAGCTGGCGAACATGATCATGGAGGAGGCAAGCTCCA
TCGCGATTGCCAAGCAAGCGCACGAGGAAGGCTTTGCGAACTTGCGGCAGTCGGCGTTGGAGAAAGTCATTCAGGGGGTC
ACCAGCCTTGAAGAAGCGAACCGAGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.657

98.951

0.58
  pilB Acinetobacter baylyi ADP1

57.317

100

0.575
  pilB Legionella pneumophila strain ERS1305867

54.514

100

0.549
  pilB Vibrio cholerae strain A1552

51.67

99.476

0.514
  pilB Vibrio campbellii strain DS40M4

52.778

94.406

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

52.486

94.93

0.498
  pilF Neisseria gonorrhoeae MS11

48.239

99.301

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.987

92.133

0.378
  pilF Thermus thermophilus HB27

39.848

92.133

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

90.734

0.367