Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J2887_RS14215 Genome accession   NZ_CP071785
Coordinates   3124513..3125382 (+) Length   289 a.a.
NCBI ID   WP_227171761.1    Uniprot ID   -
Organism   Marinobacter sp. CA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3119513..3130382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2887_RS14195 (J2887_14160) - 3120743..3121231 (-) 489 WP_324197216.1 pilin -
  J2887_RS14205 (J2887_14165) pilB 3121568..3123286 (+) 1719 WP_227171759.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J2887_RS14210 (J2887_14170) pilC 3123289..3124506 (+) 1218 WP_227171760.1 type II secretion system F family protein Machinery gene
  J2887_RS14215 (J2887_14175) pilD 3124513..3125382 (+) 870 WP_227171761.1 prepilin peptidase Machinery gene
  J2887_RS14220 (J2887_14180) coaE 3125379..3125996 (+) 618 WP_227171762.1 dephospho-CoA kinase -
  J2887_RS14225 (J2887_14185) - 3125974..3127128 (+) 1155 WP_227171763.1 hypothetical protein -
  J2887_RS14230 (J2887_14190) tsaA 3127125..3127961 (+) 837 WP_227171764.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32062.22 Da        Isoelectric Point: 6.6190

>NTDB_id=547760 J2887_RS14215 WP_227171761.1 3124513..3125382(+) (pilD) [Marinobacter sp. CA1]
MPVVDHILSTAWLVYPAIAVLALCIGSFLNVVILRLPKMMEQEWRCQCEEFLELPEKERKDAQRITLASPNSTCPSCGHE
IKPWENIPVVSYLLLRGKCSSCKTPISPRYPIIEALTAILSVATLWLLGPSVATLWTLVLVWTLIALTVIDLDTQLLPDS
MTLPLMWLGLILNHFGLFTDFSSAFWGAIAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQMLPIIILLSSV
VGAVVGIALILFRQHGREVPIPFGPYLAGAGLLALWFGTTLQGWWLGYL

Nucleotide


Download         Length: 870 bp        

>NTDB_id=547760 J2887_RS14215 WP_227171761.1 3124513..3125382(+) (pilD) [Marinobacter sp. CA1]
ATGCCCGTCGTCGACCATATCCTGTCTACCGCATGGCTGGTTTATCCAGCCATTGCTGTTCTTGCCCTTTGCATCGGCAG
CTTCCTGAACGTCGTTATCCTTCGGTTACCGAAGATGATGGAGCAGGAGTGGCGGTGCCAGTGCGAGGAGTTTCTTGAAC
TTCCCGAAAAGGAGCGCAAGGACGCCCAACGCATCACACTGGCCAGCCCCAACAGCACTTGCCCCAGCTGCGGCCATGAG
ATCAAGCCCTGGGAGAATATCCCGGTGGTGAGCTATTTGCTGCTTCGGGGTAAATGTTCGTCCTGCAAAACGCCGATCTC
GCCACGCTACCCCATTATTGAGGCGCTGACGGCGATCCTGTCGGTAGCGACCCTTTGGCTCCTGGGCCCATCCGTGGCCA
CGCTGTGGACGCTGGTGCTGGTGTGGACGCTGATTGCGCTGACCGTGATTGATCTGGATACCCAACTGCTGCCAGACAGC
ATGACGCTGCCACTGATGTGGCTTGGGCTGATCCTCAATCATTTTGGCCTGTTCACCGACTTCAGCAGCGCGTTCTGGGG
GGCAATTGCCGGCTATCTGTCCCTGTGGTCTGTCTACTGGCTGTTCAAGCTGGTCACCGGCAAGGAGGGCATGGGCCATG
GTGATTTCAAGCTTCTGGCGGCACTCGGTGCCTGGCTGGGCTGGCAGATGCTGCCCATCATCATCCTGCTGTCCTCCGTG
GTGGGCGCGGTTGTCGGCATCGCGCTGATCCTGTTCCGCCAGCATGGTCGTGAAGTGCCCATTCCCTTTGGTCCCTACCT
CGCAGGCGCTGGTCTGCTGGCGCTGTGGTTTGGGACCACGCTGCAGGGCTGGTGGCTGGGTTACCTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

52.281

98.616

0.516
  pilD Vibrio cholerae strain A1552

53.237

96.194

0.512
  pilD Vibrio campbellii strain DS40M4

53.069

95.848

0.509
  pilD Acinetobacter nosocomialis M2

51.228

98.616

0.505
  pilD Neisseria gonorrhoeae MS11

53.125

88.581

0.471