Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   J2N86_RS10535 Genome accession   NZ_CP071527
Coordinates   2395694..2396125 (+) Length   143 a.a.
NCBI ID   WP_252579396.1    Uniprot ID   -
Organism   Legionella lytica strain PCM 2298     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2390694..2401125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2N86_RS10525 (J2N86_10535) letS 2391445..2394183 (+) 2739 WP_252579393.1 two-component system sensor histidine kinase LetS Regulator
  J2N86_RS10530 (J2N86_10540) - 2394386..2395621 (+) 1236 WP_252579394.1 6-phosphofructokinase -
  J2N86_RS10535 (J2N86_10545) pilA2 2395694..2396125 (+) 432 WP_252579396.1 pilin Machinery gene
  J2N86_RS10540 (J2N86_10550) - 2396127..2396441 (+) 315 WP_252579397.1 BolA family transcriptional regulator -
  J2N86_RS10545 (J2N86_10555) - 2396539..2397276 (-) 738 WP_252579398.1 outer membrane protein -
  J2N86_RS10550 (J2N86_10560) - 2397389..2399101 (-) 1713 WP_252579399.1 S8 family serine peptidase -
  J2N86_RS10555 (J2N86_10565) - 2399342..2400745 (+) 1404 WP_252579400.1 APC family permease -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15156.57 Da        Isoelectric Point: 9.0588

>NTDB_id=546012 J2N86_RS10535 WP_252579396.1 2395694..2396125(+) (pilA2) [Legionella lytica strain PCM 2298]
MKQKGLTLIELMIVIAIIGILASVAIPSYQDYLIRARVTEGLSLATTAQTTVSENALTGNTDLSMGWSSPTATAIVKNVS
IAKNTGVITINYTPLAQNVAITMSPKSKGELLSAGTPPTTTITWECAVTEKSNNRYVPANCRI

Nucleotide


Download         Length: 432 bp        

>NTDB_id=546012 J2N86_RS10535 WP_252579396.1 2395694..2396125(+) (pilA2) [Legionella lytica strain PCM 2298]
ATGAAACAAAAAGGCTTAACATTAATTGAATTAATGATTGTTATAGCAATTATTGGTATTCTTGCTTCCGTTGCTATCCC
TTCCTATCAAGATTACCTGATTAGAGCCAGAGTAACAGAAGGTTTGAGTTTAGCGACTACAGCCCAAACCACAGTCTCTG
AAAATGCCCTGACCGGAAATACTGATTTAAGTATGGGATGGAGCAGCCCCACGGCTACTGCTATTGTAAAAAATGTCAGT
ATTGCCAAAAACACTGGCGTGATTACTATTAATTACACGCCACTCGCTCAAAATGTTGCAATCACTATGAGCCCCAAATC
CAAAGGGGAACTCCTCAGTGCGGGTACGCCACCTACAACAACGATTACTTGGGAGTGTGCAGTAACTGAAAAAAGTAATA
ACCGATATGTCCCAGCAAATTGTAGGATTTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)

Pilin (bacterial filament) (Pilin)

(35-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

49.324

100

0.51
  pilA2 Legionella pneumophila str. Paris

49.324

100

0.51
  pilA Ralstonia pseudosolanacearum GMI1000

45.223

100

0.497
  comP Acinetobacter baylyi ADP1

41.781

100

0.427
  pilE Neisseria gonorrhoeae MS11

36.81

100

0.42
  pilE Neisseria gonorrhoeae strain FA1090

35.897

100

0.392
  pilA Pseudomonas aeruginosa PAK

35.484

100

0.385