Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I8J32_RS00115 Genome accession   NZ_CP071518
Coordinates   18736..19866 (-) Length   376 a.a.
NCBI ID   WP_200614014.1    Uniprot ID   -
Organism   Agrilutibacter solisilvae strain R19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 13736..24866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8J32_RS00090 (I8J32_000090) - 14126..15043 (-) 918 WP_200614006.1 aspartate carbamoyltransferase catalytic subunit -
  I8J32_RS00095 (I8J32_000095) ruvX 15125..15580 (-) 456 WP_200614007.1 Holliday junction resolvase RuvX -
  I8J32_RS00100 (I8J32_000100) - 15618..16184 (-) 567 WP_200614009.1 YqgE/AlgH family protein -
  I8J32_RS00105 (I8J32_000105) - 16287..18002 (+) 1716 WP_200614011.1 dipeptidyl-peptidase 3 family protein -
  I8J32_RS00110 (I8J32_000110) - 18004..18567 (+) 564 WP_200614012.1 DNA-3-methyladenine glycosylase I -
  I8J32_RS00115 (I8J32_000115) pilU 18736..19866 (-) 1131 WP_200614014.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I8J32_RS00120 (I8J32_000120) - 19974..21012 (-) 1039 Protein_23 type IV pilus twitching motility protein PilT -
  I8J32_RS00125 (I8J32_000125) - 21160..21840 (+) 681 WP_245156372.1 YggS family pyridoxal phosphate-dependent enzyme -
  I8J32_RS00130 (I8J32_000130) proC 21914..22768 (+) 855 WP_200614017.1 pyrroline-5-carboxylate reductase -
  I8J32_RS00135 (I8J32_000135) - 22783..23319 (+) 537 WP_200614019.1 DUF4426 domain-containing protein -
  I8J32_RS00140 (I8J32_000140) - 23360..24163 (-) 804 WP_200614021.1 cobalamin-binding protein -
  I8J32_RS00145 (I8J32_000145) aac(6') 24160..24624 (-) 465 WP_200614022.1 aminoglycoside 6'-N-acetyltransferase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41872.29 Da        Isoelectric Point: 6.8285

>NTDB_id=545903 I8J32_RS00115 WP_200614014.1 18736..19866(-) (pilU) [Agrilutibacter solisilvae strain R19]
MSTIDFTSFLKLMAHQKASDLFITAGMAPSMKVHGKISPITQNPLTPQQSRDLVLNVMTPPQREEFEKTHECNFAIGVTG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELSLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNLNSTGHIIT
IEDPIEFVHKHEGCIVTQREVGIDTDSWDNALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEVHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDAKTLAQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=545903 I8J32_RS00115 WP_200614014.1 18736..19866(-) (pilU) [Agrilutibacter solisilvae strain R19]
ATGAGCACCATCGACTTCACTTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCGGACCTGTTCATCACGGCCGGCAT
GGCGCCCTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACGCAGAACCCGCTGACGCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGCCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCGATTGGCGTCACCGGC
GTCGGCCGCTTCCGCGTCTCGTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CACCGTCGAAGAGTTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTGGGTG
CCACCGGTACCGGCAAGTCGACGTCGCTGGCAGCGATGATCGGCTACCGCAACCTCAATTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCGTGACCCAGCGTGAGGTCGGGATCGACACCGACAG
CTGGGACAACGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCATGCCATCGCTTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGTTGCTGATGGACCTCTCGCTCAACCTGAAGGGCGTGGT
CGCGCAGCAGTTGATCCCGACGCCGGACGGCAAGGCCCGCCGCGTGGCAATGGAAATCCTGCTGGGCACGCCGCTTGTGC
AGGACTACATCCGCGATGGCGAGGTCCACAAGCTGAAGGAAGTGATGAAGGAGTCCGTGCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGTTACGCGGACTCGCAGAACGA
AGTCCGCCTGCGCATCAAGCTGGCCCAGGGCGGAGATGCCAAGACCCTGGCCCAGGGCCTGGATGGTGTGGAAGTGGCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.286

93.085

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364