Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FH974_RS01080 Genome accession   NZ_CP071325
Coordinates   229552..231219 (+) Length   555 a.a.
NCBI ID   WP_047887157.1    Uniprot ID   A0A0J1H1A6
Organism   Photobacterium ganghwense strain C2.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224552..236219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH974_RS01060 (FH974_01060) pilM 226781..227782 (+) 1002 WP_207103441.1 type IV pilus assembly protein PilM -
  FH974_RS01065 (FH974_01065) - 227779..228357 (+) 579 WP_207103442.1 PilN domain-containing protein -
  FH974_RS01070 (FH974_01070) - 228350..228940 (+) 591 WP_207103443.1 type 4a pilus biogenesis protein PilO -
  FH974_RS01075 (FH974_01075) - 228937..229467 (+) 531 WP_047887158.1 pilus assembly protein PilP -
  FH974_RS01080 (FH974_01080) pilQ 229552..231219 (+) 1668 WP_047887157.1 type IV pilus secretin PilQ family protein Machinery gene
  FH974_RS01085 (FH974_01085) aroK 231433..231951 (+) 519 WP_047887156.1 shikimate kinase AroK -
  FH974_RS01090 (FH974_01090) aroB 231972..233054 (+) 1083 WP_047887155.1 3-dehydroquinate synthase -
  FH974_RS01095 (FH974_01095) - 233064..234575 (+) 1512 WP_047887154.1 AAA family ATPase -
  FH974_RS01100 (FH974_01100) - 234683..235510 (+) 828 WP_047887153.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  FH974_RS01105 (FH974_01105) - 235583..235852 (-) 270 WP_047887152.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 60696.47 Da        Isoelectric Point: 4.8279

>NTDB_id=545043 FH974_RS01080 WP_047887157.1 229552..231219(+) (pilQ) [Photobacterium ganghwense strain C2.2]
MLWALMQSSVSFAAAENRIQAIDFTRSTAGHGVLKVDLANARSVVDMRRAGEKLYVDVHDTEIPEDMIYTLDVKEFATRV
QTIETFRTERGVRLVLSMNGTYAYDYTSKDKVLTVTVEKVAKPSSAKAAPSSISYNSKPISINFQDVPVRNVLQLIAEYN
DFNLVVSDSVQGNLTLRLDDVPWKQVLDIILKSKGLDKREQGSVVMIAPKEELAREEQKQLESKRKEEELASLRSEIIQI
NYAKASELGELLQGSGEGVSMLSPRGSLHIDERTNSLIINDIAESLANIRDIVTALDIPVQQVQIEARVVTVNEGDIDEL
GVRWGMLSRNGNNTIGSSIEGNLVSEGLLDLADNRVDQVGDFLNVNLGATKAGASSIAFQVAKIGDVLLDLELSALQAEQ
KAEIISSPRLMTTNKKMAYIEQGTEIPYLEASSSGAVSVGFKKAVLSLMVTPQITPDGKLVLDLVVTQDKKGDVVKTGTG
EAIAIDTQRIGTQVLVENGETVVLGGIYQHSLTNMVRKVPLLGDLPVLGALFRHKLEELGKRELLIFVTPKIINQ

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=545043 FH974_RS01080 WP_047887157.1 229552..231219(+) (pilQ) [Photobacterium ganghwense strain C2.2]
ATGTTATGGGCCCTGATGCAAAGTTCAGTCTCATTTGCTGCCGCAGAAAACCGAATTCAGGCAATCGACTTTACACGCAG
TACGGCCGGGCACGGGGTCCTGAAAGTCGATCTGGCAAACGCCCGTTCTGTGGTCGATATGCGCCGGGCTGGCGAGAAGC
TGTATGTTGATGTGCATGACACGGAGATTCCGGAGGACATGATTTATACCCTCGACGTGAAAGAGTTCGCGACCCGGGTA
CAAACCATCGAAACCTTCCGAACCGAGCGGGGGGTGCGTTTGGTGCTCTCAATGAACGGCACTTATGCCTATGACTACAC
CAGCAAGGATAAAGTGCTGACGGTGACCGTCGAGAAAGTGGCAAAACCAAGCTCAGCCAAGGCCGCGCCGTCATCGATTT
CTTATAACAGCAAGCCCATCTCTATCAACTTCCAGGATGTGCCGGTGCGAAACGTGCTGCAGCTGATCGCTGAGTACAAC
GATTTCAACCTGGTGGTCTCGGATTCGGTGCAGGGCAACCTGACGCTGCGGTTGGACGACGTACCATGGAAGCAGGTGCT
GGATATCATTTTGAAATCCAAAGGGCTGGACAAGCGTGAACAGGGCAGTGTGGTCATGATTGCCCCGAAAGAAGAGCTGG
CGCGAGAAGAGCAGAAACAGCTGGAGTCGAAGCGTAAAGAAGAGGAACTGGCTTCACTGCGCTCGGAGATTATCCAGATT
AACTATGCCAAGGCATCGGAGCTGGGCGAGCTGCTGCAGGGCAGCGGTGAAGGGGTGAGCATGTTGTCACCGCGTGGCTC
GCTGCATATTGATGAACGAACTAACTCGCTGATCATTAATGATATTGCTGAAAGCTTGGCGAATATCCGCGATATCGTGA
CGGCGCTCGACATCCCAGTGCAGCAGGTCCAAATTGAAGCTCGTGTGGTCACGGTGAACGAAGGTGATATTGACGAGCTG
GGGGTTCGCTGGGGGATGCTGAGTCGTAATGGCAACAATACGATCGGTAGCTCCATTGAAGGCAATTTGGTGTCGGAAGG
GCTGCTTGATCTGGCGGATAACCGGGTGGATCAGGTCGGGGATTTCCTCAATGTTAATTTGGGAGCCACCAAAGCGGGGG
CGTCAAGCATCGCTTTCCAGGTCGCCAAAATCGGCGATGTGTTACTGGATCTGGAATTATCGGCCCTGCAGGCGGAGCAG
AAAGCGGAAATCATTTCCAGTCCGCGCCTGATGACCACCAATAAAAAAATGGCCTATATCGAGCAGGGAACCGAAATTCC
GTACTTGGAAGCATCATCCAGCGGCGCGGTATCGGTGGGCTTTAAAAAGGCCGTGCTCAGTCTGATGGTGACCCCGCAGA
TTACTCCGGACGGCAAGCTGGTGCTGGATTTGGTCGTTACCCAGGATAAGAAGGGGGATGTCGTAAAAACCGGGACCGGC
GAAGCGATTGCGATTGATACCCAGCGTATCGGGACCCAGGTTCTGGTTGAAAATGGCGAGACCGTTGTTTTGGGGGGAAT
CTACCAGCATTCGCTGACAAACATGGTGCGTAAAGTGCCATTGCTGGGGGATTTACCGGTATTAGGCGCCTTGTTCCGCC
ATAAGCTGGAAGAGCTGGGCAAGCGTGAATTGCTGATTTTTGTGACGCCAAAGATTATTAATCAATAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(162-210)

Bacterial type II and III secretion system protein (Secretin)

(395-553)

Bacterial type II/III secretion system short domain (Secretin_N)

(236-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1H1A6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.21

100

0.62
  pilQ Vibrio cholerae strain A1552

61.21

100

0.62
  pilQ Vibrio campbellii strain DS40M4

61.151

100

0.613
  comQ Acinetobacter baylyi ADP1

40.696

100

0.422
  pilQ Legionella pneumophila strain Lp02

41.728

98.018

0.409
  pilQ Legionella pneumophila strain ERS1305867

41.728

98.018

0.409
  pilQ Acinetobacter baumannii D1279779

38.605

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.605

100

0.409
  pilQ Pseudomonas aeruginosa PAK

40.072

100

0.404