Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J0W34_RS14525 Genome accession   NZ_CP071321
Coordinates   3111594..3113312 (+) Length   572 a.a.
NCBI ID   WP_230969260.1    Uniprot ID   -
Organism   Nitrogeniibacter aestuarii strain H1-1-2A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3106594..3118312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W34_RS14500 - 3106610..3107065 (+) 456 WP_230969258.1 cytochrome c -
  J0W34_RS14505 - 3107115..3107924 (-) 810 WP_230969259.1 ferritin-like domain-containing protein -
  J0W34_RS14510 ffh 3107938..3109296 (-) 1359 WP_227817188.1 signal recognition particle protein -
  J0W34_RS14515 - 3109335..3110162 (+) 828 WP_227817189.1 inner membrane protein YpjD -
  J0W34_RS14520 - 3110227..3111495 (+) 1269 WP_227817190.1 HlyC/CorC family transporter -
  J0W34_RS14525 pilB 3111594..3113312 (+) 1719 WP_230969260.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J0W34_RS14530 pilC 3113329..3114564 (+) 1236 WP_230969261.1 type II secretion system F family protein Machinery gene
  J0W34_RS14535 pilD 3114581..3115432 (+) 852 WP_230969262.1 A24 family peptidase Machinery gene
  J0W34_RS14540 - 3115534..3115812 (+) 279 WP_227817194.1 FmdB family zinc ribbon protein -
  J0W34_RS14545 - 3115820..3116452 (+) 633 WP_227817195.1 DUF502 domain-containing protein -
  J0W34_RS14550 aspS 3116503..3118299 (+) 1797 WP_230969263.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62068.99 Da        Isoelectric Point: 4.8582

>NTDB_id=545010 J0W34_RS14525 WP_230969260.1 3111594..3113312(+) (pilB) [Nitrogeniibacter aestuarii strain H1-1-2A]
MAANPQVALSGLARALIQQNRISEADAVACTTESKDKVNGFLLEAAKRGIMSEQEVARFAADTFGYPLLDIGALDPQAIL
KGAIDQKLVIKHHVLPVGKRGNRITVALADPTNLRALDEIRFQSGLSVDAVVVEASKLEAAVGKQNESTADTLESLTGDD
FDMDLLDQEGANESESNDDLQGEVDDAPVVRFIQKVLVDAINEGASDIHFEPYEKYYRIRFRTDGILREIAQPPLILREK
IAARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSSLPTLHGEKIVMRILDPSSAMLGIDALGYEPDQKKALLEAI
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKGDSNISTAEDPAEINLPGINQVNVNEKAGLTFSAALKAFLRQDPDIIMVG
EIRDIDTAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCKCKEPMDIPHEALIDA
GFTEDELDGSWKPMGPKGCDACGGSGYKGRVGIYQVMPVSEEMQRIIMSNGNSMDIAAQAELEGVSDLRRSGLRKVKAGM
TSLTEVLATTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=545010 J0W34_RS14525 WP_230969260.1 3111594..3113312(+) (pilB) [Nitrogeniibacter aestuarii strain H1-1-2A]
ATGGCCGCTAATCCTCAAGTCGCACTGTCTGGGCTCGCCCGGGCCCTGATCCAGCAAAACCGCATTTCGGAAGCGGACGC
TGTCGCGTGTACGACCGAGAGCAAGGACAAGGTGAACGGCTTTCTCCTCGAGGCGGCAAAACGCGGGATCATGAGCGAGC
AGGAGGTCGCACGGTTTGCTGCAGACACCTTCGGCTATCCCTTACTCGACATCGGCGCACTGGACCCCCAAGCCATCCTG
AAAGGCGCGATCGATCAGAAGCTCGTCATCAAACATCACGTGCTTCCCGTGGGCAAGCGAGGCAATCGAATCACGGTCGC
GTTGGCCGACCCGACAAACCTGCGGGCGCTGGACGAAATTCGCTTCCAGAGCGGCTTGTCTGTCGATGCAGTGGTGGTTG
AGGCCAGCAAACTCGAGGCTGCGGTTGGCAAACAGAATGAATCGACCGCCGACACACTTGAATCGCTCACCGGCGACGAC
TTCGACATGGACCTCCTCGACCAGGAGGGCGCAAACGAAAGCGAGAGTAATGACGATCTTCAGGGTGAAGTGGATGATGC
ACCGGTCGTGCGCTTCATTCAGAAGGTACTGGTCGATGCCATCAACGAAGGCGCGTCGGACATCCACTTCGAACCCTACG
AAAAGTACTACCGCATCCGGTTCAGAACCGATGGCATTCTGCGTGAGATCGCCCAGCCCCCACTGATTCTCAGAGAAAAG
ATCGCCGCGCGGATCAAGGTCATTTCCCGTCTCGACATCTCGGAGAAACGCGTCCCCCAGGACGGCCGGATGAAACTGGT
TCTGTCGAAGAACAAGGCCATTGATTTCCGGGTCTCCTCCCTGCCGACCCTGCATGGCGAAAAGATCGTCATGCGTATCC
TGGATCCGTCGTCGGCCATGCTGGGTATTGACGCGCTCGGCTACGAGCCGGACCAGAAGAAGGCACTGCTCGAAGCCATC
GAACGCCCCTACGGCATGATTCTGGTCACGGGCCCGACCGGGAGCGGTAAAACAGTCTCGCTCTACACCTGCCTGAATCT
GCTGAACAAGGGTGACTCGAATATCTCGACAGCTGAAGATCCGGCCGAGATCAACTTGCCTGGGATCAATCAGGTCAATG
TCAACGAAAAGGCGGGCCTCACCTTCTCTGCCGCACTCAAGGCGTTTCTGCGGCAGGATCCGGACATCATCATGGTGGGC
GAGATCCGTGACATCGACACTGCAGAAATCTCCATCAAGGCAGCGCAGACCGGCCACCTGGTGTTATCGACCCTCCACAC
CAATGACGCCCCGACCACCCTTGAACGACTGCGCAATATGGGTGTCGCCCCGTTCAACATCGCCTCCTCCGTCATTCTCA
TCACGGCTCAGCGGCTTGCACGCCGGCTGTGCAAATGCAAAGAGCCCATGGACATCCCCCATGAAGCGCTCATTGATGCC
GGCTTCACCGAGGACGAGCTGGACGGCAGCTGGAAACCGATGGGCCCCAAGGGCTGTGACGCATGCGGGGGCAGCGGATA
CAAGGGGCGCGTCGGCATCTACCAGGTCATGCCGGTTTCCGAAGAAATGCAGCGCATCATCATGAGTAACGGCAATTCAA
TGGATATCGCTGCACAGGCTGAACTCGAAGGGGTCTCCGATCTCCGACGCTCGGGACTGAGAAAGGTCAAAGCCGGCATG
ACGTCCTTGACCGAAGTCCTCGCAACCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.643

100

0.566
  pilB Acinetobacter baylyi ADP1

53.954

99.476

0.537
  pilF Neisseria gonorrhoeae MS11

53.59

99.825

0.535
  pilB Legionella pneumophila strain ERS1305867

50.788

99.825

0.507
  pilB Vibrio cholerae strain A1552

48.763

98.951

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

48.375

91.434

0.442
  pilB Vibrio campbellii strain DS40M4

48.375

91.434

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.011

95.455

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

41.717

87.587

0.365
  pilF Thermus thermophilus HB27

39.286

93.007

0.365