Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J0W34_RS14535 Genome accession   NZ_CP071321
Coordinates   3114581..3115432 (+) Length   283 a.a.
NCBI ID   WP_230969262.1    Uniprot ID   -
Organism   Nitrogeniibacter aestuarii strain H1-1-2A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3109581..3120432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W34_RS14520 - 3110227..3111495 (+) 1269 WP_227817190.1 HlyC/CorC family transporter -
  J0W34_RS14525 pilB 3111594..3113312 (+) 1719 WP_230969260.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J0W34_RS14530 pilC 3113329..3114564 (+) 1236 WP_230969261.1 type II secretion system F family protein Machinery gene
  J0W34_RS14535 pilD 3114581..3115432 (+) 852 WP_230969262.1 A24 family peptidase Machinery gene
  J0W34_RS14540 - 3115534..3115812 (+) 279 WP_227817194.1 FmdB family zinc ribbon protein -
  J0W34_RS14545 - 3115820..3116452 (+) 633 WP_227817195.1 DUF502 domain-containing protein -
  J0W34_RS14550 aspS 3116503..3118299 (+) 1797 WP_230969263.1 aspartate--tRNA ligase -
  J0W34_RS14555 - 3118421..3120046 (+) 1626 WP_227817197.1 response regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30612.06 Da        Isoelectric Point: 6.3720

>NTDB_id=545012 J0W34_RS14535 WP_230969262.1 3114581..3115432(+) (pilD) [Nitrogeniibacter aestuarii strain H1-1-2A]
MSFLDIEPVVLAAIAGVLGLFVGSFLNVVIHRLPIMLEHEWEDEAADLKGEEPQTRPRFNLATPRSRCPHCGTQVSAIDN
VPVLSFLILRGKCRHCSATISHRYPAVEVLTAALSAAAAWHFGPTLATAGALVFIWALIALTFIDLDTQLLPDNITLPLV
WLGLLFNLVGTYATLNDAVVGAVAGYMSLWLVFQLFRLVTGKEGMGYGDFKLLAAIGAWLGWQMLPVTILLSSVVGAVVG
ISLMVFTKHGRDNPIPFGPYLAAAGLLALFFGREINTQYLGLL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=545012 J0W34_RS14535 WP_230969262.1 3114581..3115432(+) (pilD) [Nitrogeniibacter aestuarii strain H1-1-2A]
GTGTCTTTTCTCGATATCGAACCGGTCGTACTGGCAGCGATCGCAGGCGTCCTCGGTCTGTTCGTCGGCAGTTTCCTCAA
TGTCGTTATCCACCGTCTTCCCATCATGCTCGAACATGAATGGGAGGACGAGGCGGCAGATCTCAAGGGCGAAGAACCTC
AGACGAGACCTCGTTTCAATCTCGCGACACCACGCTCACGCTGTCCGCACTGCGGTACGCAGGTATCCGCGATCGACAAC
GTTCCGGTACTGAGCTTTCTGATTCTCAGAGGGAAGTGTCGCCACTGCTCGGCGACCATCAGTCACCGCTACCCCGCAGT
GGAAGTGCTGACGGCCGCCCTGTCTGCCGCAGCCGCCTGGCACTTCGGTCCTACACTGGCGACAGCAGGGGCATTGGTCT
TCATCTGGGCCCTGATTGCTTTGACCTTCATCGACCTGGACACCCAATTGCTGCCCGACAACATCACACTGCCTTTAGTG
TGGCTCGGCCTGCTCTTCAACCTCGTCGGAACCTACGCCACGCTCAACGATGCAGTGGTCGGTGCGGTTGCCGGATACAT
GAGCCTCTGGCTCGTGTTCCAGCTTTTCCGTCTGGTCACGGGCAAGGAAGGGATGGGTTATGGCGACTTCAAACTACTGG
CGGCCATCGGTGCCTGGCTGGGCTGGCAAATGTTACCGGTGACCATCTTGCTCTCATCGGTCGTTGGTGCCGTCGTCGGC
ATCAGTCTGATGGTGTTCACCAAGCACGGGCGAGACAACCCGATTCCTTTTGGTCCTTATCTGGCGGCCGCCGGTCTGCT
CGCCCTGTTCTTCGGGCGTGAAATCAACACTCAATACCTCGGCCTCCTTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(132-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.65

100

0.527
  pilD Vibrio campbellii strain DS40M4

51.237

100

0.512
  pilD Acinetobacter baumannii D1279779

50.558

95.053

0.481
  pilD Acinetobacter nosocomialis M2

49.814

95.053

0.473
  pilD Neisseria gonorrhoeae MS11

49.627

94.7

0.47