Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   J0J35_RS00160 Genome accession   NZ_CP071301
Coordinates   25924..26562 (+) Length   212 a.a.
NCBI ID   WP_206917000.1    Uniprot ID   -
Organism   Lactococcus sp. LG606     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1..28792 25924..26562 within 0


Gene organization within MGE regions


Location: 1..28792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J35_RS00010 (J0J35_00010) terL 1..1392 (+) 1392 WP_206916950.1 phage terminase large subunit -
  J0J35_RS00015 (J0J35_00015) - 1393..2739 (+) 1347 WP_206916953.1 phage portal protein -
  J0J35_RS00020 (J0J35_00020) - 2743..3783 (+) 1041 WP_206916955.1 minor capsid protein -
  J0J35_RS00025 (J0J35_00025) - 3790..4044 (+) 255 WP_206916957.1 hypothetical protein -
  J0J35_RS00030 (J0J35_00030) - 4161..4820 (+) 660 WP_206916958.1 DUF4355 domain-containing protein -
  J0J35_RS00035 (J0J35_00035) - 4822..5643 (+) 822 WP_206916959.1 N4-gp56 family major capsid protein -
  J0J35_RS00040 (J0J35_00040) - 5643..5840 (+) 198 WP_206916961.1 HeH/LEM domain-containing protein -
  J0J35_RS00045 (J0J35_00045) - 5827..6159 (+) 333 WP_206916963.1 phage head-tail connector protein -
  J0J35_RS00050 (J0J35_00050) - 6156..6467 (+) 312 WP_206916965.1 hypothetical protein -
  J0J35_RS00055 (J0J35_00055) - 6464..6802 (+) 339 WP_206916968.1 HK97-gp10 family putative phage morphogenesis protein -
  J0J35_RS00060 (J0J35_00060) - 6799..7188 (+) 390 WP_206916970.1 phage capsid protein -
  J0J35_RS00065 (J0J35_00065) - 7199..7714 (+) 516 WP_206916971.1 phage major tail protein, TP901-1 family -
  J0J35_RS00070 (J0J35_00070) - 7817..8167 (+) 351 WP_206916972.1 tail assembly chaperone -
  J0J35_RS00075 (J0J35_00075) - 8251..8520 (+) 270 WP_019292468.1 hypothetical protein -
  J0J35_RS00080 (J0J35_00080) - 8536..11148 (+) 2613 WP_206916973.1 tape measure protein -
  J0J35_RS00085 (J0J35_00085) - 11157..11918 (+) 762 WP_206916976.1 distal tail protein Dit -
  J0J35_RS00090 (J0J35_00090) - 11936..14788 (+) 2853 WP_370569311.1 phage tail spike protein -
  J0J35_RS00095 (J0J35_00095) - 14802..16766 (+) 1965 WP_206916982.1 BppU family phage baseplate upper protein -
  J0J35_RS00100 (J0J35_00100) - 16970..17122 (+) 153 WP_206916985.1 hypothetical protein -
  J0J35_RS00105 (J0J35_00105) - 17204..17431 (+) 228 WP_206916988.1 hemolysin XhlA family protein -
  J0J35_RS00110 (J0J35_00110) - 17435..17695 (+) 261 WP_206916990.1 phage holin -
  J0J35_RS00115 (J0J35_00115) - 17692..18759 (+) 1068 WP_206916992.1 LysM peptidoglycan-binding domain-containing protein -
  J0J35_RS00120 (J0J35_00120) - 18858..19820 (+) 963 WP_206916994.1 DUF3644 domain-containing protein -
  J0J35_RS00125 (J0J35_00125) - 19995..20177 (-) 183 WP_206916996.1 hypothetical protein -
  J0J35_RS00130 (J0J35_00130) - 20428..20619 (+) 192 WP_014024875.1 hypothetical protein -
  J0J35_RS00135 (J0J35_00135) - 20954..21064 (+) 111 WP_207781255.1 hypothetical protein -
  J0J35_RS00145 (J0J35_00145) - 22178..22822 (+) 645 WP_206916998.1 CPBP family intramembrane glutamic endopeptidase -
  J0J35_RS00150 (J0J35_00150) - 22969..24525 (+) 1557 WP_019292098.1 ABC-F family ATP-binding cassette domain-containing protein -
  J0J35_RS00155 (J0J35_00155) - 24691..25866 (+) 1176 WP_019292097.1 SLC13 family permease -
  J0J35_RS00160 (J0J35_00160) comEA 25924..26562 (+) 639 WP_206917000.1 helix-hairpin-helix domain-containing protein Machinery gene
  J0J35_RS00165 (J0J35_00165) comEC 26582..28792 (+) 2211 WP_206917001.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene

Sequence

Protein


Download         Length: 212 a.a.        Molecular weight: 23541.71 Da        Isoelectric Point: 5.5494

>NTDB_id=544784 J0J35_RS00160 WP_206917000.1 25924..26562(+) (comEA) [Lactococcus sp. LG606]
MKKISEYVKENWKIAVGIVLALTILGAFYWNKKVLDIPKQEEKHSEWAELSKEKEATGKKKEEKEADENEIVVDVKGAVK
KPGIYKLSSKKRVSDAVASAGGFTEEAERKGINLAKKLQDEAEVYVPVRGETDTNHLLETKDNTSKTAEKININTADLTE
LQQLSGVGAKKAQDILDYRSENGSFESVEELTKVSGFGPKTLEKLKEAITVD

Nucleotide


Download         Length: 639 bp        

>NTDB_id=544784 J0J35_RS00160 WP_206917000.1 25924..26562(+) (comEA) [Lactococcus sp. LG606]
ATGAAAAAAATATCAGAATATGTAAAAGAAAATTGGAAAATCGCTGTTGGAATTGTTTTGGCATTGACTATTTTAGGGGC
TTTTTATTGGAATAAAAAAGTTTTAGATATCCCAAAGCAAGAAGAAAAGCATTCCGAGTGGGCGGAGCTGAGCAAGGAAA
AAGAAGCTACAGGAAAGAAAAAAGAAGAAAAAGAAGCAGATGAGAATGAAATCGTCGTTGATGTCAAAGGCGCTGTCAAA
AAGCCAGGCATCTACAAGTTATCCTCCAAAAAGCGTGTATCTGATGCAGTAGCAAGTGCTGGGGGCTTTACAGAAGAAGC
AGAAAGGAAGGGAATAAATCTGGCAAAAAAATTACAAGATGAAGCAGAGGTCTATGTTCCAGTGAGAGGAGAAACAGATA
CAAATCACTTGTTAGAAACCAAAGATAATACCAGTAAAACAGCTGAAAAAATTAATATTAATACAGCTGATTTAACTGAA
TTGCAGCAGCTTAGTGGTGTAGGAGCAAAAAAAGCGCAAGATATTCTGGATTACCGTTCTGAAAATGGTTCTTTTGAGTC
AGTGGAAGAATTGACCAAGGTAAGTGGCTTTGGTCCTAAAACATTAGAAAAGTTAAAAGAAGCAATCACTGTAGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(72-126)

Helix-hairpin-helix motif (HHH_3)

(148-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

46.154

100

0.481
  comEA/celA/cilE Streptococcus pneumoniae D39

45.872

100

0.472
  comEA/celA/cilE Streptococcus pneumoniae Rx1

45.872

100

0.472
  comEA/celA/cilE Streptococcus pneumoniae R6

45.872

100

0.472
  comEA/celA/cilE Streptococcus mitis SK321

45.161

100

0.462
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

45.161

100

0.462
  comEA/celA/cilE Streptococcus mitis NCTC 12261

44.7

100

0.458
  comEA Latilactobacillus sakei subsp. sakei 23K

36.681

100

0.396
  comEA Streptococcus thermophilus LMD-9

35.242

100

0.377