Detailed information NTG0544785

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: J0J35_RS00165
• Genome accession: NZ_CP071301
• Coordinates: 26582..28792 (+)
• Length: 736 a.a.
• NCBI ID: WP_206917001.1
• Uniprot ID: -
• Organism: Lactococcus sp. LG606
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	1..28792	26582..28792	within	0

Gene organization within MGE regions

Location: 1..28792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J0J35_RS00010 (J0J35_00010)	terL	1..1392 (+)	1392	WP_206916950.1	phage terminase large subunit	-
J0J35_RS00015 (J0J35_00015)	-	1393..2739 (+)	1347	WP_206916953.1	phage portal protein	-
J0J35_RS00020 (J0J35_00020)	-	2743..3783 (+)	1041	WP_206916955.1	minor capsid protein	-
J0J35_RS00025 (J0J35_00025)	-	3790..4044 (+)	255	WP_206916957.1	hypothetical protein	-
J0J35_RS00030 (J0J35_00030)	-	4161..4820 (+)	660	WP_206916958.1	DUF4355 domain-containing protein	-
J0J35_RS00035 (J0J35_00035)	-	4822..5643 (+)	822	WP_206916959.1	N4-gp56 family major capsid protein	-
J0J35_RS00040 (J0J35_00040)	-	5643..5840 (+)	198	WP_206916961.1	HeH/LEM domain-containing protein	-
J0J35_RS00045 (J0J35_00045)	-	5827..6159 (+)	333	WP_206916963.1	phage head-tail connector protein	-
J0J35_RS00050 (J0J35_00050)	-	6156..6467 (+)	312	WP_206916965.1	hypothetical protein	-
J0J35_RS00055 (J0J35_00055)	-	6464..6802 (+)	339	WP_206916968.1	HK97-gp10 family putative phage morphogenesis protein	-
J0J35_RS00060 (J0J35_00060)	-	6799..7188 (+)	390	WP_206916970.1	phage capsid protein	-
J0J35_RS00065 (J0J35_00065)	-	7199..7714 (+)	516	WP_206916971.1	phage major tail protein, TP901-1 family	-
J0J35_RS00070 (J0J35_00070)	-	7817..8167 (+)	351	WP_206916972.1	tail assembly chaperone	-
J0J35_RS00075 (J0J35_00075)	-	8251..8520 (+)	270	WP_019292468.1	hypothetical protein	-
J0J35_RS00080 (J0J35_00080)	-	8536..11148 (+)	2613	WP_206916973.1	tape measure protein	-
J0J35_RS00085 (J0J35_00085)	-	11157..11918 (+)	762	WP_206916976.1	distal tail protein Dit	-
J0J35_RS00090 (J0J35_00090)	-	11936..14788 (+)	2853	WP_370569311.1	phage tail spike protein	-
J0J35_RS00095 (J0J35_00095)	-	14802..16766 (+)	1965	WP_206916982.1	BppU family phage baseplate upper protein	-
J0J35_RS00100 (J0J35_00100)	-	16970..17122 (+)	153	WP_206916985.1	hypothetical protein	-
J0J35_RS00105 (J0J35_00105)	-	17204..17431 (+)	228	WP_206916988.1	hemolysin XhlA family protein	-
J0J35_RS00110 (J0J35_00110)	-	17435..17695 (+)	261	WP_206916990.1	phage holin	-
J0J35_RS00115 (J0J35_00115)	-	17692..18759 (+)	1068	WP_206916992.1	LysM peptidoglycan-binding domain-containing protein	-
J0J35_RS00120 (J0J35_00120)	-	18858..19820 (+)	963	WP_206916994.1	DUF3644 domain-containing protein	-
J0J35_RS00125 (J0J35_00125)	-	19995..20177 (-)	183	WP_206916996.1	hypothetical protein	-
J0J35_RS00130 (J0J35_00130)	-	20428..20619 (+)	192	WP_014024875.1	hypothetical protein	-
J0J35_RS00135 (J0J35_00135)	-	20954..21064 (+)	111	WP_207781255.1	hypothetical protein	-
J0J35_RS00145 (J0J35_00145)	-	22178..22822 (+)	645	WP_206916998.1	CPBP family intramembrane glutamic endopeptidase	-
J0J35_RS00150 (J0J35_00150)	-	22969..24525 (+)	1557	WP_019292098.1	ABC-F family ATP-binding cassette domain-containing protein	-
J0J35_RS00155 (J0J35_00155)	-	24691..25866 (+)	1176	WP_019292097.1	SLC13 family permease	-
J0J35_RS00160 (J0J35_00160)	comEA	25924..26562 (+)	639	WP_206917000.1	helix-hairpin-helix domain-containing protein	Machinery gene
J0J35_RS00165 (J0J35_00165)	comEC	26582..28792 (+)	2211	WP_206917001.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene

Sequence

Protein

• Length: 736 a.a.
• Molecular weight: 85099.03 Da
• Isoelectric Point: 9.7443

>NTDB_id=544785 J0J35_RS00165 WP_206917001.1 26582..28792(+) (comEC) [Lactococcus sp. LG606]
MKQKFTLIYLVYLLVFVYFLIFSFSWPLLILSLFSLFIAFYRQYYSLLALLLCFSFFLLMVKKETEVQEIQQPEKISKIT
PYMDTLQVNGNRLSFRGEVNGRDFQAFYTLTSEKEKEYFKNLRIKGIFYIEGTLEIPEEQRNFSGFDYRKYLRTQGISRL
LKIEKIEGFEEKHDFDLRLERRKAILWTQEHFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSRKLVYFIQIPFSIFYAFLTGLSLSILRALLQKNIPIKNSSDRFSITFLLLMIFSPYFLLTTGGQLTML
YSFLITFFSQKFNELKGLKRALLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFDFLLLPGLLFVFLLSLFGIDLT
LLNPFFIILESIVRLVGSLFDKPIVFGKPNLIFLLLLFMLSGLALDFYRKRNWNLVVIIVLFFLFFLVKNPKHPSITMVD
VGQGDSILLQDRWNSQNILIDTGGRLKIRADEQWKEGIYKSNAESTLLPYLRASGVSKVDVLIVTHPDEDHIGDLESVAQ
KVPIKNIYVSASALEKKNFVKKLSSLKSQIHIVQEGDKLPIFNSYLRFLTTGGEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVKLQKEYPHLEVDILKVGHHGSKTSTKEGFLEAIKPKVALISAGKNNRYKHPSQETLHQLTARGIKIYRTDQQGA
IRLLYKKGTWQIQTVK

Nucleotide

• Length: 2211 bp

>NTDB_id=544785 J0J35_RS00165 WP_206917001.1 26582..28792(+) (comEC) [Lactococcus sp. LG606]
ATGAAACAAAAATTTACGCTTATCTATTTAGTATATCTCTTAGTTTTTGTTTATTTTCTTATTTTCAGTTTTTCATGGCC
TTTACTTATTCTTAGTTTGTTTAGTCTCTTCATCGCTTTTTATCGTCAATACTATAGTTTGTTAGCCCTTTTACTGTGCT
TTAGTTTCTTCTTACTCATGGTAAAAAAAGAGACAGAAGTACAGGAAATACAACAACCAGAAAAAATTTCGAAGATTACC
CCTTATATGGATACTCTTCAAGTGAATGGAAACAGACTCAGTTTCAGGGGAGAAGTGAACGGAAGAGATTTCCAAGCTTT
TTACACATTGACTTCGGAAAAAGAAAAAGAATACTTTAAAAATCTTAGGATAAAGGGGATTTTTTACATTGAAGGCACAT
TAGAGATACCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACGCAAGGTATTTCCCGACTT
CTTAAAATAGAAAAAATTGAAGGATTTGAAGAAAAACATGACTTTGATTTACGGCTGGAACGCCGAAAAGCCATTCTTTG
GACACAAGAACATTTTCCTTCTCCCATGTCGTCCTATATGACAGGGCTTCTTTTTGGTTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTATACTAGCCTCGGTATTATCCATTTATTTGCCCTGTCAGGGATGCAGGTGAATTTTTTTATTGAA
CTTCTTCGAAATATTTTATTAAGGCTAGGTTTATCAAGAAAGTTGGTCTATTTTATCCAAATTCCCTTTTCGATATTTTA
TGCTTTTCTTACTGGCTTATCTCTCTCAATCTTACGTGCGCTTCTTCAAAAAAATATACCTATTAAAAATTCAAGCGATA
GATTTTCTATCACTTTTCTTCTTTTAATGATATTCAGTCCATATTTCCTACTGACAACAGGAGGACAACTGACGATGCTC
TATTCTTTCTTAATCACATTTTTTTCACAAAAATTCAATGAACTAAAAGGATTAAAAAGAGCATTACTTGATTCTACTGT
TCTAGCTGTTGGGGTACTTCCGCTTTTGATTTTTCACTTCCATTCTTTCCAACCATTATCGATTATATTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTTTGCCAGGTTTACTTTTTGTCTTTCTCCTTTCTTTATTCGGCATTGATCTTACA
TTACTTAATCCTTTTTTTATTATTTTAGAATCAATAGTAAGACTGGTTGGCAGTTTATTTGATAAACCGATTGTATTTGG
CAAACCAAATCTTATTTTTTTACTTTTGCTTTTTATGCTAAGTGGACTTGCACTCGATTTTTACAGAAAAAGAAACTGGA
ACTTAGTTGTAATAATTGTTCTTTTCTTTCTTTTTTTCCTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGGGCCAAGGAGACAGTATTCTCTTGCAAGACAGGTGGAACAGTCAAAATATCTTAATTGATACTGGCGGTAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATTTATAAGAGCAATGCTGAAAGTACACTTCTACCTTATCTGAGAGCGA
GTGGAGTGAGCAAAGTAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACTTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATATTTATGTCTCAGCTAGTGCATTAGAGAAGAAAAATTTTGTTAAAAAACTAAGCAGCTTAAA
AAGTCAGATTCACATTGTGCAAGAGGGAGACAAGCTCCCCATATTTAACAGTTATCTCCGGTTTCTAACGACAGGAGGTG
AAGGAAAAAGCGATAATGATAATTCTTTAGTGACGTTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGTGACTTA
GAAGAAGAGGGAGAGGTTAAACTCCAGAAAGAATATCCACATTTAGAGGTTGATATCCTAAAAGTTGGACATCATGGCAG
TAAGACTTCTACAAAGGAAGGTTTTTTAGAAGCGATTAAGCCTAAGGTTGCTCTTATTTCTGCTGGAAAGAATAATCGAT
ATAAACATCCGAGTCAAGAAACGCTTCACCAATTAACTGCACGAGGAATAAAAATTTATCGGACAGACCAGCAAGGAGCA
ATCCGTCTCCTTTATAAAAAAGGCACTTGGCAAATTCAAACAGTAAAGTAA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (481-689)

• Domain of unknown function (DUF4131) (DUF4131) (23-167)

• Competence protein (Competence) (203-445)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Lactococcus lactis subsp. cremoris KW2	52.797	99.592	0.526
comEC/celB	Streptococcus mitis SK321	45.101	100	0.457
comEC/celB	Streptococcus pneumoniae TIGR4	43.876	100	0.443
comEC/celB	Streptococcus mitis NCTC 12261	43.801	100	0.442
comEC/celB	Streptococcus pneumoniae Rx1	43.472	100	0.439
comEC/celB	Streptococcus pneumoniae D39	43.472	100	0.439
comEC/celB	Streptococcus pneumoniae R6	43.472	100	0.439