Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOE88_RS18780 Genome accession   NZ_CP086662
Coordinates   3919390..3920775 (-) Length   461 a.a.
NCBI ID   WP_001025154.1    Uniprot ID   -
Organism   Escherichia coli strain E2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914390..3925775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOE88_RS18745 (LOE88_18745) mutT 3914836..3915225 (+) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -
  LOE88_RS18750 (LOE88_18750) yacG 3915325..3915522 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  LOE88_RS18755 (LOE88_18755) zapD 3915532..3916275 (-) 744 WP_001194734.1 cell division protein ZapD -
  LOE88_RS18760 (LOE88_18760) coaE 3916275..3916895 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  LOE88_RS18765 (LOE88_18765) - 3916920..3916964 (+) 45 WP_120795372.1 protein YacM -
  LOE88_RS18770 (LOE88_18770) guaC 3917120..3918163 (+) 1044 WP_001217338.1 GMP reductase -
  LOE88_RS18775 (LOE88_18775) hofC 3918198..3919400 (-) 1203 WP_000157234.1 protein transport protein HofC -
  LOE88_RS18780 (LOE88_18780) pilB 3919390..3920775 (-) 1386 WP_001025154.1 type II secretion system protein GspE Machinery gene
  LOE88_RS18785 (LOE88_18785) pilA 3920785..3921225 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LOE88_RS18790 (LOE88_18790) nadC 3921428..3922321 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LOE88_RS18795 (LOE88_18795) ampD 3922409..3922960 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LOE88_RS18800 (LOE88_18800) ampE 3922957..3923811 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LOE88_RS18805 (LOE88_18805) aroP 3923854..3925227 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50521.23 Da        Isoelectric Point: 6.4961

>NTDB_id=543979 LOE88_RS18780 WP_001025154.1 3919390..3920775(-) (pilB) [Escherichia coli strain E2]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=543979 LOE88_RS18780 WP_001025154.1 3919390..3920775(-) (pilB) [Escherichia coli strain E2]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCATTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAATCAGACGCAAATCCATCC
ACGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.769

100

0.449
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.831

88.72

0.362