Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LOE88_RS18785 Genome accession   NZ_CP086662
Coordinates   3920785..3921225 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain E2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3915785..3926225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOE88_RS18760 (LOE88_18760) coaE 3916275..3916895 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  LOE88_RS18765 (LOE88_18765) - 3916920..3916964 (+) 45 WP_120795372.1 protein YacM -
  LOE88_RS18770 (LOE88_18770) guaC 3917120..3918163 (+) 1044 WP_001217338.1 GMP reductase -
  LOE88_RS18775 (LOE88_18775) hofC 3918198..3919400 (-) 1203 WP_000157234.1 protein transport protein HofC -
  LOE88_RS18780 (LOE88_18780) pilB 3919390..3920775 (-) 1386 WP_001025154.1 type II secretion system protein GspE Machinery gene
  LOE88_RS18785 (LOE88_18785) pilA 3920785..3921225 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LOE88_RS18790 (LOE88_18790) nadC 3921428..3922321 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LOE88_RS18795 (LOE88_18795) ampD 3922409..3922960 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LOE88_RS18800 (LOE88_18800) ampE 3922957..3923811 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  LOE88_RS18805 (LOE88_18805) aroP 3923854..3925227 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=543980 LOE88_RS18785 WP_000360904.1 3920785..3921225(-) (pilA) [Escherichia coli strain E2]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=543980 LOE88_RS18785 WP_000360904.1 3920785..3921225(-) (pilA) [Escherichia coli strain E2]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363